Abstract
Whether human IL-10-producing regulatory T cells ("Tr1") represent a distinct differentiation lineage or an unstable activation stage remains a key unsolved issue. Here, we report that Eomesodermin (Eomes) acted as a lineage-defining transcription factor in human IFN-γ/IL-10 coproducing Tr1-like cells. In vivo occurring Tr1-like cells expressed Eomes, and were clearly distinct from all other CD4+ T-cell subsets, including conventional cytotoxic CD4+ T cells. They expressed Granzyme (Gzm) K, but had lost CD40L and IL-7R expression. Eomes antagonized the Th17 fate, and directly controlled IFN-γ and GzmK expression. However, Eomes binding to the IL-10 promoter was not detectable in human CD4+ T cells, presumably because critical Tbox binding sites of the mouse were not conserved. A precommitment to a Tr1-like fate, i.e. concominant induction of Eomes, GzmK, and IFN-γ, was promoted by IL-4 and IL-12-secreting myeloid dendritic cells. Consistently, Th1 effector memory cells contained precommitted Eomes+ GzmK+ T cells. Stimulation with T-cell receptor (TCR) agonists and IL-27 promoted the generation of Tr1-like effector cells by inducing switching from CD40L to IL-10. Importantly, CD4+ Eomes+ T-cell subsets were present in lymphoid and nonlymphoid tissues, and their frequencies varied systemically in patients with inflammatory bowel disease and graft-versus-host disease. We propose that Eomes+ Tr1-like cells are effector cells of a unique GzmK-expressing CD4+ T-cell subset.
| Original language | English |
|---|---|
| Number of pages | 16 |
| Journal | European Journal of Immunology |
| DOIs | |
| Publication status | E-pub ahead of print - Nov 15 2018 |
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Eomesodermin controls a unique differentiation program in human IL-10 and IFN-γ coproducing regulatory T cells. / Gruarin, Paola; Maglie, Stefano; De Simone, Marco; Häringer, Barbara; Vasco, Chiara; Ranzani, Valeria; Bosotti, Roberto; Noddings, Johanna S; Larghi, Paola; Facciotti, Federica; Sarnicola, Maria L; Martinovic, Martina; Crosti, Mariacristina; Moro, Monica; Rossi, Riccardo L; Bernardo, Maria E; Caprioli, Flavio; Locatelli, Franco; Rossetti, Grazisa; Abrignani, Sergio; Pagani, Massimiliano; Geginat, Jens.
In: European Journal of Immunology, 15.11.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Eomesodermin controls a unique differentiation program in human IL-10 and IFN-γ coproducing regulatory T cells
AU - Gruarin, Paola
AU - Maglie, Stefano
AU - De Simone, Marco
AU - Häringer, Barbara
AU - Vasco, Chiara
AU - Ranzani, Valeria
AU - Bosotti, Roberto
AU - Noddings, Johanna S
AU - Larghi, Paola
AU - Facciotti, Federica
AU - Sarnicola, Maria L
AU - Martinovic, Martina
AU - Crosti, Mariacristina
AU - Moro, Monica
AU - Rossi, Riccardo L
AU - Bernardo, Maria E
AU - Caprioli, Flavio
AU - Locatelli, Franco
AU - Rossetti, Grazisa
AU - Abrignani, Sergio
AU - Pagani, Massimiliano
AU - Geginat, Jens
N1 - © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2018/11/15
Y1 - 2018/11/15
N2 - Whether human IL-10-producing regulatory T cells ("Tr1") represent a distinct differentiation lineage or an unstable activation stage remains a key unsolved issue. Here, we report that Eomesodermin (Eomes) acted as a lineage-defining transcription factor in human IFN-γ/IL-10 coproducing Tr1-like cells. In vivo occurring Tr1-like cells expressed Eomes, and were clearly distinct from all other CD4+ T-cell subsets, including conventional cytotoxic CD4+ T cells. They expressed Granzyme (Gzm) K, but had lost CD40L and IL-7R expression. Eomes antagonized the Th17 fate, and directly controlled IFN-γ and GzmK expression. However, Eomes binding to the IL-10 promoter was not detectable in human CD4+ T cells, presumably because critical Tbox binding sites of the mouse were not conserved. A precommitment to a Tr1-like fate, i.e. concominant induction of Eomes, GzmK, and IFN-γ, was promoted by IL-4 and IL-12-secreting myeloid dendritic cells. Consistently, Th1 effector memory cells contained precommitted Eomes+ GzmK+ T cells. Stimulation with T-cell receptor (TCR) agonists and IL-27 promoted the generation of Tr1-like effector cells by inducing switching from CD40L to IL-10. Importantly, CD4+ Eomes+ T-cell subsets were present in lymphoid and nonlymphoid tissues, and their frequencies varied systemically in patients with inflammatory bowel disease and graft-versus-host disease. We propose that Eomes+ Tr1-like cells are effector cells of a unique GzmK-expressing CD4+ T-cell subset.
AB - Whether human IL-10-producing regulatory T cells ("Tr1") represent a distinct differentiation lineage or an unstable activation stage remains a key unsolved issue. Here, we report that Eomesodermin (Eomes) acted as a lineage-defining transcription factor in human IFN-γ/IL-10 coproducing Tr1-like cells. In vivo occurring Tr1-like cells expressed Eomes, and were clearly distinct from all other CD4+ T-cell subsets, including conventional cytotoxic CD4+ T cells. They expressed Granzyme (Gzm) K, but had lost CD40L and IL-7R expression. Eomes antagonized the Th17 fate, and directly controlled IFN-γ and GzmK expression. However, Eomes binding to the IL-10 promoter was not detectable in human CD4+ T cells, presumably because critical Tbox binding sites of the mouse were not conserved. A precommitment to a Tr1-like fate, i.e. concominant induction of Eomes, GzmK, and IFN-γ, was promoted by IL-4 and IL-12-secreting myeloid dendritic cells. Consistently, Th1 effector memory cells contained precommitted Eomes+ GzmK+ T cells. Stimulation with T-cell receptor (TCR) agonists and IL-27 promoted the generation of Tr1-like effector cells by inducing switching from CD40L to IL-10. Importantly, CD4+ Eomes+ T-cell subsets were present in lymphoid and nonlymphoid tissues, and their frequencies varied systemically in patients with inflammatory bowel disease and graft-versus-host disease. We propose that Eomes+ Tr1-like cells are effector cells of a unique GzmK-expressing CD4+ T-cell subset.
U2 - 10.1002/eji.201847722
DO - 10.1002/eji.201847722
M3 - Article
C2 - 30431161
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
ER -