Establishment and growth regulation of a novel ovarian cancer cell line from a poorly-differentiated adenocarcinoma: Proposal for a new treatment

Aldo Mancini, A. Borrelli, S. Formisano, M. T. Masucci, A. Maffeo, G. Perla, L. De Martino, N. Bevilacqua, G. Botti, T. Maggino

Research output: Contribution to journalArticlepeer-review

Abstract

A continuously growing cultured cell line has been obtained in vitro, starting from a specimen of ascites fluid obtained from a patient with ovarian cancer, in whom a poorly-differentiated adenocarcinoma was diagnosed. This cell line, named OC-A1, is routinely grown in standard, serum-supplemented culture medium and has been fully stabilized to long-term growth and characterized for both cultural and genetic parameters. OC-A1 cells express a set of characteristics, as determined in vitro which, when compared with the in vitro primary tumor, confirm the high malignity of this cancer. In addition, karyotype analysis showed a translocation of chromosome 8 which is correlated with the amplification of c-myc oncogene. However, the expression of this oncogene was found to be significantly inhibited by a new regulatory activity, recently found to be present in a liposarcoma cell line. Conditioned medium from these cells was indeed able to inhibit the growth of CC-A1 cells, arresting their cell cycle in the G1 phase and inducing them to apoptosis. Finally, the cell programmed death appeared to be related to the expression of antioncogene p53.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalEuropean Journal of Gynaecological Oncology
Volume20
Issue number1
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Oncology

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