Erythropoietin does not preserve motor neurons in a mouse model of familiai ALS

Giuliano Grignaschi, Eleonora Zennaro, Massimo Tortarolo, Novella Calvaresi, Caterina Bendotti

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Recombinant human EPO (r-Hu-EPO) protects cultured motor neurons from kainate- and serum deprivation-induced toxicity and readily enters the CNS after systemic injection. We examined the effect of rHuEPO in transgenic mice expressing the human Cu/Zn dependent-superoxide dismutase with G93A mutation (SOD1G93A), a model of familial amyotrophic lateral sclerosis. rHuEPO (4 unit/g BW s.c. three times/week), increased the haematocrit and induced a slight delay in impairment of motor function as measured by the rotating bar test. However, it did not prolong life span or reduce motor neuron loss in lumbar spinal cord. The effect on motor function may be due to the improvement of skeletal muscle oxygenation induced by chronic EPO administration.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalAmyotrophic Lateral Sclerosis
Volume8
Issue number1
DOIs
Publication statusPublished - 2007

Fingerprint

Motor Neurons
Erythropoietin
Spinal Cord
Kainic Acid
Hematocrit
Transgenic Mice
Skeletal Muscle
Mutation
Injections
Serum
Amyotrophic lateral sclerosis 1
Superoxide Dismutase-1

Keywords

  • Amyotrophic lateral sclerosis
  • Erythropoietin
  • Motor neuron
  • Transgenic SOD1G93A mice

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Erythropoietin does not preserve motor neurons in a mouse model of familiai ALS. / Grignaschi, Giuliano; Zennaro, Eleonora; Tortarolo, Massimo; Calvaresi, Novella; Bendotti, Caterina.

In: Amyotrophic Lateral Sclerosis, Vol. 8, No. 1, 2007, p. 31-35.

Research output: Contribution to journalArticle

Grignaschi, Giuliano ; Zennaro, Eleonora ; Tortarolo, Massimo ; Calvaresi, Novella ; Bendotti, Caterina. / Erythropoietin does not preserve motor neurons in a mouse model of familiai ALS. In: Amyotrophic Lateral Sclerosis. 2007 ; Vol. 8, No. 1. pp. 31-35.
@article{c0b10fe2cb96407488b66630478ce41c,
title = "Erythropoietin does not preserve motor neurons in a mouse model of familiai ALS",
abstract = "Recombinant human EPO (r-Hu-EPO) protects cultured motor neurons from kainate- and serum deprivation-induced toxicity and readily enters the CNS after systemic injection. We examined the effect of rHuEPO in transgenic mice expressing the human Cu/Zn dependent-superoxide dismutase with G93A mutation (SOD1G93A), a model of familial amyotrophic lateral sclerosis. rHuEPO (4 unit/g BW s.c. three times/week), increased the haematocrit and induced a slight delay in impairment of motor function as measured by the rotating bar test. However, it did not prolong life span or reduce motor neuron loss in lumbar spinal cord. The effect on motor function may be due to the improvement of skeletal muscle oxygenation induced by chronic EPO administration.",
keywords = "Amyotrophic lateral sclerosis, Erythropoietin, Motor neuron, Transgenic SOD1G93A mice",
author = "Giuliano Grignaschi and Eleonora Zennaro and Massimo Tortarolo and Novella Calvaresi and Caterina Bendotti",
year = "2007",
doi = "10.1080/17482960600783456",
language = "English",
volume = "8",
pages = "31--35",
journal = "Amyotrophic Lateral Sclerosis",
issn = "1748-2968",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Erythropoietin does not preserve motor neurons in a mouse model of familiai ALS

AU - Grignaschi, Giuliano

AU - Zennaro, Eleonora

AU - Tortarolo, Massimo

AU - Calvaresi, Novella

AU - Bendotti, Caterina

PY - 2007

Y1 - 2007

N2 - Recombinant human EPO (r-Hu-EPO) protects cultured motor neurons from kainate- and serum deprivation-induced toxicity and readily enters the CNS after systemic injection. We examined the effect of rHuEPO in transgenic mice expressing the human Cu/Zn dependent-superoxide dismutase with G93A mutation (SOD1G93A), a model of familial amyotrophic lateral sclerosis. rHuEPO (4 unit/g BW s.c. three times/week), increased the haematocrit and induced a slight delay in impairment of motor function as measured by the rotating bar test. However, it did not prolong life span or reduce motor neuron loss in lumbar spinal cord. The effect on motor function may be due to the improvement of skeletal muscle oxygenation induced by chronic EPO administration.

AB - Recombinant human EPO (r-Hu-EPO) protects cultured motor neurons from kainate- and serum deprivation-induced toxicity and readily enters the CNS after systemic injection. We examined the effect of rHuEPO in transgenic mice expressing the human Cu/Zn dependent-superoxide dismutase with G93A mutation (SOD1G93A), a model of familial amyotrophic lateral sclerosis. rHuEPO (4 unit/g BW s.c. three times/week), increased the haematocrit and induced a slight delay in impairment of motor function as measured by the rotating bar test. However, it did not prolong life span or reduce motor neuron loss in lumbar spinal cord. The effect on motor function may be due to the improvement of skeletal muscle oxygenation induced by chronic EPO administration.

KW - Amyotrophic lateral sclerosis

KW - Erythropoietin

KW - Motor neuron

KW - Transgenic SOD1G93A mice

UR - http://www.scopus.com/inward/record.url?scp=33847312667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847312667&partnerID=8YFLogxK

U2 - 10.1080/17482960600783456

DO - 10.1080/17482960600783456

M3 - Article

VL - 8

SP - 31

EP - 35

JO - Amyotrophic Lateral Sclerosis

JF - Amyotrophic Lateral Sclerosis

SN - 1748-2968

IS - 1

ER -