Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality

Corrado Tarella, Patrizia Pregno, Eugenio Gallo, Daniele Caracciolo, Silvia Stefani, Cosimo Barletta, Luisa Lanfrancone, Dario Ferrero

Research output: Contribution to journalArticlepeer-review

Abstract

Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-μ-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalLeukemia Research
Volume14
Issue number2
DOIs
Publication statusPublished - 1990

Keywords

  • Adult ALL
  • autocrinism
  • B-cell lines
  • tumor progression

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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