Epitope mapping of human chromogranin A by peptide microarrays

Marina Cretich, Renato Longhi, Angelo Corti, Francesco Damin, Gabriele Di Carlo, Valentina Sedini, Marcella Chiari

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter we report on the characterization of linear antigenic sites of human chromogranin A (CgA), a useful tissue and serum marker for neuroendocrine tumours and a precursor of many biologically active peptides. The epitope mapping of CgA has been carried out by peptide microarrays on glass slides coated by a copolymer of N,N-dimethylacrylamide (DMA), N,N-acryloyloxysuccinimide (NAS) and [3-(methacryloyl-oxy) propyl] trimethoxysilyl (MAPS). The microarray support provided sufficient accessibility of the ligand, with no need for a spacer, as the polymer chains prevent interaction of immobilized peptides with substrate. In addition, the polymeric surface constitutes an aqueous micro-environment in which, despite peptide random orientation, linear epitopes are freely exposed. The results reported are in accordance with those obtained in conventional ELISA assays using biotinylated and non-biotinylated peptides.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages221-232
Number of pages12
Volume570
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
Volume570
ISSN (Print)10643745

Keywords

  • Epitope mapping
  • human chromogranin A
  • peptide microarray
  • polymer coating

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Cretich, M., Longhi, R., Corti, A., Damin, F., Carlo, G. D., Sedini, V., & Chiari, M. (2009). Epitope mapping of human chromogranin A by peptide microarrays. In Methods in Molecular Biology (Vol. 570, pp. 221-232). (Methods in Molecular Biology; Vol. 570). https://doi.org/10.1007/978-1-60327-394-7_10