Erythrophagocytosis increases the expression of erythroid potentiating activity mRNA in human monocyte-macrophages

G. Bergamaschi, H. H. Recalde, L. Ponchio, V. Rosti, M. Cazzola

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to evaluate whether the erythropoietic response to hemolysis can be mediated by other regulatory peptides in addition to erythropoietin. For this purpose, we have investigated the influence of erythrophagocytosis by human monocytes and macrophages on the mRNA expression of several growth factor genes, including interleukin-3 (IL- 3), granulocyte/macrophage colony-stimulating factor (GM-CSF) and erythroid potentiating activity (EPA), which are supposed to influence erythropoiesis. Immunologically mediated erythrophagocytosis increased the expression of EPA mRNA (2 to 3 times). Such increase appeared to be specifically associated with phagocytosis of erythrocytes, since phagocytosis of yeast microorganisms or antibody-coated latex particles had no effect on EPA gene expression. Yeast, however, powerfully stimulated the expression of GM-CSF, granulocyte colony-stimulating factor (G-CSF) and interleukin-6 (IL-6) mRNAs which, with the exception of G-CSF, were not influenced by erythrophagocytosis. Erythropoietin and IL-3 mRNAs were never detected in cultured monocytes, either in control or in treated samples. Our findings may suggest that phagocytosis of erythrocytes by monocytes/macrophages increases the expression, and possibly the production, of EPA. This could in turn potentiate the erythropoietic response to extravascular hemolysis by increasing the number of cells responsive to erythropoietin. Thus, EPA might be a mediator of an end-product positive feedback on the rate of red cell production.

Original languageEnglish
Pages (from-to)70-73
Number of pages4
JournalExperimental Hematology
Volume21
Issue number1
Publication statusPublished - 1993

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Monocytes
Erythropoietin
Macrophages
Phagocytosis
Messenger RNA
Interleukin-3
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Hemolysis
Yeasts
Erythrocytes
Erythropoiesis
Microspheres
Interleukin-6
Intercellular Signaling Peptides and Proteins
Cell Count
Gene Expression
Peptides
Antibodies
Genes

Keywords

  • Erythroid potentiating activity
  • Erythropoiesis
  • Monocytes
  • Phagocytosis

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Erythrophagocytosis increases the expression of erythroid potentiating activity mRNA in human monocyte-macrophages. / Bergamaschi, G.; Recalde, H. H.; Ponchio, L.; Rosti, V.; Cazzola, M.

In: Experimental Hematology, Vol. 21, No. 1, 1993, p. 70-73.

Research output: Contribution to journalArticle

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