EP2 receptor stimulation promotes calcium responses in astrocytes via activation of the adenylyl cyclase pathway

A. Di Cesare, P. Del Piccolo, D. Zacchetti, F. Grohovaz

Research output: Contribution to journalArticle

Abstract

Astrocytes are a heterogeneous population of cells that are endowed with a great variety of receptors for neurotransmitters and neuromodulators. Recently prostaglandin E2 has attracted great interest since it is not only released by astrocytes but also activates receptors coupled to either phospholipase C or adenylyl cyclase. We report that EP2 receptor stimulation triggers cAMP production but also causes release of Ca2+ from intracellular stores. This effect is shared by other receptors similarly coupled to adenylyl cyclase and elicited by direct stimulation of the enzyme or application of cAMP analogues. However, the stimulation of the Ca2+ response by cAMP is not mediated by protein kinase A, since a specific antagonist of this kinase had no effect. Such a cross-talk between cAMP and Ca2+ was not observed in all astrocytes. It might therefore reflect a specific resource of either a subpopulation or astrocytes in a specific functional state.

Original languageEnglish
Pages (from-to)2546-2553
Number of pages8
JournalCellular and Molecular Life Sciences
Volume63
Issue number21
DOIs
Publication statusPublished - Nov 2006

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Keywords

  • Butaprost
  • cAMP
  • Forskolin
  • Isoproterenol
  • Prostaglandin E
  • Sulprostone

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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