Abstract
Colony formation by erythroid precursor derived from umbilical cord blood was studied in a methylcellulose culture system. Mononuclear cells were obtained by centrifugation in Ficoll-Hypaque and incubated up to 15 days in 1 ml of methylcellulose culture containing from 0-4 I.U./ml of erythropoietin (EPO) and 3.105 cells/ml. No large erythroid colonies formed in the absence of EPO. Small colonies begin to appear at day 7 and at day 12-13 they reach maximum growth. Colonies are composed of either mature or immature cells suggesting that this cell source contains heterogeneous populations of erythroid colony progenitors.
| Original language | English |
|---|---|
| Pages (from-to) | 95-98 |
| Number of pages | 4 |
| Journal | International Journal of Biological Research in Pregnancy |
| Volume | 1 |
| Issue number | 3 |
| Publication status | Published - 1980 |
ASJC Scopus subject areas
- Medicine(all)