Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base: Technical note

Lucia Ricci-Vitiani, Francesco Pierconti, Maria Laura Falchetti, Giovanna Petrucci, Giulio Maira, Ruggero De Maria, Luigi Maria Larocca, Roberto Pallini

Research output: Contribution to journalArticlepeer-review

Abstract

Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.

Original languageEnglish
Pages (from-to)482-484
Number of pages3
JournalJournal of Neurosurgery
Volume105
Issue number3
DOIs
Publication statusPublished - 2006

Keywords

  • Cell culture
  • Chordoma
  • Skull base tumor
  • Telomerase

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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