Abstract
ΔNp73 is an anti-apoptotic product of the TP73 gene whose function in the immune system has not been extensively studied. We analyzed human tonsil B cell subpopulations physically subdivided into resting or activated fractions and found ΔNp73 gene expression essentially in cells bearing features of activation. Moreover, and accordingly, both these fractions proved to be sensitive to treatment in culture with the polyclonal activator TPA that caused substantial increase in ΔNp73 mRNA and protein expression. We also analyzed the TP73 oncogenic-relevant internal promoter 2 (P2) and identified epigenetics as its major regulatory factor since active DNA and histone configurations strictly correlated with ΔNp73 expression upon activation by agents capable of loosening chromatin compaction. Finally, in line with the known TPA pathway, we found that nuclear proteins could bind a sequence corresponding to a unique AP1 site on promoter 2 selectively in the activated cell fraction. Our results suggest a ΔNp73 function in B cell immunity, indicate epigenetics as master TP73 P2 regulator, and point to AP1 site occupancy as playing an putative mechanistic role in this process.
| Original language | English |
|---|---|
| Pages (from-to) | 408-414 |
| Number of pages | 7 |
| Journal | Molecular Immunology |
| Volume | 48 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 2011 |
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Keywords
- B-cells
- DNA methylation
- Epigenetics
- TP73
ASJC Scopus subject areas
- Molecular Biology
- Immunology
Cite this
Epigenetic mechanisms regulate ΔNP73 promoter function in human tonsil B cells. / Brigati, Claudio; Banelli, Barbara; Casciano, Ida; Di Vinci, Angela; Matis, Serena; Cutrona, Giovanna; Forlani, Alessandra; Allemanni, Giorgio; Romani, Massimo.
In: Molecular Immunology, Vol. 48, No. 4, 01.2011, p. 408-414.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epigenetic mechanisms regulate ΔNP73 promoter function in human tonsil B cells
AU - Brigati, Claudio
AU - Banelli, Barbara
AU - Casciano, Ida
AU - Di Vinci, Angela
AU - Matis, Serena
AU - Cutrona, Giovanna
AU - Forlani, Alessandra
AU - Allemanni, Giorgio
AU - Romani, Massimo
PY - 2011/1
Y1 - 2011/1
N2 - ΔNp73 is an anti-apoptotic product of the TP73 gene whose function in the immune system has not been extensively studied. We analyzed human tonsil B cell subpopulations physically subdivided into resting or activated fractions and found ΔNp73 gene expression essentially in cells bearing features of activation. Moreover, and accordingly, both these fractions proved to be sensitive to treatment in culture with the polyclonal activator TPA that caused substantial increase in ΔNp73 mRNA and protein expression. We also analyzed the TP73 oncogenic-relevant internal promoter 2 (P2) and identified epigenetics as its major regulatory factor since active DNA and histone configurations strictly correlated with ΔNp73 expression upon activation by agents capable of loosening chromatin compaction. Finally, in line with the known TPA pathway, we found that nuclear proteins could bind a sequence corresponding to a unique AP1 site on promoter 2 selectively in the activated cell fraction. Our results suggest a ΔNp73 function in B cell immunity, indicate epigenetics as master TP73 P2 regulator, and point to AP1 site occupancy as playing an putative mechanistic role in this process.
AB - ΔNp73 is an anti-apoptotic product of the TP73 gene whose function in the immune system has not been extensively studied. We analyzed human tonsil B cell subpopulations physically subdivided into resting or activated fractions and found ΔNp73 gene expression essentially in cells bearing features of activation. Moreover, and accordingly, both these fractions proved to be sensitive to treatment in culture with the polyclonal activator TPA that caused substantial increase in ΔNp73 mRNA and protein expression. We also analyzed the TP73 oncogenic-relevant internal promoter 2 (P2) and identified epigenetics as its major regulatory factor since active DNA and histone configurations strictly correlated with ΔNp73 expression upon activation by agents capable of loosening chromatin compaction. Finally, in line with the known TPA pathway, we found that nuclear proteins could bind a sequence corresponding to a unique AP1 site on promoter 2 selectively in the activated cell fraction. Our results suggest a ΔNp73 function in B cell immunity, indicate epigenetics as master TP73 P2 regulator, and point to AP1 site occupancy as playing an putative mechanistic role in this process.
KW - B-cells
KW - DNA methylation
KW - Epigenetics
KW - TP73
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U2 - 10.1016/j.molimm.2010.09.001
DO - 10.1016/j.molimm.2010.09.001
M3 - Article
VL - 48
SP - 408
EP - 414
JO - Molecular Immunology
JF - Molecular Immunology
SN - 0161-5890
IS - 4
ER -