Essential requirement of reduced glutathione (GSH) for the anti-oxidant effect of the flavonoid quercetin

Roberta Ferraresi, Leonarda Troiano, Erika Roat, Enrico Lugli, Elisa Nemes, Milena Nasi, Marcello Pinti, Maria I. Garcia Fernandez, Edwin L. Copper, Andrea Cossarizza

Research output: Contribution to journalArticle

Abstract

We have analyzed the anti- or pro-oxidant effects of the flavonoid quercetin (QU) by evaluating, in U937 cell line, hydrogen peroxide (H2O2), superoxide anion (O2 -), reduced glutathione (GSH) content, mitochondrial membrane potential, DNA content, phosphatidylserine exposure on the outer face of the plasma membrane and cell viability. Polychromatic flow cytometry was used to evaluate in the same cells several functional parameters. For short periods of treatment QU exerted an anti-oxidant effect (decrease in H2O2 levels), whereas for long periods it showed a pro-oxidant activity (increase in O2 -). In these conditions, GSH content was reduced, and this correlated with a lack of anti-oxidant activity of QU, which in turn could be correlated with proapoptotic activity of this molecule. Thus, QU can exert different effects (anti-/prooxidant) depending on exposure times and oxidative balance, and in particular on stores of GSH.

Original languageEnglish
Pages (from-to)1249-1258
Number of pages10
JournalFree Radical Research
Volume39
Issue number11
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Quercetin
Flavonoids
Glutathione
Antioxidants
Oxidants
Reactive Oxygen Species
Cells
U937 Cells
Flow cytometry
Mitochondrial Membrane Potential
Phosphatidylserines
Cell membranes
Plasma Cells
Superoxides
Hydrogen Peroxide
Cell Survival
Flow Cytometry
Cell Membrane
Membranes
Cell Line

Keywords

  • Apoptosis
  • Flow cytometry
  • GSH
  • Mitochondrial membrane potential
  • Quercetin
  • ROS

ASJC Scopus subject areas

  • Biochemistry

Cite this

Essential requirement of reduced glutathione (GSH) for the anti-oxidant effect of the flavonoid quercetin. / Ferraresi, Roberta; Troiano, Leonarda; Roat, Erika; Lugli, Enrico; Nemes, Elisa; Nasi, Milena; Pinti, Marcello; Garcia Fernandez, Maria I.; Copper, Edwin L.; Cossarizza, Andrea.

In: Free Radical Research, Vol. 39, No. 11, 11.2005, p. 1249-1258.

Research output: Contribution to journalArticle

Ferraresi, R, Troiano, L, Roat, E, Lugli, E, Nemes, E, Nasi, M, Pinti, M, Garcia Fernandez, MI, Copper, EL & Cossarizza, A 2005, 'Essential requirement of reduced glutathione (GSH) for the anti-oxidant effect of the flavonoid quercetin', Free Radical Research, vol. 39, no. 11, pp. 1249-1258. https://doi.org/10.1080/10715760500306935
Ferraresi, Roberta ; Troiano, Leonarda ; Roat, Erika ; Lugli, Enrico ; Nemes, Elisa ; Nasi, Milena ; Pinti, Marcello ; Garcia Fernandez, Maria I. ; Copper, Edwin L. ; Cossarizza, Andrea. / Essential requirement of reduced glutathione (GSH) for the anti-oxidant effect of the flavonoid quercetin. In: Free Radical Research. 2005 ; Vol. 39, No. 11. pp. 1249-1258.
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