Establishment and genetic characterization of ANGM-CSS, a novel, immortal cell line derived from a human glioblastoma multiforme

Angelantonio Notarangelo, Domenico Trombetta, Vincenzo D'Angelo, Paola Parrella, Orazio Palumbo, Clelia Tiziana Storlazzi, Luciana Impera, Lucia Anna Muscarella, Antonella La Torre, Andrea Affuso, Vito Michele Fazio, Massimo Carella, Leopoldo Zelante

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Glioblastoma multiforme (World Health Organization, grade IV astrocytoma) is the most common and most aggressive malignant primary brain tumor. We report a novel cell line, designated as ANGM-CSS, which was established from a 56-year-old male patient with a surgically removed glioblastoma multiforme. The ANGM-CSS cell line was established in vitro and characterized using histological and immunohistochemical staining, classical and molecular cytogenetic analyses, molecular studies and functional assays using a xenograft model in immunodeficient animals. ANGM-CSS was positive for CD133, nestin and vimentin proteins, whereas GFAP showed staining only in a fraction of the cells. Cytogenetic and molecular cytogenetic analysis revealed a near-tetraploid karyotype, with a modal chromosome number from 88 to 91, and additional cytogenetic abnormalities, such as the t(6;14)(p12;q11.2), t(8;10)(q24.2;q21.1) and t(5;9) (q34;p21) unbalanced translocations. Moreover, ANGM-CSS showed amplification of the MET and EGFR genes whose overexpression was observed at the mRNA level. Interestingly, ANGM-CSS is tumorigenic when implanted in immunodeficient mice, and the cells obtained from the xenografts showed the same morphology and karyotype in vitro as the original cell line. ANGM-CSS represents a biologically relevant cell line to be used to investigate the molecular pathology of glioblastoma multiforme, also to evaluate the efficacy of novel therapeutic drugs in vitro.

Original languageEnglish
Pages (from-to)717-724
Number of pages8
JournalInternational Journal of Oncology
Volume44
Issue number3
DOIs
Publication statusPublished - Mar 2014

Fingerprint

Glioblastoma
Cell Line
Cytogenetic Analysis
Karyotype
Heterografts
Staining and Labeling
erbB-1 Genes
Nestin
Tetraploidy
Molecular Pathology
Vimentin
Brain Neoplasms
Cytogenetics
Chromosome Aberrations
Chromosomes
Messenger RNA
Pharmaceutical Preparations
In Vitro Techniques
Therapeutics

Keywords

  • Cytogenetics
  • Fluorescent in situ hybridization
  • Glioblastoma cell line
  • In vitro test system

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Establishment and genetic characterization of ANGM-CSS, a novel, immortal cell line derived from a human glioblastoma multiforme. / Notarangelo, Angelantonio; Trombetta, Domenico; D'Angelo, Vincenzo; Parrella, Paola; Palumbo, Orazio; Tiziana Storlazzi, Clelia; Impera, Luciana; Muscarella, Lucia Anna; La Torre, Antonella; Affuso, Andrea; Fazio, Vito Michele; Carella, Massimo; Zelante, Leopoldo.

In: International Journal of Oncology, Vol. 44, No. 3, 03.2014, p. 717-724.

Research output: Contribution to journalArticle

Notarangelo, Angelantonio ; Trombetta, Domenico ; D'Angelo, Vincenzo ; Parrella, Paola ; Palumbo, Orazio ; Tiziana Storlazzi, Clelia ; Impera, Luciana ; Muscarella, Lucia Anna ; La Torre, Antonella ; Affuso, Andrea ; Fazio, Vito Michele ; Carella, Massimo ; Zelante, Leopoldo. / Establishment and genetic characterization of ANGM-CSS, a novel, immortal cell line derived from a human glioblastoma multiforme. In: International Journal of Oncology. 2014 ; Vol. 44, No. 3. pp. 717-724.
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AU - Notarangelo, Angelantonio

AU - Trombetta, Domenico

AU - D'Angelo, Vincenzo

AU - Parrella, Paola

AU - Palumbo, Orazio

AU - Tiziana Storlazzi, Clelia

AU - Impera, Luciana

AU - Muscarella, Lucia Anna

AU - La Torre, Antonella

AU - Affuso, Andrea

AU - Fazio, Vito Michele

AU - Carella, Massimo

AU - Zelante, Leopoldo

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N2 - Glioblastoma multiforme (World Health Organization, grade IV astrocytoma) is the most common and most aggressive malignant primary brain tumor. We report a novel cell line, designated as ANGM-CSS, which was established from a 56-year-old male patient with a surgically removed glioblastoma multiforme. The ANGM-CSS cell line was established in vitro and characterized using histological and immunohistochemical staining, classical and molecular cytogenetic analyses, molecular studies and functional assays using a xenograft model in immunodeficient animals. ANGM-CSS was positive for CD133, nestin and vimentin proteins, whereas GFAP showed staining only in a fraction of the cells. Cytogenetic and molecular cytogenetic analysis revealed a near-tetraploid karyotype, with a modal chromosome number from 88 to 91, and additional cytogenetic abnormalities, such as the t(6;14)(p12;q11.2), t(8;10)(q24.2;q21.1) and t(5;9) (q34;p21) unbalanced translocations. Moreover, ANGM-CSS showed amplification of the MET and EGFR genes whose overexpression was observed at the mRNA level. Interestingly, ANGM-CSS is tumorigenic when implanted in immunodeficient mice, and the cells obtained from the xenografts showed the same morphology and karyotype in vitro as the original cell line. ANGM-CSS represents a biologically relevant cell line to be used to investigate the molecular pathology of glioblastoma multiforme, also to evaluate the efficacy of novel therapeutic drugs in vitro.

AB - Glioblastoma multiforme (World Health Organization, grade IV astrocytoma) is the most common and most aggressive malignant primary brain tumor. We report a novel cell line, designated as ANGM-CSS, which was established from a 56-year-old male patient with a surgically removed glioblastoma multiforme. The ANGM-CSS cell line was established in vitro and characterized using histological and immunohistochemical staining, classical and molecular cytogenetic analyses, molecular studies and functional assays using a xenograft model in immunodeficient animals. ANGM-CSS was positive for CD133, nestin and vimentin proteins, whereas GFAP showed staining only in a fraction of the cells. Cytogenetic and molecular cytogenetic analysis revealed a near-tetraploid karyotype, with a modal chromosome number from 88 to 91, and additional cytogenetic abnormalities, such as the t(6;14)(p12;q11.2), t(8;10)(q24.2;q21.1) and t(5;9) (q34;p21) unbalanced translocations. Moreover, ANGM-CSS showed amplification of the MET and EGFR genes whose overexpression was observed at the mRNA level. Interestingly, ANGM-CSS is tumorigenic when implanted in immunodeficient mice, and the cells obtained from the xenografts showed the same morphology and karyotype in vitro as the original cell line. ANGM-CSS represents a biologically relevant cell line to be used to investigate the molecular pathology of glioblastoma multiforme, also to evaluate the efficacy of novel therapeutic drugs in vitro.

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