Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base: Technical note

Lucia Ricci-Vitiani; Francesco Pierconti; Maria Laura Falchetti; Giovanna Petrucci; Giulio Maira; Ruggero De Maria; Luigi Maria Larocca; Roberto Pallini

doi:10.3171/jns.2006.105.3.482

Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base: Technical note

Lucia Ricci-Vitiani, Francesco Pierconti, Maria Laura Falchetti, Giovanna Petrucci, Giulio Maira, Ruggero De Maria, Luigi Maria Larocca, Roberto Pallini

Research output: Contribution to journal › Article › peer-review

Abstract

Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.

Original language	English
Pages (from-to)	482-484
Number of pages	3
Journal	Journal of Neurosurgery
Volume	105
Issue number	3
DOIs	https://doi.org/10.3171/jns.2006.105.3.482
Publication status	Published - 2006

Keywords

Cell culture
Chordoma
Skull base tumor
Telomerase

ASJC Scopus subject areas

Clinical Neurology
Neuroscience(all)

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10.3171/jns.2006.105.3.482

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Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base : Technical note. / Ricci-Vitiani, Lucia; Pierconti, Francesco; Falchetti, Maria Laura; Petrucci, Giovanna; Maira, Giulio; De Maria, Ruggero; Larocca, Luigi Maria; Pallini, Roberto.

In: Journal of Neurosurgery, Vol. 105, No. 3, 2006, p. 482-484.

Research output: Contribution to journal › Article › peer-review

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AU - De Maria, Ruggero

AU - Larocca, Luigi Maria

AU - Pallini, Roberto

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AB - Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.

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Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base: Technical note

Abstract

Keywords

ASJC Scopus subject areas

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