Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis

Massimiliano Castellazzi, Carmine Tamborino, Alice Cani, Elena Negri, Eleonora Baldi, Silva Seraceni, Maria Rosaria Tola, Enrico Granieri, Carlo Contini, Enrico Fainardi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cerebrospinal fluid and serum levels and intrathecal synthesis of anti-Epstein-Barr virus (EBV) IgG were measured by enzyme-linked immunosorbent assay in 80 relapsing-remitting multiple sclerosis patients grouped according to clinical and magnetic resonance imaging (MRI) evidence of disease activity. Eighty patients with other inflammatory neurological disorders (OIND) and 80 patients with non-inflammatory neurological disorders (NIND) served as neurological controls. Cerebrospinal fluid concentrations were higher in OIND than in multiple sclerosis (p <0.0001) and NIND (p <0.01) for anti-viral-capsid-antigen (anti-VCA) IgG, in multiple sclerosis than in NIND (p <0.01) and in OIND than in NIND (p <0.05) for anti-EBV nuclear antigen-1 (EBNA-1) IgG. Serum levels were more elevated in OIND than in multiple sclerosis (p <0.05) and in MRI inactive than in MRI active multiple sclerosis (p <0.0001) for anti-VCA IgG, and in multiple sclerosis than in OIND and NIND (p <0.01) for anti-EBNA-1 IgG. Serum titres of anti-VCA and anti-EBNA-1 IgG were also positively (p <0.05) and inversely (p <0.001) correlated, respectively, with the Expanded Disability Status Scale. An intrathecal IgG production of anti-VCA and anti-EBNA-1 IgG, as indicated by Antibody Index, was present only in a limited number of multiple sclerosis patients and controls (range from 1.3 to 6.3%). These findings do not support a direct pathogenetic role of EBV-targeted humoral immune response in multiple sclerosis.

Original languageEnglish
Pages (from-to)883-887
Number of pages5
JournalMultiple Sclerosis Journal
Volume16
Issue number7
DOIs
Publication statusPublished - 2010

Fingerprint

Nervous System Diseases
Human Herpesvirus 4
Multiple Sclerosis
Antibody Formation
Cerebrospinal Fluid
Epstein-Barr Virus Nuclear Antigens
Immunoglobulin G
Serum
Viral Antigens
Capsid
Magnetic Resonance Imaging
Relapsing-Remitting Multiple Sclerosis
Humoral Immunity
Enzyme-Linked Immunosorbent Assay
Antibodies

Keywords

  • cerebrospinal fluid
  • Epstein-Barr virus
  • intrathecal synthesis
  • multiple sclerosis

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology
  • Medicine(all)

Cite this

Castellazzi, M., Tamborino, C., Cani, A., Negri, E., Baldi, E., Seraceni, S., ... Fainardi, E. (2010). Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis. Multiple Sclerosis Journal, 16(7), 883-887. https://doi.org/10.1177/1352458510368051

Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis. / Castellazzi, Massimiliano; Tamborino, Carmine; Cani, Alice; Negri, Elena; Baldi, Eleonora; Seraceni, Silva; Tola, Maria Rosaria; Granieri, Enrico; Contini, Carlo; Fainardi, Enrico.

In: Multiple Sclerosis Journal, Vol. 16, No. 7, 2010, p. 883-887.

Research output: Contribution to journalArticle

Castellazzi, M, Tamborino, C, Cani, A, Negri, E, Baldi, E, Seraceni, S, Tola, MR, Granieri, E, Contini, C & Fainardi, E 2010, 'Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis', Multiple Sclerosis Journal, vol. 16, no. 7, pp. 883-887. https://doi.org/10.1177/1352458510368051
Castellazzi, Massimiliano ; Tamborino, Carmine ; Cani, Alice ; Negri, Elena ; Baldi, Eleonora ; Seraceni, Silva ; Tola, Maria Rosaria ; Granieri, Enrico ; Contini, Carlo ; Fainardi, Enrico. / Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis. In: Multiple Sclerosis Journal. 2010 ; Vol. 16, No. 7. pp. 883-887.
@article{5380f7c7fcfe408ea2117e722ea4fa4c,
title = "Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis",
abstract = "Cerebrospinal fluid and serum levels and intrathecal synthesis of anti-Epstein-Barr virus (EBV) IgG were measured by enzyme-linked immunosorbent assay in 80 relapsing-remitting multiple sclerosis patients grouped according to clinical and magnetic resonance imaging (MRI) evidence of disease activity. Eighty patients with other inflammatory neurological disorders (OIND) and 80 patients with non-inflammatory neurological disorders (NIND) served as neurological controls. Cerebrospinal fluid concentrations were higher in OIND than in multiple sclerosis (p <0.0001) and NIND (p <0.01) for anti-viral-capsid-antigen (anti-VCA) IgG, in multiple sclerosis than in NIND (p <0.01) and in OIND than in NIND (p <0.05) for anti-EBV nuclear antigen-1 (EBNA-1) IgG. Serum levels were more elevated in OIND than in multiple sclerosis (p <0.05) and in MRI inactive than in MRI active multiple sclerosis (p <0.0001) for anti-VCA IgG, and in multiple sclerosis than in OIND and NIND (p <0.01) for anti-EBNA-1 IgG. Serum titres of anti-VCA and anti-EBNA-1 IgG were also positively (p <0.05) and inversely (p <0.001) correlated, respectively, with the Expanded Disability Status Scale. An intrathecal IgG production of anti-VCA and anti-EBNA-1 IgG, as indicated by Antibody Index, was present only in a limited number of multiple sclerosis patients and controls (range from 1.3 to 6.3{\%}). These findings do not support a direct pathogenetic role of EBV-targeted humoral immune response in multiple sclerosis.",
keywords = "cerebrospinal fluid, Epstein-Barr virus, intrathecal synthesis, multiple sclerosis",
author = "Massimiliano Castellazzi and Carmine Tamborino and Alice Cani and Elena Negri and Eleonora Baldi and Silva Seraceni and Tola, {Maria Rosaria} and Enrico Granieri and Carlo Contini and Enrico Fainardi",
year = "2010",
doi = "10.1177/1352458510368051",
language = "English",
volume = "16",
pages = "883--887",
journal = "Multiple Sclerosis",
issn = "1352-4585",
publisher = "SAGE Publications Ltd",
number = "7",

}

TY - JOUR

T1 - Epstein-Barr virus-specific antibody response in cerebrospinal fluid and serum of patients with multiple sclerosis

AU - Castellazzi, Massimiliano

AU - Tamborino, Carmine

AU - Cani, Alice

AU - Negri, Elena

AU - Baldi, Eleonora

AU - Seraceni, Silva

AU - Tola, Maria Rosaria

AU - Granieri, Enrico

AU - Contini, Carlo

AU - Fainardi, Enrico

PY - 2010

Y1 - 2010

N2 - Cerebrospinal fluid and serum levels and intrathecal synthesis of anti-Epstein-Barr virus (EBV) IgG were measured by enzyme-linked immunosorbent assay in 80 relapsing-remitting multiple sclerosis patients grouped according to clinical and magnetic resonance imaging (MRI) evidence of disease activity. Eighty patients with other inflammatory neurological disorders (OIND) and 80 patients with non-inflammatory neurological disorders (NIND) served as neurological controls. Cerebrospinal fluid concentrations were higher in OIND than in multiple sclerosis (p <0.0001) and NIND (p <0.01) for anti-viral-capsid-antigen (anti-VCA) IgG, in multiple sclerosis than in NIND (p <0.01) and in OIND than in NIND (p <0.05) for anti-EBV nuclear antigen-1 (EBNA-1) IgG. Serum levels were more elevated in OIND than in multiple sclerosis (p <0.05) and in MRI inactive than in MRI active multiple sclerosis (p <0.0001) for anti-VCA IgG, and in multiple sclerosis than in OIND and NIND (p <0.01) for anti-EBNA-1 IgG. Serum titres of anti-VCA and anti-EBNA-1 IgG were also positively (p <0.05) and inversely (p <0.001) correlated, respectively, with the Expanded Disability Status Scale. An intrathecal IgG production of anti-VCA and anti-EBNA-1 IgG, as indicated by Antibody Index, was present only in a limited number of multiple sclerosis patients and controls (range from 1.3 to 6.3%). These findings do not support a direct pathogenetic role of EBV-targeted humoral immune response in multiple sclerosis.

AB - Cerebrospinal fluid and serum levels and intrathecal synthesis of anti-Epstein-Barr virus (EBV) IgG were measured by enzyme-linked immunosorbent assay in 80 relapsing-remitting multiple sclerosis patients grouped according to clinical and magnetic resonance imaging (MRI) evidence of disease activity. Eighty patients with other inflammatory neurological disorders (OIND) and 80 patients with non-inflammatory neurological disorders (NIND) served as neurological controls. Cerebrospinal fluid concentrations were higher in OIND than in multiple sclerosis (p <0.0001) and NIND (p <0.01) for anti-viral-capsid-antigen (anti-VCA) IgG, in multiple sclerosis than in NIND (p <0.01) and in OIND than in NIND (p <0.05) for anti-EBV nuclear antigen-1 (EBNA-1) IgG. Serum levels were more elevated in OIND than in multiple sclerosis (p <0.05) and in MRI inactive than in MRI active multiple sclerosis (p <0.0001) for anti-VCA IgG, and in multiple sclerosis than in OIND and NIND (p <0.01) for anti-EBNA-1 IgG. Serum titres of anti-VCA and anti-EBNA-1 IgG were also positively (p <0.05) and inversely (p <0.001) correlated, respectively, with the Expanded Disability Status Scale. An intrathecal IgG production of anti-VCA and anti-EBNA-1 IgG, as indicated by Antibody Index, was present only in a limited number of multiple sclerosis patients and controls (range from 1.3 to 6.3%). These findings do not support a direct pathogenetic role of EBV-targeted humoral immune response in multiple sclerosis.

KW - cerebrospinal fluid

KW - Epstein-Barr virus

KW - intrathecal synthesis

KW - multiple sclerosis

UR - http://www.scopus.com/inward/record.url?scp=77955020345&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955020345&partnerID=8YFLogxK

U2 - 10.1177/1352458510368051

DO - 10.1177/1352458510368051

M3 - Article

VL - 16

SP - 883

EP - 887

JO - Multiple Sclerosis

JF - Multiple Sclerosis

SN - 1352-4585

IS - 7

ER -