Essential role of caldesmon in the actin filament reorganization induced by glucocorticoids

Flora Castellino, Shoichiro Ono, Fumio Matsumura, Alberto Luini

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Glucocorticoids induce the remodeling of the actin cytoskeleton and the formation of numerous stress fibers in a protein synthesis-dependent fashion in a variety of cell types (Castellino, F., J. Heuser, S. Marchetti, B. Bruno, and A. Luini. 1992. Proc. Natl. Acad. Sci. USA. 89:3775-3779). These cells can thus be used as models to investigate the mechanisms controlling the organization of actin filaments. Caldesmon is an almost ubiquitous actin- and calmodulin-binding protein that synergizes with tropomyosin to stabilize microfilaments in vitro (Matsumura, F., and Yamashiro, S. 1993. Current Opin. Cell Biol. 5:70-76). We now report that glucocorticoids (but not other steroids) enhanced the levels of caldesmon (both protein and mRNA) and induced the reorganization of microfilaments with similar time courses and potencies in A549 cells. A caldesmon antisense oligodeoxynucleotide targeted to the most abundant caldesmon isoform in A549 cells dramatically inhibited glucocorticoid-induced caldesmon synthesis and actin reorganization with similar potencies. Several control oligonucleotides were inactive. These results demonstrate that caldesmon has a crucial role in vivo in the organization of the actin cytoskeleton and suggest that hormone-induced changes in caldesmon levels mediate microfilament remodeling.

Original languageEnglish
Pages (from-to)1223-1230
Number of pages8
JournalJournal of Cell Biology
Volume131
Issue number5
DOIs
Publication statusPublished - Dec 1995

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Calmodulin-Binding Proteins
Actin Cytoskeleton
Glucocorticoids
Microfilament Proteins
Stress Fibers
Tropomyosin
Oligodeoxyribonucleotides
Oligonucleotides
Actins
Protein Isoforms
Proteins
Steroids
Hormones
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Essential role of caldesmon in the actin filament reorganization induced by glucocorticoids. / Castellino, Flora; Ono, Shoichiro; Matsumura, Fumio; Luini, Alberto.

In: Journal of Cell Biology, Vol. 131, No. 5, 12.1995, p. 1223-1230.

Research output: Contribution to journalArticle

Castellino, Flora ; Ono, Shoichiro ; Matsumura, Fumio ; Luini, Alberto. / Essential role of caldesmon in the actin filament reorganization induced by glucocorticoids. In: Journal of Cell Biology. 1995 ; Vol. 131, No. 5. pp. 1223-1230.
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