Epidemiological aspects of human rotavirus infection in children hospitalized with acute gastroenteritis in an area of northern Italy

Maria Cristina Medici, Monica Martinelli, Maria Cristina Arcangeletti, Federica Pinardi, Flora De Conto, Icilio Dodi, Raffaele Virdis, Laura Anna Abelli, Annalisa Aloisi, Laura Zerbini, Pierpaolo Valcavi, Adriana Calderaro, Sergio Bernasconi, Gian Carlo Izz, Giuseppe Dettori, Carlo Chezzi

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29 Citations (Scopus)

Abstract

Human rotavirus (HRV) is recognized as the most common cause of severe gastroenteritis in children under 5 years of age. Due to the lack of recent reports about the surveillance of HRV infection in Italy, in this study we assessed the prevalence rate of HRV infection on 1,340 stool samples belonging to 1,264 pediatric patients hospitalized with acute gastroenteritis in the period January 2000-December 2002. The stool samples were submitted to virological investigations by electron microscopy (EM) and conventional cell culture, as well as from January 2002 by RT-PCR for norovirus detection. Reovirus-like particles observed by EM were identified by electropherotyping. Single HRV infections were detected in 302 cases (23.9%, ranging from 19.1% in 2000 to 30.2% in 2001). Mixed infections were observed in 28 cases in which HRV was found to be associated with adenovirus in 16 cases (1.3%), with picornavirus in 4 (0.3%), and with norovirus in 8 (2.1% of the 388 cases examined in 2002). The 3 major epidemic periods of HRV infections were March-May 2000 (66 cases), December 2000-May 2001 (128 cases) and September 2001-April 2002 (105 cases) with peaks in March, January and March, and January, respectively. In the periods of major incidence, single HRV infection accounted even for 52.5% of the gastroenteritis cases monthly examined. According to age distribution, 68.9% (208 cases) of HRV infected children was under 4 years (69.6%:230/330 cases, including mixed infections) and 16.9% (51 cases) was in the 5-12-year age group. The epidemiological aspects of HRV infection, also compared to other enteric virus infections, will contribute to assess the magnitude of the problem of HRV in different settings and to devise strategies for intervention.

Original languageEnglish
JournalActa Biomedica
Volume75
Issue number2
Publication statusPublished - 2004

Fingerprint

Rotavirus Infections
Hospitalized Child
Gastroenteritis
Italy
Rotavirus
Norovirus
Coinfection
Electron Microscopy
Enterovirus Infections
Picornaviridae
Age Distribution
Adenoviridae
Age Groups
Cell Culture Techniques
Cross-Sectional Studies
Pediatrics

Keywords

  • Acute gastroenteritis
  • Epidemiology
  • Hospitalized children
  • Human rotavirus

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Medici, M. C., Martinelli, M., Arcangeletti, M. C., Pinardi, F., De Conto, F., Dodi, I., ... Chezzi, C. (2004). Epidemiological aspects of human rotavirus infection in children hospitalized with acute gastroenteritis in an area of northern Italy. Acta Biomedica, 75(2).

Epidemiological aspects of human rotavirus infection in children hospitalized with acute gastroenteritis in an area of northern Italy. / Medici, Maria Cristina; Martinelli, Monica; Arcangeletti, Maria Cristina; Pinardi, Federica; De Conto, Flora; Dodi, Icilio; Virdis, Raffaele; Abelli, Laura Anna; Aloisi, Annalisa; Zerbini, Laura; Valcavi, Pierpaolo; Calderaro, Adriana; Bernasconi, Sergio; Izz, Gian Carlo; Dettori, Giuseppe; Chezzi, Carlo.

In: Acta Biomedica, Vol. 75, No. 2, 2004.

Research output: Contribution to journalArticle

Medici, MC, Martinelli, M, Arcangeletti, MC, Pinardi, F, De Conto, F, Dodi, I, Virdis, R, Abelli, LA, Aloisi, A, Zerbini, L, Valcavi, P, Calderaro, A, Bernasconi, S, Izz, GC, Dettori, G & Chezzi, C 2004, 'Epidemiological aspects of human rotavirus infection in children hospitalized with acute gastroenteritis in an area of northern Italy', Acta Biomedica, vol. 75, no. 2.
Medici, Maria Cristina ; Martinelli, Monica ; Arcangeletti, Maria Cristina ; Pinardi, Federica ; De Conto, Flora ; Dodi, Icilio ; Virdis, Raffaele ; Abelli, Laura Anna ; Aloisi, Annalisa ; Zerbini, Laura ; Valcavi, Pierpaolo ; Calderaro, Adriana ; Bernasconi, Sergio ; Izz, Gian Carlo ; Dettori, Giuseppe ; Chezzi, Carlo. / Epidemiological aspects of human rotavirus infection in children hospitalized with acute gastroenteritis in an area of northern Italy. In: Acta Biomedica. 2004 ; Vol. 75, No. 2.
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abstract = "Human rotavirus (HRV) is recognized as the most common cause of severe gastroenteritis in children under 5 years of age. Due to the lack of recent reports about the surveillance of HRV infection in Italy, in this study we assessed the prevalence rate of HRV infection on 1,340 stool samples belonging to 1,264 pediatric patients hospitalized with acute gastroenteritis in the period January 2000-December 2002. The stool samples were submitted to virological investigations by electron microscopy (EM) and conventional cell culture, as well as from January 2002 by RT-PCR for norovirus detection. Reovirus-like particles observed by EM were identified by electropherotyping. Single HRV infections were detected in 302 cases (23.9{\%}, ranging from 19.1{\%} in 2000 to 30.2{\%} in 2001). Mixed infections were observed in 28 cases in which HRV was found to be associated with adenovirus in 16 cases (1.3{\%}), with picornavirus in 4 (0.3{\%}), and with norovirus in 8 (2.1{\%} of the 388 cases examined in 2002). The 3 major epidemic periods of HRV infections were March-May 2000 (66 cases), December 2000-May 2001 (128 cases) and September 2001-April 2002 (105 cases) with peaks in March, January and March, and January, respectively. In the periods of major incidence, single HRV infection accounted even for 52.5{\%} of the gastroenteritis cases monthly examined. According to age distribution, 68.9{\%} (208 cases) of HRV infected children was under 4 years (69.6{\%}:230/330 cases, including mixed infections) and 16.9{\%} (51 cases) was in the 5-12-year age group. The epidemiological aspects of HRV infection, also compared to other enteric virus infections, will contribute to assess the magnitude of the problem of HRV in different settings and to devise strategies for intervention.",
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AU - Dodi, Icilio

AU - Virdis, Raffaele

AU - Abelli, Laura Anna

AU - Aloisi, Annalisa

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AU - Valcavi, Pierpaolo

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