Erythrocytes as carriers of antisense PNA addressed against HIV-1 gag-pol transframe domain

Alessandra Fraternale, Maria Filomena Paoletti, Anna Casabianca, Chiara Orlandi, Enrico Millo, Emanuela Balestra, Gianluca Damonte, Carlo Federico Perno, Mauro Magnani

Research output: Contribution to journalArticlepeer-review

Abstract

PNAPR2 is a peptide nucleic acid (PNA) complementary to a sequence of the viral protease-encoding gene, effective in blocking HIV release, when used at high doses. Erythrocytes (RBC) were used to target PNA PR2 to the macrophage compartment. The antiviral activity was assessed in human HIV-infected macrophages both as inhibition of p24 production and reduction of HIV DNA content. PNAPR2, either added to the medium at a concentration of 100 μM or loaded into RBC at about 40 μM, inhibited p24 production 80 compared with infected samples and reduced HIV DNA content by 83 and 90, respectively. The results show that (1) a stronger anti-HIV effect is achievable with higher doses of PNAPR2, both when given free and encapsulated into RBC; (2) the antiviral effect obtained by free PNA PR2 at a concentration of 100 μM is achievable by encapsulating it into RBC at a concentration of 40 μM, suggesting that RBC can be used as a delivery system to increase the antisense effect of PNAPR2.

Original languageEnglish
Pages (from-to)278-285
Number of pages8
JournalJournal of Drug Targeting
Volume17
Issue number4
DOIs
Publication statusPublished - May 2009

Keywords

  • Delivery
  • Erythrocytes (RBC)
  • HIV
  • Macrophages
  • Peptide nucleic acid (PNA)

ASJC Scopus subject areas

  • Pharmaceutical Science

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