Epistasis between IL1A, IL1B, TNF, HTR2A, 5-HTTLPR and TPH2 variations does not impact alcohol dependence disorder features

Antonio Drago, Ioannis Liappas, Carmine Petio, Diego Albani, Gianluigi Forloni, Petros Malitas, Christina Piperi, Antonis Politis, Elias O. Tzavellas, Katerina K. Zisaki, Francesca Prato, Sara Batelli, Letizia Polito, Diana De Ronchi, Thomas Paparrigopoulos, Anastasios Kalofoutis, Alessandro Serretti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We assessed a set of biological (HDL, LDL, SGOT,SGPT, GGT, HTc, Hb and T levels) and psychometric variables (investigated through HAM-D, HAM-A, GAS, Liebowitz Social Anxiety Scale, Mark & Mathews Scale, Leyton scale, and Pilowski scale) in a sample of 64 alcohol dependent patients, at baseline and after a detoxification treatment. Moreover, we recruited 47 non-consanguineous relatives who did not suffer alcohol related disorders and underwent the same tests. In both groups we genotyped 11 genetic variations (rs1800587; rs3087258; rs1799724; 5-HTTLPR; rs1386493; rs1386494; rs1487275; rs1843809; rs4570625; rs2129575; rs6313) located in genes whose impact on alcohol related behaviors and disorders has been hypothesized (IL1A, IL1B, TNF, 5-HTTLPR, TPH2 and HTR2A). We analyzed the epistasis of these genetic variations upon the biological and psychological dimensions in the cases and their relatives. Further on, we analyzed the effects of the combined genetic variations on the short - term detoxification treatment efficacy. Finally, being the only not yet investigated variation within this sample, we analyzed the impact of the rs6313 alone on baseline assessment and treatment efficacy. We detected the following results: the couple rs6313 + rs2129575 affected the Leyton -Trait at admission (p = 0.01) (obsessive-compulsive trait), whilst rs1800587 + 5-HTTLPR impacted the Pilowski test at admission (p = 0.01) (hypochondriac symptoms). These results did not survive Bonferroni correction (p ≤ 0.004). This lack of association may depend on the incomplete gene coverage or on the small sample size which limited the power of the study. On the other hand, it may reflect a substantial absence of relevance of the genotype variants toward the alcohol related investigated dimensions. Nonetheless, the marginal significance we detected could witness an informative correlation worth investigating in larger samples.

Original languageEnglish
Pages (from-to)1980-1990
Number of pages11
JournalInternational Journal of Environmental Research and Public Health
Volume6
Issue number7
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Alcohol-Related Disorders
Alcoholism
Alcohols
Aspartate Aminotransferases
Alanine Transaminase
Psychometrics
Mental Disorders
Sample Size
Genes
Anxiety
Genotype
Psychology
Therapeutics

Keywords

  • 5HTPR
  • Alcohol dependence
  • Association
  • Gene
  • HTR2A
  • IL1A
  • IL1B
  • TNF
  • TPH2

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Epistasis between IL1A, IL1B, TNF, HTR2A, 5-HTTLPR and TPH2 variations does not impact alcohol dependence disorder features. / Drago, Antonio; Liappas, Ioannis; Petio, Carmine; Albani, Diego; Forloni, Gianluigi; Malitas, Petros; Piperi, Christina; Politis, Antonis; Tzavellas, Elias O.; Zisaki, Katerina K.; Prato, Francesca; Batelli, Sara; Polito, Letizia; De Ronchi, Diana; Paparrigopoulos, Thomas; Kalofoutis, Anastasios; Serretti, Alessandro.

In: International Journal of Environmental Research and Public Health, Vol. 6, No. 7, 07.2009, p. 1980-1990.

Research output: Contribution to journalArticle

Drago, A, Liappas, I, Petio, C, Albani, D, Forloni, G, Malitas, P, Piperi, C, Politis, A, Tzavellas, EO, Zisaki, KK, Prato, F, Batelli, S, Polito, L, De Ronchi, D, Paparrigopoulos, T, Kalofoutis, A & Serretti, A 2009, 'Epistasis between IL1A, IL1B, TNF, HTR2A, 5-HTTLPR and TPH2 variations does not impact alcohol dependence disorder features', International Journal of Environmental Research and Public Health, vol. 6, no. 7, pp. 1980-1990. https://doi.org/10.3390/ijerph6071980
Drago, Antonio ; Liappas, Ioannis ; Petio, Carmine ; Albani, Diego ; Forloni, Gianluigi ; Malitas, Petros ; Piperi, Christina ; Politis, Antonis ; Tzavellas, Elias O. ; Zisaki, Katerina K. ; Prato, Francesca ; Batelli, Sara ; Polito, Letizia ; De Ronchi, Diana ; Paparrigopoulos, Thomas ; Kalofoutis, Anastasios ; Serretti, Alessandro. / Epistasis between IL1A, IL1B, TNF, HTR2A, 5-HTTLPR and TPH2 variations does not impact alcohol dependence disorder features. In: International Journal of Environmental Research and Public Health. 2009 ; Vol. 6, No. 7. pp. 1980-1990.
@article{6ad205b71e7b4936aa267b0d1184db91,
title = "Epistasis between IL1A, IL1B, TNF, HTR2A, 5-HTTLPR and TPH2 variations does not impact alcohol dependence disorder features",
abstract = "We assessed a set of biological (HDL, LDL, SGOT,SGPT, GGT, HTc, Hb and T levels) and psychometric variables (investigated through HAM-D, HAM-A, GAS, Liebowitz Social Anxiety Scale, Mark & Mathews Scale, Leyton scale, and Pilowski scale) in a sample of 64 alcohol dependent patients, at baseline and after a detoxification treatment. Moreover, we recruited 47 non-consanguineous relatives who did not suffer alcohol related disorders and underwent the same tests. In both groups we genotyped 11 genetic variations (rs1800587; rs3087258; rs1799724; 5-HTTLPR; rs1386493; rs1386494; rs1487275; rs1843809; rs4570625; rs2129575; rs6313) located in genes whose impact on alcohol related behaviors and disorders has been hypothesized (IL1A, IL1B, TNF, 5-HTTLPR, TPH2 and HTR2A). We analyzed the epistasis of these genetic variations upon the biological and psychological dimensions in the cases and their relatives. Further on, we analyzed the effects of the combined genetic variations on the short - term detoxification treatment efficacy. Finally, being the only not yet investigated variation within this sample, we analyzed the impact of the rs6313 alone on baseline assessment and treatment efficacy. We detected the following results: the couple rs6313 + rs2129575 affected the Leyton -Trait at admission (p = 0.01) (obsessive-compulsive trait), whilst rs1800587 + 5-HTTLPR impacted the Pilowski test at admission (p = 0.01) (hypochondriac symptoms). These results did not survive Bonferroni correction (p ≤ 0.004). This lack of association may depend on the incomplete gene coverage or on the small sample size which limited the power of the study. On the other hand, it may reflect a substantial absence of relevance of the genotype variants toward the alcohol related investigated dimensions. Nonetheless, the marginal significance we detected could witness an informative correlation worth investigating in larger samples.",
keywords = "5HTPR, Alcohol dependence, Association, Gene, HTR2A, IL1A, IL1B, TNF, TPH2",
author = "Antonio Drago and Ioannis Liappas and Carmine Petio and Diego Albani and Gianluigi Forloni and Petros Malitas and Christina Piperi and Antonis Politis and Tzavellas, {Elias O.} and Zisaki, {Katerina K.} and Francesca Prato and Sara Batelli and Letizia Polito and {De Ronchi}, Diana and Thomas Paparrigopoulos and Anastasios Kalofoutis and Alessandro Serretti",
year = "2009",
month = "7",
doi = "10.3390/ijerph6071980",
language = "English",
volume = "6",
pages = "1980--1990",
journal = "International Journal of Environmental Research and Public Health",
issn = "1661-7827",
publisher = "MDPI AG",
number = "7",

}

TY - JOUR

T1 - Epistasis between IL1A, IL1B, TNF, HTR2A, 5-HTTLPR and TPH2 variations does not impact alcohol dependence disorder features

AU - Drago, Antonio

AU - Liappas, Ioannis

AU - Petio, Carmine

AU - Albani, Diego

AU - Forloni, Gianluigi

AU - Malitas, Petros

AU - Piperi, Christina

AU - Politis, Antonis

AU - Tzavellas, Elias O.

AU - Zisaki, Katerina K.

AU - Prato, Francesca

AU - Batelli, Sara

AU - Polito, Letizia

AU - De Ronchi, Diana

AU - Paparrigopoulos, Thomas

AU - Kalofoutis, Anastasios

AU - Serretti, Alessandro

PY - 2009/7

Y1 - 2009/7

N2 - We assessed a set of biological (HDL, LDL, SGOT,SGPT, GGT, HTc, Hb and T levels) and psychometric variables (investigated through HAM-D, HAM-A, GAS, Liebowitz Social Anxiety Scale, Mark & Mathews Scale, Leyton scale, and Pilowski scale) in a sample of 64 alcohol dependent patients, at baseline and after a detoxification treatment. Moreover, we recruited 47 non-consanguineous relatives who did not suffer alcohol related disorders and underwent the same tests. In both groups we genotyped 11 genetic variations (rs1800587; rs3087258; rs1799724; 5-HTTLPR; rs1386493; rs1386494; rs1487275; rs1843809; rs4570625; rs2129575; rs6313) located in genes whose impact on alcohol related behaviors and disorders has been hypothesized (IL1A, IL1B, TNF, 5-HTTLPR, TPH2 and HTR2A). We analyzed the epistasis of these genetic variations upon the biological and psychological dimensions in the cases and their relatives. Further on, we analyzed the effects of the combined genetic variations on the short - term detoxification treatment efficacy. Finally, being the only not yet investigated variation within this sample, we analyzed the impact of the rs6313 alone on baseline assessment and treatment efficacy. We detected the following results: the couple rs6313 + rs2129575 affected the Leyton -Trait at admission (p = 0.01) (obsessive-compulsive trait), whilst rs1800587 + 5-HTTLPR impacted the Pilowski test at admission (p = 0.01) (hypochondriac symptoms). These results did not survive Bonferroni correction (p ≤ 0.004). This lack of association may depend on the incomplete gene coverage or on the small sample size which limited the power of the study. On the other hand, it may reflect a substantial absence of relevance of the genotype variants toward the alcohol related investigated dimensions. Nonetheless, the marginal significance we detected could witness an informative correlation worth investigating in larger samples.

AB - We assessed a set of biological (HDL, LDL, SGOT,SGPT, GGT, HTc, Hb and T levels) and psychometric variables (investigated through HAM-D, HAM-A, GAS, Liebowitz Social Anxiety Scale, Mark & Mathews Scale, Leyton scale, and Pilowski scale) in a sample of 64 alcohol dependent patients, at baseline and after a detoxification treatment. Moreover, we recruited 47 non-consanguineous relatives who did not suffer alcohol related disorders and underwent the same tests. In both groups we genotyped 11 genetic variations (rs1800587; rs3087258; rs1799724; 5-HTTLPR; rs1386493; rs1386494; rs1487275; rs1843809; rs4570625; rs2129575; rs6313) located in genes whose impact on alcohol related behaviors and disorders has been hypothesized (IL1A, IL1B, TNF, 5-HTTLPR, TPH2 and HTR2A). We analyzed the epistasis of these genetic variations upon the biological and psychological dimensions in the cases and their relatives. Further on, we analyzed the effects of the combined genetic variations on the short - term detoxification treatment efficacy. Finally, being the only not yet investigated variation within this sample, we analyzed the impact of the rs6313 alone on baseline assessment and treatment efficacy. We detected the following results: the couple rs6313 + rs2129575 affected the Leyton -Trait at admission (p = 0.01) (obsessive-compulsive trait), whilst rs1800587 + 5-HTTLPR impacted the Pilowski test at admission (p = 0.01) (hypochondriac symptoms). These results did not survive Bonferroni correction (p ≤ 0.004). This lack of association may depend on the incomplete gene coverage or on the small sample size which limited the power of the study. On the other hand, it may reflect a substantial absence of relevance of the genotype variants toward the alcohol related investigated dimensions. Nonetheless, the marginal significance we detected could witness an informative correlation worth investigating in larger samples.

KW - 5HTPR

KW - Alcohol dependence

KW - Association

KW - Gene

KW - HTR2A

KW - IL1A

KW - IL1B

KW - TNF

KW - TPH2

UR - http://www.scopus.com/inward/record.url?scp=68149087761&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68149087761&partnerID=8YFLogxK

U2 - 10.3390/ijerph6071980

DO - 10.3390/ijerph6071980

M3 - Article

VL - 6

SP - 1980

EP - 1990

JO - International Journal of Environmental Research and Public Health

JF - International Journal of Environmental Research and Public Health

SN - 1661-7827

IS - 7

ER -