Epstein-Barr virus DNA is present both in CD10 CD77 positive and negative subsets of human tonsillar lymphocytes

Mara Cirone, Ruggero De Maria, Arturo D'Alessandro, Luigi Frati, Alberto Faggioni, Giuseppe Ragona

Research output: Contribution to journalArticlepeer-review

Abstract

We report the fractionation of freshly isolated subsets of tonsillar lymphocytes according to cell density and double sorting for the differential expression of CD10 and CD77, and their analysis for the presence of Epstein Barr virus genome by nested PCR. All the subsets of tonsillar lymphocytes gave unequivocal evidence for the presence of EBV DNA, when obtained from EBV seropositive individuals. Confirmation of all cases examined demonstrates that B lymphocytes from the germinal centers of tonsils are also involved in carrying the EBV infection in vivo.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalCancer Letters
Volume89
Issue number1
DOIs
Publication statusPublished - Feb 10 1995

Keywords

  • CD10 CD77 tonsillar subsets
  • Epstein Barr virus
  • Nested PCR
  • Viral oncology

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Biology

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