Epigenetic markers of exposure to polycyclic aromatic hydrocarbons in Mexican brickmakers: A pilot study

Jorge Alejandro Alegría-Torres, Francesco Barretta, Lilia E. Batres-Esquivel, Leticia Carrizales-Yáñez, Iván N. Pérez-Maldonado, Andrea Baccarelli, Pier Alberto Bertazzi

Research output: Contribution to journalArticle

Abstract

A pilot cross-sectional study was carried out in a group of 39 male brick manufacturers in San Luis Potosi, Mexico to identify epigenetic biomarkers of exposure to polycyclic aromatic hydrocarbons (PAHs). A questionnaire was used to compile the smoking and drinking habits, clinical history, working time, and socioeconomic characteristics of the participants. 1-Hydroxypyrene (1-OHP) levels were measured from urine samples using high-performance liquid chromatography, and genomic DNA was isolated from blood samples for methylation analysis using pyrosequencing. The mean 1-OHP level was 0.18μgg-1 creatinine (range 0.023-1.11), which was below the expected occupational exposure level. After adjusting for potential confounders, the 1-OHP urine concentration was negatively associated with DNA methylation of the interleukin 12 (β=-1.57; 95% CI: -2.9 to -0.23; p=0.02) and p53 gene promoters (β=-2.7; 95% CI: -5.46-0.06; p=0.055). Suggestive negative associations were also found for the TNF-α gene (β=-3.9; 95% CI:-8.28-0.48; p=0.08) and Alu sequences (β=-0.55; 95% CI:-1.25-0.16; p=0.12). Although the individual exposures to PAHs as estimated by urinary 1-OHP concentrations were low, changes in specific and global DNA methylation were observed.

Original languageEnglish
Pages (from-to)475-480
Number of pages6
JournalChemosphere
Volume91
Issue number4
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Polycyclic Aromatic Hydrocarbons
methylation
DNA Methylation
Polycyclic aromatic hydrocarbons
Epigenomics
PAH
Genes
Urine
DNA
urine
Methylation
p53 Genes
High performance liquid chromatography
Biomarkers
Occupational Exposure
Interleukin-12
Brick
Mexico
Drinking
Habits

Keywords

  • Brickmakers
  • DNA methylation
  • Epigenetic markers
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Environmental Chemistry
  • Chemistry(all)

Cite this

Alegría-Torres, J. A., Barretta, F., Batres-Esquivel, L. E., Carrizales-Yáñez, L., Pérez-Maldonado, I. N., Baccarelli, A., & Bertazzi, P. A. (2013). Epigenetic markers of exposure to polycyclic aromatic hydrocarbons in Mexican brickmakers: A pilot study. Chemosphere, 91(4), 475-480. https://doi.org/10.1016/j.chemosphere.2012.11.077

Epigenetic markers of exposure to polycyclic aromatic hydrocarbons in Mexican brickmakers : A pilot study. / Alegría-Torres, Jorge Alejandro; Barretta, Francesco; Batres-Esquivel, Lilia E.; Carrizales-Yáñez, Leticia; Pérez-Maldonado, Iván N.; Baccarelli, Andrea; Bertazzi, Pier Alberto.

In: Chemosphere, Vol. 91, No. 4, 04.2013, p. 475-480.

Research output: Contribution to journalArticle

Alegría-Torres, JA, Barretta, F, Batres-Esquivel, LE, Carrizales-Yáñez, L, Pérez-Maldonado, IN, Baccarelli, A & Bertazzi, PA 2013, 'Epigenetic markers of exposure to polycyclic aromatic hydrocarbons in Mexican brickmakers: A pilot study', Chemosphere, vol. 91, no. 4, pp. 475-480. https://doi.org/10.1016/j.chemosphere.2012.11.077
Alegría-Torres JA, Barretta F, Batres-Esquivel LE, Carrizales-Yáñez L, Pérez-Maldonado IN, Baccarelli A et al. Epigenetic markers of exposure to polycyclic aromatic hydrocarbons in Mexican brickmakers: A pilot study. Chemosphere. 2013 Apr;91(4):475-480. https://doi.org/10.1016/j.chemosphere.2012.11.077
Alegría-Torres, Jorge Alejandro ; Barretta, Francesco ; Batres-Esquivel, Lilia E. ; Carrizales-Yáñez, Leticia ; Pérez-Maldonado, Iván N. ; Baccarelli, Andrea ; Bertazzi, Pier Alberto. / Epigenetic markers of exposure to polycyclic aromatic hydrocarbons in Mexican brickmakers : A pilot study. In: Chemosphere. 2013 ; Vol. 91, No. 4. pp. 475-480.
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