Epitope specificity, antibody-dependent cellular cytotoxicity, and neutralizing activity of antibodies to human immunodeficiency virus type 1 in autoimmune MRL/lpr mice

Valter Lombardi, Roberta Placido, Gabriella Scarlatti, M. Luisa Romiti, Maurizio Mattei, Francesca Mariani, Fabrizio Poccia, Paolo Rossi, Vittorio Colizzi

Research output: Contribution to journalArticlepeer-review

Abstract

The specificity and functional activity of antibodies to human immunodeficiency virus (HIV) in the sera of MRL/lpr mice were analyzed by peptide-ELISA, antibody-dependent cellular cytotoxicity, and virus neutralization. The specificity of the antibodies in the sera of autoimmune MRL/lpr mice was similar to that of HIV-infected persons; the mouse sera specifically recognized the V3 loop of gp120 and immunodominant regions of gp41 and p24. Moreover, such binding was inhibited both by human HIV-positive sera and by soluble peptides in competition experiments. MRL/lpr sera displayed anti-HIV antibody-dependent cellular cytotoxicity using human peripheral blood lymphocytes as effector cells and HIV-infected H9 cells. Furthermore, the fact that MRL/lpr sera neutralized in vitro infectious HIV (both strains IIIB and MN) suggests these antibodies recognize viral epitopes on the membrane of infected T cells.

Original languageEnglish
Pages (from-to)1267-1273
Number of pages7
JournalJournal of Infectious Diseases
Volume167
Issue number6
Publication statusPublished - Jun 1993

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

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