Epigenetic markers of prognosis in melanoma

Luca Sigalotti; Elisabetta Fratta; Giulia Parisi; Sandra Coral; Michele Maio

doi:10.1007/978-1-62703-727-3_25

Epigenetic markers of prognosis in melanoma

Luca Sigalotti, Elisabetta Fratta, Giulia Parisi, Sandra Coral, Michele Maio

Centro di Riferimento Oncologico

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Prognostic molecular markers are urgently needed for allowing to discriminate the clinical course of disease of melanoma patients, which is highly heterogeneous and unpredictable also within a specific clinicopathological stage and substage of disease. Alterations in DNA methylation have been reported to be widely present in cutaneous melanoma, profoundly impacting its biology. In line with this notion, we have identified methylation markers as independent prognostic factors in stage IIIC melanoma patients. In this chapter we describe the measurement of the methylation of the Long Interspersed Nucleotide Element-1 sequences in laser capture microdissected tumor tissues as a prognostic tool in stage III melanoma patients, which could help in achieving a more appropriate and patient-tailored clinical management of cutaneous melanoma.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	481-499
Number of pages	19
Volume	1102
DOIs	https://doi.org/10.1007/978-1-62703-727-3_25
Publication status	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1102
ISSN (Print)	10643745

Keywords

DNA methylation
Epigenetic
Long Interspersed Nucleotide Element-1
Methylation profiles
Prognosis
Repetitive elements

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-727-3_25

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Sigalotti, L., Fratta, E., Parisi, G., Coral, S., & Maio, M. (2014). Epigenetic markers of prognosis in melanoma. In Methods in Molecular Biology (Vol. 1102, pp. 481-499). (Methods in Molecular Biology; Vol. 1102). https://doi.org/10.1007/978-1-62703-727-3_25

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abstract = "Prognostic molecular markers are urgently needed for allowing to discriminate the clinical course of disease of melanoma patients, which is highly heterogeneous and unpredictable also within a specific clinicopathological stage and substage of disease. Alterations in DNA methylation have been reported to be widely present in cutaneous melanoma, profoundly impacting its biology. In line with this notion, we have identified methylation markers as independent prognostic factors in stage IIIC melanoma patients. In this chapter we describe the measurement of the methylation of the Long Interspersed Nucleotide Element-1 sequences in laser capture microdissected tumor tissues as a prognostic tool in stage III melanoma patients, which could help in achieving a more appropriate and patient-tailored clinical management of cutaneous melanoma.",

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KW - Repetitive elements

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