erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells

P. P. Di Fiore, J. H. Pierce, M. H. Kraus, O. Segatto, C. R. King, S. A. Aaronson

Research output: Contribution to journalArticle

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Abstract

A wide variety of human tumors contain an amplified or overexpressed erbB-2 gene, which encodes a growth factor receptor-like protein. When erbB-2 complementary DNA was expressed in NIH/3T3 cells under the control of the SV40 promoter, the gene lacked transforming activity despite expression of detectable levels of the erbB-2 protein. A further five- to tenfold increase in its expression under influence of the long terminal repeat of Moloney murine leukemia virus was associated with activation of erbB-2 as a potent oncogene. The high levels of the erbB-2 product associated with malignant transformation of NIH/3T3 cells were observed in human mammary tumor cells that overexpressed this gene. These findings demonstrate a new mechanism for acquisition of oncogenic properties by genes encoding growth factor receptor-like proteins and provide a functional basis for the role of their overexpression in the development of human malignancies.

Original languageEnglish
Pages (from-to)178-182
Number of pages5
JournalScience
Volume237
Issue number4811
DOIs
Publication statusPublished - 1987

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NIH 3T3 Cells
Oncogenes
Growth Factor Receptors
erbB-2 Genes
Moloney murine leukemia virus
Proteins
Terminal Repeat Sequences
Human Development
Genes
Neoplasms
Complementary DNA
Breast Neoplasms

ASJC Scopus subject areas

  • General

Cite this

Di Fiore, P. P., Pierce, J. H., Kraus, M. H., Segatto, O., King, C. R., & Aaronson, S. A. (1987). erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells. Science, 237(4811), 178-182. https://doi.org/10.1126/science.2885917

erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells. / Di Fiore, P. P.; Pierce, J. H.; Kraus, M. H.; Segatto, O.; King, C. R.; Aaronson, S. A.

In: Science, Vol. 237, No. 4811, 1987, p. 178-182.

Research output: Contribution to journalArticle

Di Fiore, PP, Pierce, JH, Kraus, MH, Segatto, O, King, CR & Aaronson, SA 1987, 'erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells', Science, vol. 237, no. 4811, pp. 178-182. https://doi.org/10.1126/science.2885917
Di Fiore, P. P. ; Pierce, J. H. ; Kraus, M. H. ; Segatto, O. ; King, C. R. ; Aaronson, S. A. / erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells. In: Science. 1987 ; Vol. 237, No. 4811. pp. 178-182.
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