Essential role of caldesmon in the actin filament reorganization induced by glucocorticoids

Flora Castellino, Shoichiro Ono, Fumio Matsumura, Alberto Luini

Research output: Contribution to journalArticlepeer-review


Glucocorticoids induce the remodeling of the actin cytoskeleton and the formation of numerous stress fibers in a protein synthesis-dependent fashion in a variety of cell types (Castellino, F., J. Heuser, S. Marchetti, B. Bruno, and A. Luini. 1992. Proc. Natl. Acad. Sci. USA. 89:3775-3779). These cells can thus be used as models to investigate the mechanisms controlling the organization of actin filaments. Caldesmon is an almost ubiquitous actin- and calmodulin-binding protein that synergizes with tropomyosin to stabilize microfilaments in vitro (Matsumura, F., and Yamashiro, S. 1993. Current Opin. Cell Biol. 5:70-76). We now report that glucocorticoids (but not other steroids) enhanced the levels of caldesmon (both protein and mRNA) and induced the reorganization of microfilaments with similar time courses and potencies in A549 cells. A caldesmon antisense oligodeoxynucleotide targeted to the most abundant caldesmon isoform in A549 cells dramatically inhibited glucocorticoid-induced caldesmon synthesis and actin reorganization with similar potencies. Several control oligonucleotides were inactive. These results demonstrate that caldesmon has a crucial role in vivo in the organization of the actin cytoskeleton and suggest that hormone-induced changes in caldesmon levels mediate microfilament remodeling.

Original languageEnglish
Pages (from-to)1223-1230
Number of pages8
JournalJournal of Cell Biology
Issue number5
Publication statusPublished - Dec 1995

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Essential role of caldesmon in the actin filament reorganization induced by glucocorticoids'. Together they form a unique fingerprint.

Cite this