Epitope-specificities of HLA antibodies: The effect of epitope structure on Luminex technique-dependent antibody reactivity

Marianna Resse, Rossella Paolillo, Biagio Pellegrino Minucci, Francesco Cavalca, Amelia Casamassimi, Claudio Napoli

Research output: Contribution to journalArticle

Abstract

The search of HLA antibodies is currently more accessible by solid-phase techniques (Luminex) in the immunized patients leading to an expansion of the antibody patterns. The aim of this study was to investigate low median fluorescence intensity value in unexpected reactivity patterns. Here, we performed HLAMatchmaker analyses to evaluate the potential functional epitopes that can elicit HLA-specific alloantibody responses in a pregnancy-sensitized woman with an epitope defined by the 82LR. Surprisingly, in according to the registry of HLA epitopes, we found that 82LR epitope covered all allelic specificities of our unexpected antibody patterns, shared between Bw4-positive HLA-B antigen and HLA-A23, -A24, -A25 and -A32. This finding is consistent with the verification of HLA ABC epitope recorded in the website-based HLA Epitope Registry and addresses the importance of determining HLA antibody epitope-specificities on Luminex technique-dependent antibody reactivity.

Original languageEnglish
Pages (from-to)297-300
Number of pages4
JournalHuman Immunology
Volume76
Issue number4
DOIs
Publication statusPublished - 2015

Keywords

  • Eplet
  • HLA antibodies
  • HLA Epitope Registry
  • HLAMatchmaker
  • Luminex

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

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