Abstract
The effect of methanol on the kinetically controlled synthesis of cephalexin by free and immobilized penicillin G acylase (PGA) was investigated. Catalytic and hydrophobic membranes were obtained by chemical grafting, activation, and PGA immobilization on hydrophobic nylon supports. Butyl methacrylate (BMA) was used as graft monomer. Increasing concentrations of methanol were found to cause a greater deleterious effect on the activity of free than on that of the immobilized enzyme. Methanol, however, improved the kinetic stability of cephalexin synthesized by free PGA, resulting in higher maximum yields. By contrast, immobilized PGA reached 100% yields even in the absence of the cosolvent. Cephalexin synthesis by the catalytic membrane was also performed in a non-isothermal bioreactor. Under these conditions, a 94% increase of the synthetic activity and complete conversion of the limiting substrate to cephalexin were obtained. The addition of methanol reduced the non-isothermal activity increase. The physical cause responsible for the non-isothermal behavior of the hydrophobic catalytic membrane was identified in the process of thermodialysis.
| Original language | English |
|---|---|
| Pages (from-to) | 975-985 |
| Number of pages | 11 |
| Journal | Biotechnology Progress |
| Volume | 18 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Sep 2002 |
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ASJC Scopus subject areas
- Food Science
- Biotechnology
- Microbiology
Cite this
Enzyme reaction engineering : Effect of methanol on the synthesis of antibiotics catalyzed by immobilized penicillin G acylase under isothermal and non-isothermal conditions. / Travascio, P.; Zito, E.; Portaccio, M.; Diano, N.; Grano, V.; Di Martino, S.; Bertolini, T.; Rossi, S.; Mita, D. G.
In: Biotechnology Progress, Vol. 18, No. 5, 09.2002, p. 975-985.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enzyme reaction engineering
T2 - Effect of methanol on the synthesis of antibiotics catalyzed by immobilized penicillin G acylase under isothermal and non-isothermal conditions
AU - Travascio, P.
AU - Zito, E.
AU - Portaccio, M.
AU - Diano, N.
AU - Grano, V.
AU - Di Martino, S.
AU - Bertolini, T.
AU - Rossi, S.
AU - Mita, D. G.
PY - 2002/9
Y1 - 2002/9
N2 - The effect of methanol on the kinetically controlled synthesis of cephalexin by free and immobilized penicillin G acylase (PGA) was investigated. Catalytic and hydrophobic membranes were obtained by chemical grafting, activation, and PGA immobilization on hydrophobic nylon supports. Butyl methacrylate (BMA) was used as graft monomer. Increasing concentrations of methanol were found to cause a greater deleterious effect on the activity of free than on that of the immobilized enzyme. Methanol, however, improved the kinetic stability of cephalexin synthesized by free PGA, resulting in higher maximum yields. By contrast, immobilized PGA reached 100% yields even in the absence of the cosolvent. Cephalexin synthesis by the catalytic membrane was also performed in a non-isothermal bioreactor. Under these conditions, a 94% increase of the synthetic activity and complete conversion of the limiting substrate to cephalexin were obtained. The addition of methanol reduced the non-isothermal activity increase. The physical cause responsible for the non-isothermal behavior of the hydrophobic catalytic membrane was identified in the process of thermodialysis.
AB - The effect of methanol on the kinetically controlled synthesis of cephalexin by free and immobilized penicillin G acylase (PGA) was investigated. Catalytic and hydrophobic membranes were obtained by chemical grafting, activation, and PGA immobilization on hydrophobic nylon supports. Butyl methacrylate (BMA) was used as graft monomer. Increasing concentrations of methanol were found to cause a greater deleterious effect on the activity of free than on that of the immobilized enzyme. Methanol, however, improved the kinetic stability of cephalexin synthesized by free PGA, resulting in higher maximum yields. By contrast, immobilized PGA reached 100% yields even in the absence of the cosolvent. Cephalexin synthesis by the catalytic membrane was also performed in a non-isothermal bioreactor. Under these conditions, a 94% increase of the synthetic activity and complete conversion of the limiting substrate to cephalexin were obtained. The addition of methanol reduced the non-isothermal activity increase. The physical cause responsible for the non-isothermal behavior of the hydrophobic catalytic membrane was identified in the process of thermodialysis.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036750876&partnerID=8YFLogxK
U2 - 10.1021/bp010170v
DO - 10.1021/bp010170v
M3 - Article
C2 - 12363348
AN - SCOPUS:0036750876
VL - 18
SP - 975
EP - 985
JO - Biotechnology Progress
JF - Biotechnology Progress
SN - 8756-7938
IS - 5
ER -