Erythropoietin expression in primary rat Sertoli and peritubular myoid cells

Massimo Magnanti, Orietta Gandini, Laura Giuliani, Paola Gazzaniga, Hugo H. Marti, Angela Gradilone, Luigi Frati, Anna Maria Aglianò, Max Gassmann

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Kidney and liver are the major organs of erythropoietin (Epo) synthesis. However, Epo messenger RNA (mRNA) has been detected in several organs, such as brain, lung, and testis. Furthermore, functional Epo receptors have been demonstrated on different cell types, including rat Leydig cells. The aim of the study was to identify testicular cells expressing Epo mRNA and to quantitate its levels by competitive reverse transcriptase-polymerase chain reaction (RTPCR). Besides whole testis, Epo transcripts were found in Sertoli and peritubular myoid cells, while no signal was detected in Leydig cells. Exposure of Sertoli cells to CoCl2 led to an increase of Epo mRNA level. Semiquantitative competitive RT-PCR presented an increase in the level of Epo mRNA in Sertoli cells stimulated by follicle-stimulating hormone, while exposure of peritubular myoid cells cultures to testosterone reduced Epo mRNA expression. Due to the blood-testis barrier, basal expression of Epo suggests a not yet defined function of this hormone in testis.

Original languageEnglish
Pages (from-to)2872-2874
Number of pages3
JournalBlood
Volume98
Issue number9
DOIs
Publication statusPublished - Nov 1 2001

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Erythropoietin
Rats
Messenger RNA
Testis
Leydig Cells
Sertoli Cells
Blood-Testis Barrier
Erythropoietin Receptors
Polymerase chain reaction
RNA-Directed DNA Polymerase
Follicle Stimulating Hormone
Reverse Transcriptase Polymerase Chain Reaction
Cell culture
Liver
Testosterone
Brain
Blood
Cell Culture Techniques
Cells
Hormones

ASJC Scopus subject areas

  • Hematology

Cite this

Magnanti, M., Gandini, O., Giuliani, L., Gazzaniga, P., Marti, H. H., Gradilone, A., ... Gassmann, M. (2001). Erythropoietin expression in primary rat Sertoli and peritubular myoid cells. Blood, 98(9), 2872-2874. https://doi.org/10.1182/blood.V98.9.2872

Erythropoietin expression in primary rat Sertoli and peritubular myoid cells. / Magnanti, Massimo; Gandini, Orietta; Giuliani, Laura; Gazzaniga, Paola; Marti, Hugo H.; Gradilone, Angela; Frati, Luigi; Aglianò, Anna Maria; Gassmann, Max.

In: Blood, Vol. 98, No. 9, 01.11.2001, p. 2872-2874.

Research output: Contribution to journalArticle

Magnanti, M, Gandini, O, Giuliani, L, Gazzaniga, P, Marti, HH, Gradilone, A, Frati, L, Aglianò, AM & Gassmann, M 2001, 'Erythropoietin expression in primary rat Sertoli and peritubular myoid cells', Blood, vol. 98, no. 9, pp. 2872-2874. https://doi.org/10.1182/blood.V98.9.2872
Magnanti M, Gandini O, Giuliani L, Gazzaniga P, Marti HH, Gradilone A et al. Erythropoietin expression in primary rat Sertoli and peritubular myoid cells. Blood. 2001 Nov 1;98(9):2872-2874. https://doi.org/10.1182/blood.V98.9.2872
Magnanti, Massimo ; Gandini, Orietta ; Giuliani, Laura ; Gazzaniga, Paola ; Marti, Hugo H. ; Gradilone, Angela ; Frati, Luigi ; Aglianò, Anna Maria ; Gassmann, Max. / Erythropoietin expression in primary rat Sertoli and peritubular myoid cells. In: Blood. 2001 ; Vol. 98, No. 9. pp. 2872-2874.
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