Abstract
OBJECTIVES: Pancreatic cancer still remains a challenge for its biological complexity and lack of effective therapeutic strategies. Establishing new pancreatic cancer cell lines is therefore of paramount importance to clarify its biology. METHODS: We established and characterized 4 new pancreatic cancer cell lines (PP78, PP109, PP117, and PP161) according to their genetic (K-Ras, TP53, CDKN2A, and MADH4; DNA fingerprinting; karyotype), cytostructural (cytokeratins 7, 8, 18, and 19 vimentin, and ezrin), and functional profiles (doubling time; migration assay). RESULTS: K-Ras, TP53, and CDKN2A gene alterations were detected in all 4 of them. Each cell line had a unique DNA profile revealed by DNA fingerprinting. A complex karyotype with numerous structural and numeric chromosomal abnormalities was present in each cell line. All 4 cell lines showed positivity for cytokeratins 7, 8, and 18. All but PP78 expressed cytokeratin 19, whereas vimentin was expressed only in PP117 and PP78 cells. A different ezrin cellular distribution was noticed in PP78 and PP117, being mostly located at membrane ruffles. This peculiar distribution was associated with the strongest migratory capability. CONCLUSIONS: Our results seem to confirm the pancreatic ductal adenocarcinoma heterogeneity; in fact, the same genetic abnormalities (K-Ras, TP53, and CDKN2A) may have different effects on tumor biology depending on cellular differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 184-196 |
| Number of pages | 13 |
| Journal | Pancreas |
| Volume | 38 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 2009 |
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Keywords
- Cell lines
- Cytogenetic
- Ezrin
- Molecular genetics
- Pancreatic cancer
ASJC Scopus subject areas
- Hepatology
- Internal Medicine
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Establishment and characterization of 4 new human pancreatic cancer cell lines : Evidences of different tumor phenotypes. / Chifenti, Barbara; Morelli, Mariangela; Zavaglia, Michele; Coviello, Domenico A.; Guerneri, Silvana; Santucci, Annalisa; Paffetti, Alessandro; Masetti, Massimo; Locci, Maria Teresa; Bertacca, Gloria; Capodanno, Alessandra; Collecchi, Paola; Campani, Daniela; Mosca, Franco; Bevilacqua, Generoso; Cavazzana, Andrea O.
In: Pancreas, Vol. 38, No. 2, 03.2009, p. 184-196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment and characterization of 4 new human pancreatic cancer cell lines
T2 - Evidences of different tumor phenotypes
AU - Chifenti, Barbara
AU - Morelli, Mariangela
AU - Zavaglia, Michele
AU - Coviello, Domenico A.
AU - Guerneri, Silvana
AU - Santucci, Annalisa
AU - Paffetti, Alessandro
AU - Masetti, Massimo
AU - Locci, Maria Teresa
AU - Bertacca, Gloria
AU - Capodanno, Alessandra
AU - Collecchi, Paola
AU - Campani, Daniela
AU - Mosca, Franco
AU - Bevilacqua, Generoso
AU - Cavazzana, Andrea O.
PY - 2009/3
Y1 - 2009/3
N2 - OBJECTIVES: Pancreatic cancer still remains a challenge for its biological complexity and lack of effective therapeutic strategies. Establishing new pancreatic cancer cell lines is therefore of paramount importance to clarify its biology. METHODS: We established and characterized 4 new pancreatic cancer cell lines (PP78, PP109, PP117, and PP161) according to their genetic (K-Ras, TP53, CDKN2A, and MADH4; DNA fingerprinting; karyotype), cytostructural (cytokeratins 7, 8, 18, and 19 vimentin, and ezrin), and functional profiles (doubling time; migration assay). RESULTS: K-Ras, TP53, and CDKN2A gene alterations were detected in all 4 of them. Each cell line had a unique DNA profile revealed by DNA fingerprinting. A complex karyotype with numerous structural and numeric chromosomal abnormalities was present in each cell line. All 4 cell lines showed positivity for cytokeratins 7, 8, and 18. All but PP78 expressed cytokeratin 19, whereas vimentin was expressed only in PP117 and PP78 cells. A different ezrin cellular distribution was noticed in PP78 and PP117, being mostly located at membrane ruffles. This peculiar distribution was associated with the strongest migratory capability. CONCLUSIONS: Our results seem to confirm the pancreatic ductal adenocarcinoma heterogeneity; in fact, the same genetic abnormalities (K-Ras, TP53, and CDKN2A) may have different effects on tumor biology depending on cellular differentiation.
AB - OBJECTIVES: Pancreatic cancer still remains a challenge for its biological complexity and lack of effective therapeutic strategies. Establishing new pancreatic cancer cell lines is therefore of paramount importance to clarify its biology. METHODS: We established and characterized 4 new pancreatic cancer cell lines (PP78, PP109, PP117, and PP161) according to their genetic (K-Ras, TP53, CDKN2A, and MADH4; DNA fingerprinting; karyotype), cytostructural (cytokeratins 7, 8, 18, and 19 vimentin, and ezrin), and functional profiles (doubling time; migration assay). RESULTS: K-Ras, TP53, and CDKN2A gene alterations were detected in all 4 of them. Each cell line had a unique DNA profile revealed by DNA fingerprinting. A complex karyotype with numerous structural and numeric chromosomal abnormalities was present in each cell line. All 4 cell lines showed positivity for cytokeratins 7, 8, and 18. All but PP78 expressed cytokeratin 19, whereas vimentin was expressed only in PP117 and PP78 cells. A different ezrin cellular distribution was noticed in PP78 and PP117, being mostly located at membrane ruffles. This peculiar distribution was associated with the strongest migratory capability. CONCLUSIONS: Our results seem to confirm the pancreatic ductal adenocarcinoma heterogeneity; in fact, the same genetic abnormalities (K-Ras, TP53, and CDKN2A) may have different effects on tumor biology depending on cellular differentiation.
KW - Cell lines
KW - Cytogenetic
KW - Ezrin
KW - Molecular genetics
KW - Pancreatic cancer
UR - http://www.scopus.com/inward/record.url?scp=65349175891&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349175891&partnerID=8YFLogxK
U2 - 10.1097/MPA.0b013e31818c746a
DO - 10.1097/MPA.0b013e31818c746a
M3 - Article
VL - 38
SP - 184
EP - 196
JO - Pancreas
JF - Pancreas
SN - 0885-3177
IS - 2
ER -