Epidermal growth factor receptor (EGFR) gene copy number in colorectal adenoma-carcinoma progression

Marcella Flora, Simonetta Piana, Cristina Bassano, Alessandra Bisagni, Loredana De Marco, Alessia Ciarrocchi, Elena Tagliavini, Giorgio Gardini, Ione Tamagnini, Chiara Banzi, Giancarlo Bisagni

Research output: Contribution to journalArticle

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Abstract

Adenomas are the easily identifiable precursors of the vast majority of colorectal cancers. Some of their morphological features, such as dysplasia, are predictive of their biological evolution toward adenocarcinomas. A large body of evidence has demonstrated that the epidermal growth factor receptor (EGFR) signaling pathway is commonly activated in colorectal cancer and EGFR-target therapies have improved the outcome for colorectal cancer patients. Nevertheless, the mechanisms underlying the role of EGFR in the adenoma-carcinoma sequence are not entirely clear. We retrospectively analyzed EGFR gene copy number by fluorescence in situ hybridization (FISH) in paraffin-embedded tissue from 215 patients recruited through a prospective colorectal cancer screening procedure and undergoing surgical colectomy. We observed that in human colorectal carcinogenesis, EGFR copy number increases progressively, from adenomas with high-grade dysplasia to locally advanced adenocarcinomas, through early invasive adenocarcinomas, suggesting that deregulation of EGFR may correlate with the malignant progression.

Original languageEnglish
Pages (from-to)630-635
Number of pages6
JournalCancer genetics
Volume205
Issue number12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

erbB-1 Genes
Gene Dosage
Epidermal Growth Factor Receptor
Adenoma
Colorectal Neoplasms
Adenocarcinoma
Biological Evolution
Colectomy
Fluorescence In Situ Hybridization
Early Detection of Cancer
Paraffin
Carcinogenesis
Carcinoma

Keywords

  • Colorectal cancer
  • EGFR
  • Fluorescence in situ hybridization
  • Gene copy number

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Epidermal growth factor receptor (EGFR) gene copy number in colorectal adenoma-carcinoma progression. / Flora, Marcella; Piana, Simonetta; Bassano, Cristina; Bisagni, Alessandra; De Marco, Loredana; Ciarrocchi, Alessia; Tagliavini, Elena; Gardini, Giorgio; Tamagnini, Ione; Banzi, Chiara; Bisagni, Giancarlo.

In: Cancer genetics, Vol. 205, No. 12, 12.2012, p. 630-635.

Research output: Contribution to journalArticle

Flora, Marcella ; Piana, Simonetta ; Bassano, Cristina ; Bisagni, Alessandra ; De Marco, Loredana ; Ciarrocchi, Alessia ; Tagliavini, Elena ; Gardini, Giorgio ; Tamagnini, Ione ; Banzi, Chiara ; Bisagni, Giancarlo. / Epidermal growth factor receptor (EGFR) gene copy number in colorectal adenoma-carcinoma progression. In: Cancer genetics. 2012 ; Vol. 205, No. 12. pp. 630-635.
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AU - Tagliavini, Elena

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