Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 457-466 |
| Number of pages | 10 |
| Journal | European journal of dermatology : EJD |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2018 |
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Keywords
- angiogenic growth factor
- eosin
- leucocyte
- psoriasis
Cite this
Eosin treatment for psoriasis reduces skin leukocyte infiltration and secretion of inflammatory chemokines and angiogenic factors. / Capriotti, L.; Didona, B.; Madonna, S.; Scarponi, C.; Pilla, M.A.; Facchiano, F.; Cordella, M.; Cavani, A.; Failla, C.M.
In: European journal of dermatology : EJD, Vol. 28, No. 4, 2018, p. 457-466.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Eosin treatment for psoriasis reduces skin leukocyte infiltration and secretion of inflammatory chemokines and angiogenic factors
AU - Capriotti, L.
AU - Didona, B.
AU - Madonna, S.
AU - Scarponi, C.
AU - Pilla, M.A.
AU - Facchiano, F.
AU - Cordella, M.
AU - Cavani, A.
AU - Failla, C.M.
N1 - Export Date: 11 April 2019
PY - 2018
Y1 - 2018
N2 - BACKGROUND: Eosin has been traditionally employed as a topical treatment for psoriasis, but the biological mechanism of its therapeutic action has not been fully elucidated. OBJECTIVES: To analyse eosin effects on psoriatic skin in vivo and keratinocytes and endothelial cells in vitro. MATERIALS & METHODS: Skin biopsies were taken from psoriatic plaques before and after a three-day eosin treatment and processed for histological analysis. Cultured human psoriatic keratinocytes and dermal endothelial cells were treated with eosin, and release of inflammatory chemokines was analysed by multiplexed bead-based immunoassay and ELISA. RESULTS: In patients, the three-day eosin treatment significantly reduced the number of infiltrating T lymphocytes, neutrophilic granulocytes, and dermal dendritic cells. A reduction in VEGF-A expression was also observed. In vitro, eosin treatment significantly decreased the release of CCL2, CCL5, and VEGF-A by keratinocytes and angiopoietin-2 by endothelial cells. CONCLUSIONS: Eosin treatment impacts on psoriatic inflammatory infiltrates and dampens the release of proinflammatory chemokines and angiogenic factors.
AB - BACKGROUND: Eosin has been traditionally employed as a topical treatment for psoriasis, but the biological mechanism of its therapeutic action has not been fully elucidated. OBJECTIVES: To analyse eosin effects on psoriatic skin in vivo and keratinocytes and endothelial cells in vitro. MATERIALS & METHODS: Skin biopsies were taken from psoriatic plaques before and after a three-day eosin treatment and processed for histological analysis. Cultured human psoriatic keratinocytes and dermal endothelial cells were treated with eosin, and release of inflammatory chemokines was analysed by multiplexed bead-based immunoassay and ELISA. RESULTS: In patients, the three-day eosin treatment significantly reduced the number of infiltrating T lymphocytes, neutrophilic granulocytes, and dermal dendritic cells. A reduction in VEGF-A expression was also observed. In vitro, eosin treatment significantly decreased the release of CCL2, CCL5, and VEGF-A by keratinocytes and angiopoietin-2 by endothelial cells. CONCLUSIONS: Eosin treatment impacts on psoriatic inflammatory infiltrates and dampens the release of proinflammatory chemokines and angiogenic factors.
KW - angiogenic growth factor
KW - eosin
KW - leucocyte
KW - psoriasis
U2 - 10.1684/ejd.2018.3357
DO - 10.1684/ejd.2018.3357
M3 - Article
VL - 28
SP - 457
EP - 466
JO - European Journal of Dermatology
JF - European Journal of Dermatology
SN - 1167-1122
IS - 4
ER -