TY - JOUR
T1 - Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base
T2 - Technical note
AU - Ricci-Vitiani, Lucia
AU - Pierconti, Francesco
AU - Falchetti, Maria Laura
AU - Petrucci, Giovanna
AU - Maira, Giulio
AU - De Maria, Ruggero
AU - Larocca, Luigi Maria
AU - Pallini, Roberto
PY - 2006
Y1 - 2006
N2 - Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.
AB - Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.
KW - Cell culture
KW - Chordoma
KW - Skull base tumor
KW - Telomerase
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U2 - 10.3171/jns.2006.105.3.482
DO - 10.3171/jns.2006.105.3.482
M3 - Article
C2 - 16961149
AN - SCOPUS:33748496013
VL - 105
SP - 482
EP - 484
JO - Journal of Neurosurgery
JF - Journal of Neurosurgery
SN - 0022-3085
IS - 3
ER -