eps15 and eps15R are essential components of the endocytic pathway

Roberta Carbone, Silvia Fré, Gioacchin Iannolo, Francesca Belleudi, Patrizia Mancini, Pier Giuseppe Pelicci, Maria Rosaria Torrisi, Pier Paolo Di Fiore

Research output: Contribution to journalArticle

Abstract

eps15 and eps15R are substrates of the epidermal growth factor (EGF) receptor kinase that are characterized by the presence of a protein:protein interaction domain, the EH domain, and by their ability to bind to the clathrin adaptor protein complex adaptor protein 2. Indirect evidence suggests that eps15 and eps15R are involved in endocytosis. Here we show that microinjection of antibodies against eps15 and eps15R inhibits internalization of EGF and transferrin. In addition, fragments of eps15 (encompassing its EH domains or the COOH-terminal region that binds to adaptor protein 2) inhibit EGF internalization or endocytosis of Sindbis virus. These results demonstrate that eps15 and eps15R are essential components of the endocytic machinery.

Original languageEnglish
Pages (from-to)5498-5504
Number of pages7
JournalCancer Research
Volume57
Issue number24
Publication statusPublished - Dec 15 1997

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Carbone, R., Fré, S., Iannolo, G., Belleudi, F., Mancini, P., Pelicci, P. G., Torrisi, M. R., & Di Fiore, P. P. (1997). eps15 and eps15R are essential components of the endocytic pathway. Cancer Research, 57(24), 5498-5504.