Escherichia coli porin induces proinflammatory alterations in renal tubular cells

Luigi Biancone, Pier Giulio Conaldi, Antonio Toniolo, Giovanni Camussi

Research output: Contribution to journalArticle

Abstract

Background/Aims: Several bacterial components may contribute to the development of renal injury during Gram-negative sepsis. In the present study, we evaluated the proinflammatory effect on cultured human proximal tubular epithelial cells (PTEC) of a 36-kD porin purified from Escherichia coli Methods: PTEC were stimulated with E. coli porin to evaluate 45Ca2+ influx, cytoskeleton changes, generation of reactive oxygen species (ROS) as detected by chemiluminescence and cytochrome c reduction. Production of TNF- α, IL-6 and IL-8 was evaluated at the mRNA and protein levels. Results: Stimulation of PTEC with porin was followed by a rapid and sustained 45Ca2+ influx and by an altered distribution of actin fibers and of vinculin streaks. Porin was able to induce generation of ROS and production of proinflammatory cytokines from PTEC. TNF-α production peaked at 6 h after exposure to porin and preceded that of IL-6 and IL-8. Conclusion: E. coli porin - at doses attainable in vivo - appears to stimulate PTEC to produce ROS and cytokines. Porin-induced PTEC activation may contribute to renal injury in the course of Gram-negative infection.

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalExperimental Nephrology
Volume5
Issue number4
Publication statusPublished - Jul 1997

Keywords

  • Endotoxin
  • Porin
  • Septic shock
  • Tubular cell

ASJC Scopus subject areas

  • Nephrology

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