Erythroid differentiation of Friend erythroleukemic cells: a 'variant' cell line endowed with an early expression of erythroid markers

E. Affabris, S. Pulciani, G. B. Rossi, M. Capobianchi

Research output: Contribution to journalArticle

Abstract

A cloned variant (A°1-Cl1) of murine erythroleukemic cells (FLC) has been isolated from non mutagenized 745A parental cell line, and its phenotype has been analyzed. Cells of this clone as well as 745A cells do not spontaneously differentiate. When cultured in the presence of Dimethyl Sulfoxide (DMSO), the A°1-Cl1 cells exhibit an earlier and more pronounced expression of erythroid markers than do parental cells. Exposure of A°1-Cl1 and 745A cells to a battery of other stimulatory drugs such as hexamethylenebisacetamide (HMBA), hypoxanthyne and butyric acid, also results in a marked shift of the cell populations towards the more mature stages of erythroid differentiation. The 745A cells respond better to butyric acid than A°1-Cl1 cells whereas the contrary is true for HMBA, and no significant differences have been observed with the other two 'inducers'.

Original languageEnglish
Pages (from-to)15-25
Number of pages11
JournalMicrobiologica
VolumeVOL.1
Publication statusPublished - 1978

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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