Erythrocyte ferritin concentration: Analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay

Cristina Novembrino, Antonella Porcella, Dario Conte, Amedeo F. de Vecchi, Gherardo Buccianti, Silvia Lonati, Lorena Duca, Alberto Ciani, Fabrizia Bamonti-Catena

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Together with serum ferritin, erythrocyte ferritin can be a valuable diagnostic tool for evaluating the degree of impaired iron metabolism in different diseases. We collected peripheral blood samples from 64 subjects (22 healthy volunteers, 20 patients with hereditary hemochromatosis, and 22 patients on regular hemodialysis with secondary anemia) to evaluate whether an immunoenzymatic method generally used for serum ferritin can also be used to determine erythrocyte ferritin levels under various conditions of body iron status. Serum and erythrocyte ferritin levels were assayed in parallel using a microparticle enzyme immunoassay (MEIA) IMx-Ferritin kit and an IMx analyzer. The inter-assay imprecision of the serum and erythrocyte ferritin assays was 4.9% and 5.05%, the intra-assay imprecision was 2.2% and 2.3%, and the mean recovery was 102% (range 96-105%) and 101% (range 99-105%), respectively. Both serum and erythrocyte ferritin assays showed a detection limit of 1 μg/L and good linearity (R2=0.99) in the intervals 13.9-443 and 3.9-135.6 μg/L, respectively. Our findings demonstrate that the IMx-Ferritin assay currently used to measure serum ferritin levels can also be adopted to measure erythrocyte ferritin insofar as it clearly discriminates high and low erythrocyte ferritin levels in cases of both iron overload and deficiency.

Original languageEnglish
Pages (from-to)449-453
Number of pages5
JournalClinical Chemistry and Laboratory Medicine
Volume43
Issue number4
DOIs
Publication statusPublished - Feb 7 2005

Fingerprint

Ferritins
Assays
Erythrocytes
Serum
Iron
Iron Overload
Hemochromatosis
Immunoenzyme Techniques
Metabolism
Renal Dialysis
Limit of Detection
Anemia
Healthy Volunteers
Blood
Recovery

Keywords

  • Analytical performance
  • Erythrocyte ferritin
  • Iron disorders
  • Microparticle enzyme immunoassay (MEIA)
  • Serum ferritin

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Erythrocyte ferritin concentration : Analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay. / Novembrino, Cristina; Porcella, Antonella; Conte, Dario; de Vecchi, Amedeo F.; Buccianti, Gherardo; Lonati, Silvia; Duca, Lorena; Ciani, Alberto; Bamonti-Catena, Fabrizia.

In: Clinical Chemistry and Laboratory Medicine, Vol. 43, No. 4, 07.02.2005, p. 449-453.

Research output: Contribution to journalArticle

Novembrino, Cristina ; Porcella, Antonella ; Conte, Dario ; de Vecchi, Amedeo F. ; Buccianti, Gherardo ; Lonati, Silvia ; Duca, Lorena ; Ciani, Alberto ; Bamonti-Catena, Fabrizia. / Erythrocyte ferritin concentration : Analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay. In: Clinical Chemistry and Laboratory Medicine. 2005 ; Vol. 43, No. 4. pp. 449-453.
@article{d46513c1c17b498c88de025bce4638ed,
title = "Erythrocyte ferritin concentration: Analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay",
abstract = "Together with serum ferritin, erythrocyte ferritin can be a valuable diagnostic tool for evaluating the degree of impaired iron metabolism in different diseases. We collected peripheral blood samples from 64 subjects (22 healthy volunteers, 20 patients with hereditary hemochromatosis, and 22 patients on regular hemodialysis with secondary anemia) to evaluate whether an immunoenzymatic method generally used for serum ferritin can also be used to determine erythrocyte ferritin levels under various conditions of body iron status. Serum and erythrocyte ferritin levels were assayed in parallel using a microparticle enzyme immunoassay (MEIA) IMx-Ferritin kit and an IMx analyzer. The inter-assay imprecision of the serum and erythrocyte ferritin assays was 4.9{\%} and 5.05{\%}, the intra-assay imprecision was 2.2{\%} and 2.3{\%}, and the mean recovery was 102{\%} (range 96-105{\%}) and 101{\%} (range 99-105{\%}), respectively. Both serum and erythrocyte ferritin assays showed a detection limit of 1 μg/L and good linearity (R2=0.99) in the intervals 13.9-443 and 3.9-135.6 μg/L, respectively. Our findings demonstrate that the IMx-Ferritin assay currently used to measure serum ferritin levels can also be adopted to measure erythrocyte ferritin insofar as it clearly discriminates high and low erythrocyte ferritin levels in cases of both iron overload and deficiency.",
keywords = "Analytical performance, Erythrocyte ferritin, Iron disorders, Microparticle enzyme immunoassay (MEIA), Serum ferritin",
author = "Cristina Novembrino and Antonella Porcella and Dario Conte and {de Vecchi}, {Amedeo F.} and Gherardo Buccianti and Silvia Lonati and Lorena Duca and Alberto Ciani and Fabrizia Bamonti-Catena",
year = "2005",
month = "2",
day = "7",
doi = "10.1515/CCLM.2005.079",
language = "English",
volume = "43",
pages = "449--453",
journal = "Clinical Chemistry and Laboratory Medicine",
issn = "1434-6621",
publisher = "Walter de Gruyter GmbH",
number = "4",

}

TY - JOUR

T1 - Erythrocyte ferritin concentration

T2 - Analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay

AU - Novembrino, Cristina

AU - Porcella, Antonella

AU - Conte, Dario

AU - de Vecchi, Amedeo F.

AU - Buccianti, Gherardo

AU - Lonati, Silvia

AU - Duca, Lorena

AU - Ciani, Alberto

AU - Bamonti-Catena, Fabrizia

PY - 2005/2/7

Y1 - 2005/2/7

N2 - Together with serum ferritin, erythrocyte ferritin can be a valuable diagnostic tool for evaluating the degree of impaired iron metabolism in different diseases. We collected peripheral blood samples from 64 subjects (22 healthy volunteers, 20 patients with hereditary hemochromatosis, and 22 patients on regular hemodialysis with secondary anemia) to evaluate whether an immunoenzymatic method generally used for serum ferritin can also be used to determine erythrocyte ferritin levels under various conditions of body iron status. Serum and erythrocyte ferritin levels were assayed in parallel using a microparticle enzyme immunoassay (MEIA) IMx-Ferritin kit and an IMx analyzer. The inter-assay imprecision of the serum and erythrocyte ferritin assays was 4.9% and 5.05%, the intra-assay imprecision was 2.2% and 2.3%, and the mean recovery was 102% (range 96-105%) and 101% (range 99-105%), respectively. Both serum and erythrocyte ferritin assays showed a detection limit of 1 μg/L and good linearity (R2=0.99) in the intervals 13.9-443 and 3.9-135.6 μg/L, respectively. Our findings demonstrate that the IMx-Ferritin assay currently used to measure serum ferritin levels can also be adopted to measure erythrocyte ferritin insofar as it clearly discriminates high and low erythrocyte ferritin levels in cases of both iron overload and deficiency.

AB - Together with serum ferritin, erythrocyte ferritin can be a valuable diagnostic tool for evaluating the degree of impaired iron metabolism in different diseases. We collected peripheral blood samples from 64 subjects (22 healthy volunteers, 20 patients with hereditary hemochromatosis, and 22 patients on regular hemodialysis with secondary anemia) to evaluate whether an immunoenzymatic method generally used for serum ferritin can also be used to determine erythrocyte ferritin levels under various conditions of body iron status. Serum and erythrocyte ferritin levels were assayed in parallel using a microparticle enzyme immunoassay (MEIA) IMx-Ferritin kit and an IMx analyzer. The inter-assay imprecision of the serum and erythrocyte ferritin assays was 4.9% and 5.05%, the intra-assay imprecision was 2.2% and 2.3%, and the mean recovery was 102% (range 96-105%) and 101% (range 99-105%), respectively. Both serum and erythrocyte ferritin assays showed a detection limit of 1 μg/L and good linearity (R2=0.99) in the intervals 13.9-443 and 3.9-135.6 μg/L, respectively. Our findings demonstrate that the IMx-Ferritin assay currently used to measure serum ferritin levels can also be adopted to measure erythrocyte ferritin insofar as it clearly discriminates high and low erythrocyte ferritin levels in cases of both iron overload and deficiency.

KW - Analytical performance

KW - Erythrocyte ferritin

KW - Iron disorders

KW - Microparticle enzyme immunoassay (MEIA)

KW - Serum ferritin

UR - http://www.scopus.com/inward/record.url?scp=24044441318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24044441318&partnerID=8YFLogxK

U2 - 10.1515/CCLM.2005.079

DO - 10.1515/CCLM.2005.079

M3 - Article

C2 - 15899663

AN - SCOPUS:24044441318

VL - 43

SP - 449

EP - 453

JO - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

SN - 1434-6621

IS - 4

ER -