Colony formation by erythroid precursor derived from umbilical cord blood was studied in a methylcellulose culture system. Mononuclear cells were obtained by centrifugation in Ficoll-Hypaque and incubated up to 15 days in 1 ml of methylcellulose culture containing from 0-4 I.U./ml of erythropoietin (EPO) and 3.105 cells/ml. No large erythroid colonies formed in the absence of EPO. Small colonies begin to appear at day 7 and at day 12-13 they reach maximum growth. Colonies are composed of either mature or immature cells suggesting that this cell source contains heterogeneous populations of erythroid colony progenitors.
|Number of pages||4|
|Journal||International Journal of Biological Research in Pregnancy|
|Publication status||Published - 1980|
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