Establishment and characterization of xenografts and cancer cell cultures derived from BRCA1 -/- epithelial ovarian cancers

Stefano Indraccolo, Veronica Tisato, Simona Agata, Lidia Moserle, Silvia Ferrari, Monia Callegaro, Luca Persano, Maurizia Dalla Palma, Maria Chiara Scaini, Giovanni Esposito, Ambrogio Fassina, Ornella Nicoletto, Mario Plebani, Luigi Chieco-Bianchi, Alberto Amadori, Emma D'Andrea, Marco Montagna

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The BRCA1 gene is responsible for a high number of hereditary breast and ovarian cancers that cluster in families with a strong genetic predisposition. Despite intense investigation, the accumulating findings on BRCA1 biological functions have not yet been translated into specific therapeutic approaches, also due to the lack of suitable experimental models. The purpose of this study was to establish and characterize cell cultures and xenografts from patients with BRCA1 -/- ovarian cancers. We derived two ovarian cancer cell lines, termed PD-OVCA1 and PD-OVCA2, both from patients previously treated with chemotherapy, that propagate in SCID mice as well as in vitro for a limited number of passages. Both cell lines expressed cytokeratins and the CA125 tumour marker. A detailed molecular characterization highlighted both constitutive and somatic genetic events that abrogate BRCA1 gene function. Both cell lines were shown to lose the wild type BRCA1 allele; intriguingly, these deletions were apparently accompanied by gain of one or more copies of the mutant alleles. Finally, a genomic profile of major chromosomal aberrations was obtained by the Multiplex Ligation-dependent Probe Amplification (MLPA) technique, which disclosed chromosomal imbalances targeting specific genes in each cell line. The PD-OVCA1 and PD-OVCA2 ovarian cancer cell lines will provide a valuable tool for new experimental models for the study of BRCA1-associated tumour biology.

Original languageEnglish
Pages (from-to)1475-1483
Number of pages9
JournalEuropean Journal of Cancer
Volume42
Issue number10
DOIs
Publication statusPublished - Jul 2006

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Heterografts
Cell Culture Techniques
Ovarian Neoplasms
Cell Line
BRCA1 Gene
Neoplasms
Theoretical Models
Alleles
SCID Mice
Gene Targeting
Multiplex Polymerase Chain Reaction
Genetic Predisposition to Disease
Tumor Biomarkers
Keratins
Chromosome Aberrations
Ovarian epithelial cancer
Breast Neoplasms
Drug Therapy
Therapeutics

Keywords

  • BRCA1
  • CA125
  • MLPA
  • Ovarian cancer
  • Xenograft

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Establishment and characterization of xenografts and cancer cell cultures derived from BRCA1 -/- epithelial ovarian cancers. / Indraccolo, Stefano; Tisato, Veronica; Agata, Simona; Moserle, Lidia; Ferrari, Silvia; Callegaro, Monia; Persano, Luca; Palma, Maurizia Dalla; Scaini, Maria Chiara; Esposito, Giovanni; Fassina, Ambrogio; Nicoletto, Ornella; Plebani, Mario; Chieco-Bianchi, Luigi; Amadori, Alberto; D'Andrea, Emma; Montagna, Marco.

In: European Journal of Cancer, Vol. 42, No. 10, 07.2006, p. 1475-1483.

Research output: Contribution to journalArticle

Indraccolo, S, Tisato, V, Agata, S, Moserle, L, Ferrari, S, Callegaro, M, Persano, L, Palma, MD, Scaini, MC, Esposito, G, Fassina, A, Nicoletto, O, Plebani, M, Chieco-Bianchi, L, Amadori, A, D'Andrea, E & Montagna, M 2006, 'Establishment and characterization of xenografts and cancer cell cultures derived from BRCA1 -/- epithelial ovarian cancers', European Journal of Cancer, vol. 42, no. 10, pp. 1475-1483. https://doi.org/10.1016/j.ejca.2006.01.057
Indraccolo, Stefano ; Tisato, Veronica ; Agata, Simona ; Moserle, Lidia ; Ferrari, Silvia ; Callegaro, Monia ; Persano, Luca ; Palma, Maurizia Dalla ; Scaini, Maria Chiara ; Esposito, Giovanni ; Fassina, Ambrogio ; Nicoletto, Ornella ; Plebani, Mario ; Chieco-Bianchi, Luigi ; Amadori, Alberto ; D'Andrea, Emma ; Montagna, Marco. / Establishment and characterization of xenografts and cancer cell cultures derived from BRCA1 -/- epithelial ovarian cancers. In: European Journal of Cancer. 2006 ; Vol. 42, No. 10. pp. 1475-1483.
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AU - D'Andrea, Emma

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