Eosin treatment for psoriasis reduces skin leukocyte infiltration and secretion of inflammatory chemokines and angiogenic factors

L. Capriotti, B. Didona, S. Madonna, C. Scarponi, M.A. Pilla, F. Facchiano, M. Cordella, A. Cavani, C.M. Failla

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Eosin has been traditionally employed as a topical treatment for psoriasis, but the biological mechanism of its therapeutic action has not been fully elucidated. OBJECTIVES: To analyse eosin effects on psoriatic skin in vivo and keratinocytes and endothelial cells in vitro. MATERIALS & METHODS: Skin biopsies were taken from psoriatic plaques before and after a three-day eosin treatment and processed for histological analysis. Cultured human psoriatic keratinocytes and dermal endothelial cells were treated with eosin, and release of inflammatory chemokines was analysed by multiplexed bead-based immunoassay and ELISA. RESULTS: In patients, the three-day eosin treatment significantly reduced the number of infiltrating T lymphocytes, neutrophilic granulocytes, and dermal dendritic cells. A reduction in VEGF-A expression was also observed. In vitro, eosin treatment significantly decreased the release of CCL2, CCL5, and VEGF-A by keratinocytes and angiopoietin-2 by endothelial cells. CONCLUSIONS: Eosin treatment impacts on psoriatic inflammatory infiltrates and dampens the release of proinflammatory chemokines and angiogenic factors.
Original languageEnglish
Pages (from-to)457-466
Number of pages10
JournalEuropean journal of dermatology : EJD
Volume28
Issue number4
DOIs
Publication statusPublished - 2018

Fingerprint

Angiogenesis Inducing Agents
Eosine Yellowish-(YS)
Chemokines
Psoriasis
Leukocytes
Skin
Keratinocytes
Endothelial Cells
Vascular Endothelial Growth Factor A
Therapeutics
Angiopoietin-2
Langerhans Cells
Immunoassay
Granulocytes
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Biopsy

Keywords

  • angiogenic growth factor
  • eosin
  • leucocyte
  • psoriasis

Cite this

Eosin treatment for psoriasis reduces skin leukocyte infiltration and secretion of inflammatory chemokines and angiogenic factors. / Capriotti, L.; Didona, B.; Madonna, S.; Scarponi, C.; Pilla, M.A.; Facchiano, F.; Cordella, M.; Cavani, A.; Failla, C.M.

In: European journal of dermatology : EJD, Vol. 28, No. 4, 2018, p. 457-466.

Research output: Contribution to journalArticle

Capriotti, L. ; Didona, B. ; Madonna, S. ; Scarponi, C. ; Pilla, M.A. ; Facchiano, F. ; Cordella, M. ; Cavani, A. ; Failla, C.M. / Eosin treatment for psoriasis reduces skin leukocyte infiltration and secretion of inflammatory chemokines and angiogenic factors. In: European journal of dermatology : EJD. 2018 ; Vol. 28, No. 4. pp. 457-466.
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abstract = "BACKGROUND: Eosin has been traditionally employed as a topical treatment for psoriasis, but the biological mechanism of its therapeutic action has not been fully elucidated. OBJECTIVES: To analyse eosin effects on psoriatic skin in vivo and keratinocytes and endothelial cells in vitro. MATERIALS & METHODS: Skin biopsies were taken from psoriatic plaques before and after a three-day eosin treatment and processed for histological analysis. Cultured human psoriatic keratinocytes and dermal endothelial cells were treated with eosin, and release of inflammatory chemokines was analysed by multiplexed bead-based immunoassay and ELISA. RESULTS: In patients, the three-day eosin treatment significantly reduced the number of infiltrating T lymphocytes, neutrophilic granulocytes, and dermal dendritic cells. A reduction in VEGF-A expression was also observed. In vitro, eosin treatment significantly decreased the release of CCL2, CCL5, and VEGF-A by keratinocytes and angiopoietin-2 by endothelial cells. CONCLUSIONS: Eosin treatment impacts on psoriatic inflammatory infiltrates and dampens the release of proinflammatory chemokines and angiogenic factors.",
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T1 - Eosin treatment for psoriasis reduces skin leukocyte infiltration and secretion of inflammatory chemokines and angiogenic factors

AU - Capriotti, L.

AU - Didona, B.

AU - Madonna, S.

AU - Scarponi, C.

AU - Pilla, M.A.

AU - Facchiano, F.

AU - Cordella, M.

AU - Cavani, A.

AU - Failla, C.M.

N1 - Export Date: 11 April 2019

PY - 2018

Y1 - 2018

N2 - BACKGROUND: Eosin has been traditionally employed as a topical treatment for psoriasis, but the biological mechanism of its therapeutic action has not been fully elucidated. OBJECTIVES: To analyse eosin effects on psoriatic skin in vivo and keratinocytes and endothelial cells in vitro. MATERIALS & METHODS: Skin biopsies were taken from psoriatic plaques before and after a three-day eosin treatment and processed for histological analysis. Cultured human psoriatic keratinocytes and dermal endothelial cells were treated with eosin, and release of inflammatory chemokines was analysed by multiplexed bead-based immunoassay and ELISA. RESULTS: In patients, the three-day eosin treatment significantly reduced the number of infiltrating T lymphocytes, neutrophilic granulocytes, and dermal dendritic cells. A reduction in VEGF-A expression was also observed. In vitro, eosin treatment significantly decreased the release of CCL2, CCL5, and VEGF-A by keratinocytes and angiopoietin-2 by endothelial cells. CONCLUSIONS: Eosin treatment impacts on psoriatic inflammatory infiltrates and dampens the release of proinflammatory chemokines and angiogenic factors.

AB - BACKGROUND: Eosin has been traditionally employed as a topical treatment for psoriasis, but the biological mechanism of its therapeutic action has not been fully elucidated. OBJECTIVES: To analyse eosin effects on psoriatic skin in vivo and keratinocytes and endothelial cells in vitro. MATERIALS & METHODS: Skin biopsies were taken from psoriatic plaques before and after a three-day eosin treatment and processed for histological analysis. Cultured human psoriatic keratinocytes and dermal endothelial cells were treated with eosin, and release of inflammatory chemokines was analysed by multiplexed bead-based immunoassay and ELISA. RESULTS: In patients, the three-day eosin treatment significantly reduced the number of infiltrating T lymphocytes, neutrophilic granulocytes, and dermal dendritic cells. A reduction in VEGF-A expression was also observed. In vitro, eosin treatment significantly decreased the release of CCL2, CCL5, and VEGF-A by keratinocytes and angiopoietin-2 by endothelial cells. CONCLUSIONS: Eosin treatment impacts on psoriatic inflammatory infiltrates and dampens the release of proinflammatory chemokines and angiogenic factors.

KW - angiogenic growth factor

KW - eosin

KW - leucocyte

KW - psoriasis

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DO - 10.1684/ejd.2018.3357

M3 - Article

VL - 28

SP - 457

EP - 466

JO - European Journal of Dermatology

JF - European Journal of Dermatology

SN - 1167-1122

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ER -