Abstract
Prognostic molecular markers are urgently needed for allowing to discriminate the clinical course of disease of melanoma patients, which is highly heterogeneous and unpredictable also within a specific clinicopathological stage and substage of disease. Alterations in DNA methylation have been reported to be widely present in cutaneous melanoma, profoundly impacting its biology. In line with this notion, we have identified methylation markers as independent prognostic factors in stage IIIC melanoma patients. In this chapter we describe the measurement of the methylation of the Long Interspersed Nucleotide Element-1 sequences in laser capture microdissected tumor tissues as a prognostic tool in stage III melanoma patients, which could help in achieving a more appropriate and patient-tailored clinical management of cutaneous melanoma.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 481-499 |
| Number of pages | 19 |
| Volume | 1102 |
| DOIs | |
| Publication status | Published - 2014 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1102 |
| ISSN (Print) | 10643745 |
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Keywords
- DNA methylation
- Epigenetic
- Long Interspersed Nucleotide Element-1
- Methylation profiles
- Prognosis
- Repetitive elements
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Epigenetic markers of prognosis in melanoma. / Sigalotti, Luca; Fratta, Elisabetta; Parisi, Giulia; Coral, Sandra; Maio, Michele.
Methods in Molecular Biology. Vol. 1102 2014. p. 481-499 (Methods in Molecular Biology; Vol. 1102).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Epigenetic markers of prognosis in melanoma
AU - Sigalotti, Luca
AU - Fratta, Elisabetta
AU - Parisi, Giulia
AU - Coral, Sandra
AU - Maio, Michele
PY - 2014
Y1 - 2014
N2 - Prognostic molecular markers are urgently needed for allowing to discriminate the clinical course of disease of melanoma patients, which is highly heterogeneous and unpredictable also within a specific clinicopathological stage and substage of disease. Alterations in DNA methylation have been reported to be widely present in cutaneous melanoma, profoundly impacting its biology. In line with this notion, we have identified methylation markers as independent prognostic factors in stage IIIC melanoma patients. In this chapter we describe the measurement of the methylation of the Long Interspersed Nucleotide Element-1 sequences in laser capture microdissected tumor tissues as a prognostic tool in stage III melanoma patients, which could help in achieving a more appropriate and patient-tailored clinical management of cutaneous melanoma.
AB - Prognostic molecular markers are urgently needed for allowing to discriminate the clinical course of disease of melanoma patients, which is highly heterogeneous and unpredictable also within a specific clinicopathological stage and substage of disease. Alterations in DNA methylation have been reported to be widely present in cutaneous melanoma, profoundly impacting its biology. In line with this notion, we have identified methylation markers as independent prognostic factors in stage IIIC melanoma patients. In this chapter we describe the measurement of the methylation of the Long Interspersed Nucleotide Element-1 sequences in laser capture microdissected tumor tissues as a prognostic tool in stage III melanoma patients, which could help in achieving a more appropriate and patient-tailored clinical management of cutaneous melanoma.
KW - DNA methylation
KW - Epigenetic
KW - Long Interspersed Nucleotide Element-1
KW - Methylation profiles
KW - Prognosis
KW - Repetitive elements
UR - http://www.scopus.com/inward/record.url?scp=84891822346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891822346&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-727-3_25
DO - 10.1007/978-1-62703-727-3_25
M3 - Chapter
C2 - 24258994
AN - SCOPUS:84891822346
SN - 9781627037266
VL - 1102
T3 - Methods in Molecular Biology
SP - 481
EP - 499
BT - Methods in Molecular Biology
ER -