Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube

Andrew D. Bergemann, Lee Zhang, Ming Ko Chiang, Riccardo Brambilla, Rüdiger Klein, John G. Flanagan

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The ephrins are a family of ligands that bind to Eph family receptor tyrosine kinases, and have been implicated in axon guidance and other patterning processes during vertebrate development. We describe here the identification and characterization of murine ephrin-B3. The cDNA encodes a 340 amino acid transmembrane molecule, most closely related to the two other known transmembrane ligands, ephrin-B1 and ephrin-B2. In addition to homology in their extracellular receptor binding domains, these transmembrane ligands share striking homology between their cytoplasmic domains, with 31 of the last 34 amino acids of ephrin-B3 being identical to ephrin-B2, suggesting functional interactions of the cytoplasmic tail. While most Eph family ligands are promiscuous in their interactions with Eph receptors, binding studies with the five receptors known to bind other transmembrane ligands only revealed a high affinity interaction of ephrin-B3 with EphB3, with a dissociation constant of approximately 1 nM. In situ hybridization of mouse embryos showed ephrin-B3 is expressed prominently at the dorsal and ventral midline of the neural tube, particularly in the floor plate, a structure with key functions in patterning the nervous system. The isolation of this ligand may help to elucidate the molecular basis of patterning activities at the neural tube midline.

Original languageEnglish
Pages (from-to)471-480
Number of pages10
JournalOncogene
Volume16
Issue number4
Publication statusPublished - Jan 29 1998

Fingerprint

EphB3 Receptor
Ephrin-B3
Neural Tube
Eph Family Receptors
Ligands
Ephrin-B2
Ephrin-B1
Ephrins
Amino Acids
Nervous System
In Situ Hybridization
Vertebrates
Tail
Embryonic Structures
Complementary DNA

Keywords

  • Development
  • Eph
  • Ephrin
  • Kinase
  • Neural
  • Receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Bergemann, A. D., Zhang, L., Chiang, M. K., Brambilla, R., Klein, R., & Flanagan, J. G. (1998). Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene, 16(4), 471-480.

Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. / Bergemann, Andrew D.; Zhang, Lee; Chiang, Ming Ko; Brambilla, Riccardo; Klein, Rüdiger; Flanagan, John G.

In: Oncogene, Vol. 16, No. 4, 29.01.1998, p. 471-480.

Research output: Contribution to journalArticle

Bergemann, AD, Zhang, L, Chiang, MK, Brambilla, R, Klein, R & Flanagan, JG 1998, 'Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube', Oncogene, vol. 16, no. 4, pp. 471-480.
Bergemann AD, Zhang L, Chiang MK, Brambilla R, Klein R, Flanagan JG. Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 1998 Jan 29;16(4):471-480.
Bergemann, Andrew D. ; Zhang, Lee ; Chiang, Ming Ko ; Brambilla, Riccardo ; Klein, Rüdiger ; Flanagan, John G. / Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. In: Oncogene. 1998 ; Vol. 16, No. 4. pp. 471-480.
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