Establishment and characterization of three new cell lines derived from the ascites of human ovarian carcinomas

Angela Alama, Federica Barbieri, Anna Favre, Monica Cagnoli, Elvira Noviello, Franco Pedullà, Maurizio Viale, Gianni Foglia, Nicola Ragni

Research output: Contribution to journalArticle

Abstract

Three human cancer cell lines (OC 314, OC 315, and OC 316) were newly established in permanent culture from the ascites of patients with serous adenocarcinoma of the ovary. OC 314 was derived from an untreated tumor presenting with ascites at diagnosis; OC 315 was isolated from a neoplasm progressing after cisplatin-containing regimen; and OC 316 was collected from a patient with pleural metastasis at diagnosis, resistant to different chemotherapeutic treatments including Taxol. These cell lines were repetitively subcultured once to twice a week through 7580 passage generations. Tumor cells grew as monolayers and displayed epithelial-like morphology, consistent with a feature of adenocarcinoma, which was then confirmed by the expressions of cytokeratins and vimentin. The cell lines proved highly tumorigenic when transplanted into nude mice, both subcutaneously and intraperitoneally. In addition, the mice inoculated with subcutaneous OC 316 developed extremely aggressive tumor, also invading the peritoneum, which correlated with the malignant behavior of the original tumor. Drug sensitivity, evaluated by the MTT assay, showed that the three cell lines expressed similar sensitivity to doxorubicin. Responses to cisplatin essentially reported low sensitivity of OC 314 and OC 315 and resistance of OC 316, thus reflecting the original sensitivity at the clinical level.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalGynecologic Oncology
Volume62
Issue number1
DOIs
Publication statusPublished - Jul 1996

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

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