Abstract
Background: MicroRNAs are tiny non-coding small endogenous RNAs that regulate gene expression by translational repression, mRNA cleavage and mRNA inhibition. The aim of this study was to investigate the hypermethylation of miR-34b/c and miR-148a in colorectal cancer, and correlate this data to clinicopathological features. We also aimed to evaluate the hypermethylation of miR-34b/c in faeces specimens as a novel non-invasive faecal-DNA-based screening marker.Methods: The 5-aza-2′-deoxycytidine treatment and methylation-specific PCR were carried out to detect the hypermethylation of miR-34b/c and miR-148a. Results: The miR-34b/c hypermethylation was found in 97.5% (79 out of 82) of primary colorectal tumours, P0.0110. In 75% (21 out of 28) of faecal specimens we found a hypermethylation of miR-34b/c while only in 16% (2 out of 12) of high-grade dysplasia. In addition, miR-148a was found to be hypermethylated in 65% (51 out of 78) of colorectal tumour tissues with no significant correlation to clinicopathological features. However, a trend with female gender and advanced age was found, P0.083. We also observed a trend to lower survival rate in patients with miR-148a hypermethylation with 10-year survival probability: 48 vs 65%, P0.561.Conclusions:These findings show that aberrant hypermethylation of miR-34b/c could be an ideal class of early screening marker, whereas miR-148a could serve as a disease progression follow-up marker.
| Original language | English |
|---|---|
| Pages (from-to) | 1770-1778 |
| Number of pages | 9 |
| Journal | British Journal of Cancer |
| Volume | 104 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - May 24 2011 |
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Keywords
- CRC
- faeces
- methylation
- miR-148a
- miR-34b/c
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Epigenetically silenced miR-34b/c as a novel faecal-based screening marker for colorectal cancer. / Kalimutho, M.; Di Cecilia, S.; Del Vecchio Blanco, G.; Roviello, F.; Sileri, P.; Cretella, M.; Formosa, A.; Corso, G.; Marrelli, D.; Pallone, F.; Federici, G.; Bernardini, S.
In: British Journal of Cancer, Vol. 104, No. 11, 24.05.2011, p. 1770-1778.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epigenetically silenced miR-34b/c as a novel faecal-based screening marker for colorectal cancer
AU - Kalimutho, M.
AU - Di Cecilia, S.
AU - Del Vecchio Blanco, G.
AU - Roviello, F.
AU - Sileri, P.
AU - Cretella, M.
AU - Formosa, A.
AU - Corso, G.
AU - Marrelli, D.
AU - Pallone, F.
AU - Federici, G.
AU - Bernardini, S.
PY - 2011/5/24
Y1 - 2011/5/24
N2 - Background: MicroRNAs are tiny non-coding small endogenous RNAs that regulate gene expression by translational repression, mRNA cleavage and mRNA inhibition. The aim of this study was to investigate the hypermethylation of miR-34b/c and miR-148a in colorectal cancer, and correlate this data to clinicopathological features. We also aimed to evaluate the hypermethylation of miR-34b/c in faeces specimens as a novel non-invasive faecal-DNA-based screening marker.Methods: The 5-aza-2′-deoxycytidine treatment and methylation-specific PCR were carried out to detect the hypermethylation of miR-34b/c and miR-148a. Results: The miR-34b/c hypermethylation was found in 97.5% (79 out of 82) of primary colorectal tumours, P0.0110. In 75% (21 out of 28) of faecal specimens we found a hypermethylation of miR-34b/c while only in 16% (2 out of 12) of high-grade dysplasia. In addition, miR-148a was found to be hypermethylated in 65% (51 out of 78) of colorectal tumour tissues with no significant correlation to clinicopathological features. However, a trend with female gender and advanced age was found, P0.083. We also observed a trend to lower survival rate in patients with miR-148a hypermethylation with 10-year survival probability: 48 vs 65%, P0.561.Conclusions:These findings show that aberrant hypermethylation of miR-34b/c could be an ideal class of early screening marker, whereas miR-148a could serve as a disease progression follow-up marker.
AB - Background: MicroRNAs are tiny non-coding small endogenous RNAs that regulate gene expression by translational repression, mRNA cleavage and mRNA inhibition. The aim of this study was to investigate the hypermethylation of miR-34b/c and miR-148a in colorectal cancer, and correlate this data to clinicopathological features. We also aimed to evaluate the hypermethylation of miR-34b/c in faeces specimens as a novel non-invasive faecal-DNA-based screening marker.Methods: The 5-aza-2′-deoxycytidine treatment and methylation-specific PCR were carried out to detect the hypermethylation of miR-34b/c and miR-148a. Results: The miR-34b/c hypermethylation was found in 97.5% (79 out of 82) of primary colorectal tumours, P0.0110. In 75% (21 out of 28) of faecal specimens we found a hypermethylation of miR-34b/c while only in 16% (2 out of 12) of high-grade dysplasia. In addition, miR-148a was found to be hypermethylated in 65% (51 out of 78) of colorectal tumour tissues with no significant correlation to clinicopathological features. However, a trend with female gender and advanced age was found, P0.083. We also observed a trend to lower survival rate in patients with miR-148a hypermethylation with 10-year survival probability: 48 vs 65%, P0.561.Conclusions:These findings show that aberrant hypermethylation of miR-34b/c could be an ideal class of early screening marker, whereas miR-148a could serve as a disease progression follow-up marker.
KW - CRC
KW - faeces
KW - methylation
KW - miR-148a
KW - miR-34b/c
UR - http://www.scopus.com/inward/record.url?scp=79957603048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79957603048&partnerID=8YFLogxK
U2 - 10.1038/bjc.2011.82
DO - 10.1038/bjc.2011.82
M3 - Article
C2 - 21610744
AN - SCOPUS:79957603048
VL - 104
SP - 1770
EP - 1778
JO - British Journal of Cancer
JF - British Journal of Cancer
SN - 0007-0920
IS - 11
ER -