TY - JOUR
T1 - Epitope mapping by epitope excision, hydrogen/deuterium exchange, and peptide-panning techniques combined with in silico analysis
AU - Clementi, Nicola
AU - Mancini, Nicasio
AU - Criscuolo, Elena
AU - Cappelletti, Francesca
AU - Clementi, Massimo
AU - Burioni, Roberto
PY - 2014
Y1 - 2014
N2 - The fine characterization of protective B cell epitopes plays a pivotal role in the development of novel vaccines. The development of epitope-based vaccines, in fact, cannot be possible without a clear definition of the antigenic regions involved in the binding between the protective antibody (Ab) and its molecular target. To achieve this result, different epitope-mapping approaches have been widely described (Clementi et al. Drug Discov Today 18(9-10):464-471, 2013). Nowadays, the best way to characterize an Ab bound region is still the resolution of Ab-antigen (Ag) co-crystal structure. Unfortunately, the crystallization approaches are not always feasible. However, different experimental strategies aimed to predict Ab-Ag interaction and followed by in silico analysis of the results may be good surrogate approaches to achieve this result. Here, we review few experimental techniques followed by the use of "basic" informatics tools for the analysis of the results.
AB - The fine characterization of protective B cell epitopes plays a pivotal role in the development of novel vaccines. The development of epitope-based vaccines, in fact, cannot be possible without a clear definition of the antigenic regions involved in the binding between the protective antibody (Ab) and its molecular target. To achieve this result, different epitope-mapping approaches have been widely described (Clementi et al. Drug Discov Today 18(9-10):464-471, 2013). Nowadays, the best way to characterize an Ab bound region is still the resolution of Ab-antigen (Ag) co-crystal structure. Unfortunately, the crystallization approaches are not always feasible. However, different experimental strategies aimed to predict Ab-Ag interaction and followed by in silico analysis of the results may be good surrogate approaches to achieve this result. Here, we review few experimental techniques followed by the use of "basic" informatics tools for the analysis of the results.
KW - B cell epitope
KW - Epitope excision
KW - Epitope-based vaccines
KW - Hydrogen/deuterium exchange
KW - Peptide-panning techniques
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U2 - 10.1007/978-1-62703-992-5_26
DO - 10.1007/978-1-62703-992-5_26
M3 - Article
AN - SCOPUS:84934440711
VL - 1131
SP - 427
EP - 446
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -