Enzyme reaction engineering: Effect of methanol on the synthesis of antibiotics catalyzed by immobilized penicillin G acylase under isothermal and non-isothermal conditions

P. Travascio, E. Zito, M. Portaccio, N. Diano, V. Grano, S. Di Martino, T. Bertolini, S. Rossi, D. G. Mita

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The effect of methanol on the kinetically controlled synthesis of cephalexin by free and immobilized penicillin G acylase (PGA) was investigated. Catalytic and hydrophobic membranes were obtained by chemical grafting, activation, and PGA immobilization on hydrophobic nylon supports. Butyl methacrylate (BMA) was used as graft monomer. Increasing concentrations of methanol were found to cause a greater deleterious effect on the activity of free than on that of the immobilized enzyme. Methanol, however, improved the kinetic stability of cephalexin synthesized by free PGA, resulting in higher maximum yields. By contrast, immobilized PGA reached 100% yields even in the absence of the cosolvent. Cephalexin synthesis by the catalytic membrane was also performed in a non-isothermal bioreactor. Under these conditions, a 94% increase of the synthetic activity and complete conversion of the limiting substrate to cephalexin were obtained. The addition of methanol reduced the non-isothermal activity increase. The physical cause responsible for the non-isothermal behavior of the hydrophobic catalytic membrane was identified in the process of thermodialysis.

Original languageEnglish
Pages (from-to)975-985
Number of pages11
JournalBiotechnology Progress
Volume18
Issue number5
DOIs
Publication statusPublished - Sep 2002

Fingerprint

Penicillin Amidase
cephalexin
Cephalexin
benzylpenicillin
enzymatic reactions
Methanol
engineering
methanol
antibiotics
Anti-Bacterial Agents
synthesis
Enzymes
Membranes
Immobilized Enzymes
immobilized enzymes
Nylons
Bioreactors
nylon
bioreactors
Immobilization

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Enzyme reaction engineering : Effect of methanol on the synthesis of antibiotics catalyzed by immobilized penicillin G acylase under isothermal and non-isothermal conditions. / Travascio, P.; Zito, E.; Portaccio, M.; Diano, N.; Grano, V.; Di Martino, S.; Bertolini, T.; Rossi, S.; Mita, D. G.

In: Biotechnology Progress, Vol. 18, No. 5, 09.2002, p. 975-985.

Research output: Contribution to journalArticle

Travascio, P. ; Zito, E. ; Portaccio, M. ; Diano, N. ; Grano, V. ; Di Martino, S. ; Bertolini, T. ; Rossi, S. ; Mita, D. G. / Enzyme reaction engineering : Effect of methanol on the synthesis of antibiotics catalyzed by immobilized penicillin G acylase under isothermal and non-isothermal conditions. In: Biotechnology Progress. 2002 ; Vol. 18, No. 5. pp. 975-985.
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AU - Zito, E.

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AU - Diano, N.

AU - Grano, V.

AU - Di Martino, S.

AU - Bertolini, T.

AU - Rossi, S.

AU - Mita, D. G.

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