Epigenome-wide association of DNA methylation markers in peripheral blood from Indian Asians and Europeans with incident type 2 diabetes: A nested case-control study

John C. Chambers, Marie Loh, Benjamin Lehne, Alexander Drong, Jennifer Kriebel, Valeria Motta, Simone Wahl, Hannah R. Elliott, Federica Rota, William R. Scott, Weihua Zhang, Sian Tsung Tan, Gianluca Campanella, Marc Chadeau-Hyam, Loic Yengo, Rebecca C. Richmond, Martyna Adamowicz-Brice, Uzma Afzal, Kiymet Bozaoglu, Zuan Yu MokHong Kiat Ng, François Pattou, Holger Prokisch, Michelle Ann Rozario, Letizia Tarantini, James Abbott, Mika Ala-Korpela, Benedetta Albetti, Ole Ammerpohl, Pier Alberto Bertazzi, Christine Blancher, Robert Caiazzo, John Danesh, Tom R. Gaunt, Simon de Lusignan, Christian Gieger, Thomas Illig, Sujeet Jha, Simon Jones, Jeremy Jowett, Antti J. Kangas, Anuradhani Kasturiratne, Norihiro Kato, Navaratnam Kotea, Sudhir Kowlessur, Janne Pitkäniemi, Prakash Punjabi, Danish Saleheen, Clemens Schafmayer, Pasi Soininen, E. Shyong Tai, Barbara Thorand, Jaakko Tuomilehto, Ananda Rajitha Wickremasinghe, Soterios A. Kyrtopoulos, Timothy J. Aitman, Christian Herder, Jochen Hampe, Stéphane Cauchi, Caroline L. Relton, Philippe Froguel, Richie Soong, Paolo Vineis, Marjo Riitta Jarvelin, James Scott, Harald Grallert, Valentina Bollati, Paul Elliott, Mark I. McCarthy, Jaspal S. Kooner

Research output: Contribution to journalArticle

Abstract

Background: Indian Asians, who make up a quarter of the world's population, are at high risk of developing type 2 diabetes. We investigated whether DNA methylation is associated with future type 2 diabetes incidence in Indian Asians and whether differences in methylation patterns between Indian Asians and Europeans are associated with, and could be used to predict, differences in the magnitude of risk of developing type 2 diabetes. Methods: We did a nested case-control study of DNA methylation in Indian Asians and Europeans with incident type 2 diabetes who were identified from the 8-year follow-up of 25 372 participants in the London Life Sciences Prospective Population (LOLIPOP) study. Patients were recruited between May 1, 2002, and Sept 12, 2008. We did epigenome-wide association analysis using samples from Indian Asians with incident type 2 diabetes and age-matched and sex-matched Indian Asian controls, followed by replication testing of top-ranking signals in Europeans. For both discovery and replication, DNA methylation was measured in the baseline blood sample, which was collected before the onset of type 2 diabetes. Epigenome-wide significance was set at p-7. We compared methylation levels between Indian Asian and European controls without type 2 diabetes at baseline to estimate the potential contribution of DNA methylation to increased risk of future type 2 diabetes incidence among Indian Asians. Findings: 1608 (11·9%) of 13 535 Indian Asians and 306 (4·3%) of 7066 Europeans developed type 2 diabetes over a mean of 8·5 years (SD 1·8) of follow-up. The age-adjusted and sex-adjusted incidence of type 2 diabetes was 3·1 times (95% CI 2·8-3·6; p-17) for ABCG1, 0·94 (0·92-0·95; p=4·2 × 10-11) for PHOSPHO1, 0·94 (0·92-0·96; p=1·4 × 10-9) for SOCS3, 1·07 (1·04-1·09; p=2·1 × 10-10) for SREBF1, and 0·92 (0·90-0·94; p=1·2 × 10-17) for TXNIP. A methylation score combining results for the five loci was associated with future type 2 diabetes incidence (relative risk quartile 4 vs quartile 1 3·51, 95% CI 2·79-4·42; p=1·3 × 10-26), and was independent of established risk factors. Methylation score was higher among Indian Asians than Europeans (p=1 × 10-34). Interpretation: DNA methylation might provide new insights into the pathways underlying type 2 diabetes and offer new opportunities for risk stratification and prevention of type 2 diabetes among Indian Asians. Funding: The European Union, the UK National Institute for Health Research, the Wellcome Trust, the UK Medical Research Council, Action on Hearing Loss, the UK Biotechnology and Biological Sciences Research Council, the Oak Foundation, the Economic and Social Research Council, Helmholtz Zentrum Munchen, the German Research Center for Environmental Health, the German Federal Ministry of Education and Research, the German Center for Diabetes Research, the Munich Center for Health Sciences, the Ministry of Science and Research of the State of North Rhine-Westphalia, and the German Federal Ministry of Health.

Original languageEnglish
Pages (from-to)526-534
Number of pages9
JournalThe Lancet Diabetes and Endocrinology
Volume3
Issue number7
DOIs
Publication statusPublished - Jul 1 2015

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine
  • Endocrinology

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    Chambers, J. C., Loh, M., Lehne, B., Drong, A., Kriebel, J., Motta, V., Wahl, S., Elliott, H. R., Rota, F., Scott, W. R., Zhang, W., Tan, S. T., Campanella, G., Chadeau-Hyam, M., Yengo, L., Richmond, R. C., Adamowicz-Brice, M., Afzal, U., Bozaoglu, K., ... Kooner, J. S. (2015). Epigenome-wide association of DNA methylation markers in peripheral blood from Indian Asians and Europeans with incident type 2 diabetes: A nested case-control study. The Lancet Diabetes and Endocrinology, 3(7), 526-534. https://doi.org/10.1016/S2213-8587(15)00127-8