Epidermal reconstructs: A new tool to study topical and systemic photoprotective molecules

Muriel Cario-André, Stefania Briganti, Mauro Picardo, Osamu Nikaido, Yvon Gall, José Ginestar, Alain Taïeb

Research output: Contribution to journalArticlepeer-review


In this study, we compared the effects of sunscreens and antioxidants on reconstructed epidermis made with or without melanocytes 24 h after UVB, UVA or UVA+B irradiation. For this purpose, we studied sunburn cells and cyclobutane pyrimidine dimer formation, protein and lipid oxidation, catalase and superoxide dismutase activities and vitamin E levels. Topical sunscreens protected against direct cell death and thymine dimer formation whereas their protective effect against protein and lipid oxidation and antioxidant depletion was less marked partly due to the difficulty of spreading the cream. Antioxidant molecules protected against direct cell death and protein oxidation but not against thymine dimer formation. Since topical sunscreens and systemic antioxidant protected the skin differently, we speculate that their association might protect more efficiently against UV-induced damage. This model is relevant to study systemic molecules but is less practical, due to the technical limitations of studying topical molecules.

Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Issue number2-3
Publication statusPublished - Nov 2002


  • Antioxidants
  • Photoprotection
  • Reconstructed epidermis
  • Sunscreens
  • Ultraviolet A+B

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry


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