Abstract
Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-μ-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.
Original language | English |
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Pages (from-to) | 177-184 |
Number of pages | 8 |
Journal | Leukemia Research |
Volume | 14 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1990 |
Keywords
- Adult ALL
- autocrinism
- B-cell lines
- tumor progression
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology