erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells

P. P. Di Fiore, J. H. Pierce, M. H. Kraus, O. Segatto, C. R. King, S. A. Aaronson

Research output: Contribution to journalArticlepeer-review

Abstract

A wide variety of human tumors contain an amplified or overexpressed erbB-2 gene, which encodes a growth factor receptor-like protein. When erbB-2 complementary DNA was expressed in NIH/3T3 cells under the control of the SV40 promoter, the gene lacked transforming activity despite expression of detectable levels of the erbB-2 protein. A further five- to tenfold increase in its expression under influence of the long terminal repeat of Moloney murine leukemia virus was associated with activation of erbB-2 as a potent oncogene. The high levels of the erbB-2 product associated with malignant transformation of NIH/3T3 cells were observed in human mammary tumor cells that overexpressed this gene. These findings demonstrate a new mechanism for acquisition of oncogenic properties by genes encoding growth factor receptor-like proteins and provide a functional basis for the role of their overexpression in the development of human malignancies.

Original languageEnglish
Pages (from-to)178-182
Number of pages5
JournalScience
Volume237
Issue number4811
DOIs
Publication statusPublished - 1987

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells'. Together they form a unique fingerprint.

Cite this