TY - JOUR
T1 - Epigenetic markers of exposure to polycyclic aromatic hydrocarbons in Mexican brickmakers
T2 - A pilot study
AU - Alegría-Torres, Jorge Alejandro
AU - Barretta, Francesco
AU - Batres-Esquivel, Lilia E.
AU - Carrizales-Yáñez, Leticia
AU - Pérez-Maldonado, Iván N.
AU - Baccarelli, Andrea
AU - Bertazzi, Pier Alberto
PY - 2013/4
Y1 - 2013/4
N2 - A pilot cross-sectional study was carried out in a group of 39 male brick manufacturers in San Luis Potosi, Mexico to identify epigenetic biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs). A questionnaire was used to compile the smoking and drinking habits, clinical history, working time, and socioeconomic characteristics of the participants. 1-Hydroxypyrene (1-OHP) levels were measured from urine samples using high-performance liquid chromatography, and genomic DNA was isolated from blood samples for methylation analysis using pyrosequencing. The mean 1-OHP level was 0.18μgg-1 creatinine (range 0.023-1.11), which was below the expected occupational exposure level. After adjusting for potential confounders, the 1-OHP urine concentration was negatively associated with DNA methylation of the interleukin 12 (β=-1.57; 95% CI: -2.9 to -0.23; p=0.02) and p53 gene promoters (β=-2.7; 95% CI: -5.46-0.06; p=0.055). Suggestive negative associations were also found for the TNF-α gene (β=-3.9; 95% CI:-8.28-0.48; p=0.08) and Alu sequences (β=-0.55; 95% CI:-1.25-0.16; p=0.12). Although the individual exposures to PAHs as estimated by urinary 1-OHP concentrations were low, changes in specific and global DNA methylation were observed.
AB - A pilot cross-sectional study was carried out in a group of 39 male brick manufacturers in San Luis Potosi, Mexico to identify epigenetic biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs). A questionnaire was used to compile the smoking and drinking habits, clinical history, working time, and socioeconomic characteristics of the participants. 1-Hydroxypyrene (1-OHP) levels were measured from urine samples using high-performance liquid chromatography, and genomic DNA was isolated from blood samples for methylation analysis using pyrosequencing. The mean 1-OHP level was 0.18μgg-1 creatinine (range 0.023-1.11), which was below the expected occupational exposure level. After adjusting for potential confounders, the 1-OHP urine concentration was negatively associated with DNA methylation of the interleukin 12 (β=-1.57; 95% CI: -2.9 to -0.23; p=0.02) and p53 gene promoters (β=-2.7; 95% CI: -5.46-0.06; p=0.055). Suggestive negative associations were also found for the TNF-α gene (β=-3.9; 95% CI:-8.28-0.48; p=0.08) and Alu sequences (β=-0.55; 95% CI:-1.25-0.16; p=0.12). Although the individual exposures to PAHs as estimated by urinary 1-OHP concentrations were low, changes in specific and global DNA methylation were observed.
KW - Brickmakers
KW - DNA methylation
KW - Epigenetic markers
KW - Polycyclic aromatic hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=84875050895&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875050895&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2012.11.077
DO - 10.1016/j.chemosphere.2012.11.077
M3 - Article
C2 - 23305747
AN - SCOPUS:84875050895
VL - 91
SP - 475
EP - 480
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 4
ER -