Escherichia coli cytotoxic necrotizing factor 1

Evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase

C. Fiorentini, G. Donelli, P. Matarrese, A. Fabbri, S. Paradisi, P. Boquet

Research output: Contribution to journalArticle

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Abstract

Cytotoxic necrotizing factor type 1 (CNF1) induces in HEp-2 cells an increase in F-actin structures, which was detectable by fluorescence- activated cell sorter analysis 24 h after addition of this factor to the culture medium. Increase in F-actin was correlated with the augmentation of both the cell volume and the total cell actin content. Actin assembly- disassembly is controlled by small GTP-binding proteins of the Rho family, which have been reported recently to be modified by CNF1 treatment. Clostridium difficile toxin B and Clostridium botulinum exoenzyme C3, both known to act on the Rho GTPase, were used as biological tools to study the effect of CNF1 on this protein. CNF1 incubated before, during, or after exposure to the chimeric toxin C3B (which is the product of a genetic fusion between the DNA coding for C3 and the one coding for the B fragment of diphtheria toxin) protected HEp-2 cells from the disruption of F-actin structures caused by inactivation of the Rho GTPase through its ADP- ribosylation. On the other hand, C. difficile toxin B cytopathic effect was not observed upon preincubation of cells with CNF1. Toxins acting through a Rho-independent mechanism, such as cytochalasin D and Clostridium spiroforme iota-like toxin, could not be modified in their cellular activities by CNF1 treatment. All of our results suggest that CNF1 modifies the Rho molecule, thus probably protecting this GTPase from further bacterial toxin modification.

Original languageEnglish
Pages (from-to)3936-3944
Number of pages9
JournalInfection and Immunity
Volume63
Issue number10
Publication statusPublished - 1995

Fingerprint

rho GTP-Binding Proteins
Actins
Escherichia coli
Bacterial Toxins
Immunotoxins
Cytochalasin D
GTP Phosphohydrolases
cytotoxic necrotizing factor type 1
Cell Size
Adenosine Diphosphate
Culture Media
Fluorescence
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Fiorentini, C., Donelli, G., Matarrese, P., Fabbri, A., Paradisi, S., & Boquet, P. (1995). Escherichia coli cytotoxic necrotizing factor 1: Evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase. Infection and Immunity, 63(10), 3936-3944.

Escherichia coli cytotoxic necrotizing factor 1 : Evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase. / Fiorentini, C.; Donelli, G.; Matarrese, P.; Fabbri, A.; Paradisi, S.; Boquet, P.

In: Infection and Immunity, Vol. 63, No. 10, 1995, p. 3936-3944.

Research output: Contribution to journalArticle

Fiorentini, C, Donelli, G, Matarrese, P, Fabbri, A, Paradisi, S & Boquet, P 1995, 'Escherichia coli cytotoxic necrotizing factor 1: Evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase', Infection and Immunity, vol. 63, no. 10, pp. 3936-3944.
Fiorentini, C. ; Donelli, G. ; Matarrese, P. ; Fabbri, A. ; Paradisi, S. ; Boquet, P. / Escherichia coli cytotoxic necrotizing factor 1 : Evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase. In: Infection and Immunity. 1995 ; Vol. 63, No. 10. pp. 3936-3944.
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