Epidemiology of Staphylococcus aureus in Italy: First nationwide survey, 2012

Floriana Campanile, Dafne Bongiorno, Marianna Perez, Gino Mongelli, Laura Sessa, Sabrina Benvenuto, Floriana Gona, Pietro E. Varaldo, Stefania Stefani

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A 3-month epidemiological study to determine the prevalence and antibiotic resistance of Staphylococcus aureus nosocomial infections was performed in 52 centres throughout Italy in 2012. A total of 21,873 pathogens were analysed. The prevalence of S. aureus among all nosocomial pathogens isolated in that period was 11.6% (n = 2541), whilst the prevalence of methicillin-resistant S. aureus (MRSA) among the S. aureus was 35.8% (n = 910). All tested antimicrobials demonstrated 92.2% susceptibility against methicillin-susceptible S. aureus, with the exception of clindamycin (89.7%) and erythromycin (84.2%). Among MRSA, percentages of resistance ranged from 12.6% to >39% for tetracycline, rifampicin, clindamycin and gentamicin; higher percentages were found for erythromycin (65.4%) and fluoroquinolones (72.3-85.8%). Overall, the glycopeptide minimum inhibitory concentration (MIC) distribution showed that 58.3% of strains possessed MICs of 1-2 mg/L and few strains were linezolid- or daptomycin-resistant. Molecular characterisation was performed on 102 MRSA selected from Northern, Central and Southern regions. Five major clones were found: Italian/ST228-I (t001-t023-t041-t1686-t3217), 33.3%; USA500/ST8-IV (t008), 17.6%; E-MRSA15/ST22-IVh (t020-t025-t032-t223), 16.7%; USA100/ST5-II (t002-t653-t1349-t2164-t3217-t388), 14.7%; and Brazilian/ST239/241-III (t030-t037), 3.9%. Five PVL-positive CA-MRSA isolates, belonging to USA300 and minor clones, were also identified. In conclusion, this first nationwide surveillance study showed that in Italy, S. aureus infections accounted for 11.6% of all nosocomial infections; MRSA accounted for approximately one-third of the S. aureus isolates and these were multidrug-resistant organisms. Five major MRSA epidemic clones were observed and were inter-regionally distributed, with ST228-SCCmecI becoming predominant.

Original languageEnglish
Pages (from-to)247-254
Number of pages8
JournalJournal of Global Antimicrobial Resistance
Volume3
Issue number4
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Italy
Staphylococcus aureus
Epidemiology
Linezolid
Clindamycin
Clone Cells
Erythromycin
Cross Infection
Daptomycin
Methicillin
Glycopeptides
Fluoroquinolones
Microbial Sensitivity Tests
Rifampin
Microbial Drug Resistance
Gentamicins
Tetracycline
Surveys and Questionnaires
Epidemiologic Studies

Keywords

  • Antimicrobial resistance
  • Italy
  • Molecular epidemiology
  • MRSA
  • Staphylococcus aureus
  • Surveillance

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Campanile, F., Bongiorno, D., Perez, M., Mongelli, G., Sessa, L., Benvenuto, S., ... Stefani, S. (2015). Epidemiology of Staphylococcus aureus in Italy: First nationwide survey, 2012. Journal of Global Antimicrobial Resistance, 3(4), 247-254. https://doi.org/10.1016/j.jgar.2015.06.006

Epidemiology of Staphylococcus aureus in Italy : First nationwide survey, 2012. / Campanile, Floriana; Bongiorno, Dafne; Perez, Marianna; Mongelli, Gino; Sessa, Laura; Benvenuto, Sabrina; Gona, Floriana; Varaldo, Pietro E.; Stefani, Stefania.

In: Journal of Global Antimicrobial Resistance, Vol. 3, No. 4, 01.12.2015, p. 247-254.

Research output: Contribution to journalArticle

Campanile, F, Bongiorno, D, Perez, M, Mongelli, G, Sessa, L, Benvenuto, S, Gona, F, Varaldo, PE & Stefani, S 2015, 'Epidemiology of Staphylococcus aureus in Italy: First nationwide survey, 2012', Journal of Global Antimicrobial Resistance, vol. 3, no. 4, pp. 247-254. https://doi.org/10.1016/j.jgar.2015.06.006
Campanile, Floriana ; Bongiorno, Dafne ; Perez, Marianna ; Mongelli, Gino ; Sessa, Laura ; Benvenuto, Sabrina ; Gona, Floriana ; Varaldo, Pietro E. ; Stefani, Stefania. / Epidemiology of Staphylococcus aureus in Italy : First nationwide survey, 2012. In: Journal of Global Antimicrobial Resistance. 2015 ; Vol. 3, No. 4. pp. 247-254.
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abstract = "A 3-month epidemiological study to determine the prevalence and antibiotic resistance of Staphylococcus aureus nosocomial infections was performed in 52 centres throughout Italy in 2012. A total of 21,873 pathogens were analysed. The prevalence of S. aureus among all nosocomial pathogens isolated in that period was 11.6{\%} (n = 2541), whilst the prevalence of methicillin-resistant S. aureus (MRSA) among the S. aureus was 35.8{\%} (n = 910). All tested antimicrobials demonstrated 92.2{\%} susceptibility against methicillin-susceptible S. aureus, with the exception of clindamycin (89.7{\%}) and erythromycin (84.2{\%}). Among MRSA, percentages of resistance ranged from 12.6{\%} to >39{\%} for tetracycline, rifampicin, clindamycin and gentamicin; higher percentages were found for erythromycin (65.4{\%}) and fluoroquinolones (72.3-85.8{\%}). Overall, the glycopeptide minimum inhibitory concentration (MIC) distribution showed that 58.3{\%} of strains possessed MICs of 1-2 mg/L and few strains were linezolid- or daptomycin-resistant. Molecular characterisation was performed on 102 MRSA selected from Northern, Central and Southern regions. Five major clones were found: Italian/ST228-I (t001-t023-t041-t1686-t3217), 33.3{\%}; USA500/ST8-IV (t008), 17.6{\%}; E-MRSA15/ST22-IVh (t020-t025-t032-t223), 16.7{\%}; USA100/ST5-II (t002-t653-t1349-t2164-t3217-t388), 14.7{\%}; and Brazilian/ST239/241-III (t030-t037), 3.9{\%}. Five PVL-positive CA-MRSA isolates, belonging to USA300 and minor clones, were also identified. In conclusion, this first nationwide surveillance study showed that in Italy, S. aureus infections accounted for 11.6{\%} of all nosocomial infections; MRSA accounted for approximately one-third of the S. aureus isolates and these were multidrug-resistant organisms. Five major MRSA epidemic clones were observed and were inter-regionally distributed, with ST228-SCCmecI becoming predominant.",
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