TY - JOUR
T1 - Establishment and characterization of murine small cell lung carcinoma cell lines derived from HPV-16 E6/E7 transgenic mice
AU - Carraresi, Laura
AU - Martinelli, Rosanna
AU - Vannoni, Alessandro
AU - Riccio, Massimo
AU - Dembic, Maja
AU - Tripodi, Sergio
AU - Cintorino, Marcella
AU - Santi, Spartaco
AU - Bigliardi, Elisa
AU - Carmellini, Mario
AU - Rossini, Mara
PY - 2006/1/8
Y1 - 2006/1/8
N2 - We have established two murine cell lines derived from Small Cell Lung Carcinomas (SCLCs) developed by HPV-E6/E7 transgenic mice. These cells named PPAP-9 and PPAP-10 were isolated from mice bearing tumors, 9 and 10 months old, respectively. The cells, 5 μm in diameter, express HPV oncoproteins and sustain tumor formation after subcutaneous injection in syngenic mice. A detailed analysis indicated the epithelial origin and the neuroendocrine differentiation of these cells. We showed by confocal immunofluorescence the expression of the epithelial marker cytokeratin 5, whose gene promoter was used to direct the expression of HPV E6/E. Cells express several neuroendocrine markers such as CGRP, MAP-2, Ash1, CgrA, Scg2. The neuroendocrine differentiation of these cells was further confirmed by electron microscopy demonstrating neuropeptides secreting granules in their cytoplasm. Furthermore, in agreement with the altered expression observed in the majority of human SCLC we showed in these cells the absence of both p53 and pRB and a dramatic reduction in the expression of Caveolin-1.
AB - We have established two murine cell lines derived from Small Cell Lung Carcinomas (SCLCs) developed by HPV-E6/E7 transgenic mice. These cells named PPAP-9 and PPAP-10 were isolated from mice bearing tumors, 9 and 10 months old, respectively. The cells, 5 μm in diameter, express HPV oncoproteins and sustain tumor formation after subcutaneous injection in syngenic mice. A detailed analysis indicated the epithelial origin and the neuroendocrine differentiation of these cells. We showed by confocal immunofluorescence the expression of the epithelial marker cytokeratin 5, whose gene promoter was used to direct the expression of HPV E6/E. Cells express several neuroendocrine markers such as CGRP, MAP-2, Ash1, CgrA, Scg2. The neuroendocrine differentiation of these cells was further confirmed by electron microscopy demonstrating neuropeptides secreting granules in their cytoplasm. Furthermore, in agreement with the altered expression observed in the majority of human SCLC we showed in these cells the absence of both p53 and pRB and a dramatic reduction in the expression of Caveolin-1.
KW - Achaete-scute homologue 1
KW - Caveolin-1
KW - Chromogranin A
KW - Confocal immunofluorescence
KW - HPV-16 E6/E7 transgenic mice
KW - Microtubule-associated protein 2
KW - Murine cell lines
KW - Secretogranin 2
KW - SEM
KW - Small cell lung carcinoma
KW - TEM
UR - http://www.scopus.com/inward/record.url?scp=28844504699&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=28844504699&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2005.01.027
DO - 10.1016/j.canlet.2005.01.027
M3 - Article
C2 - 16356832
AN - SCOPUS:28844504699
VL - 231
SP - 65
EP - 73
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -