Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality

Corrado Tarella; Patrizia Pregno; Eugenio Gallo; Daniele Caracciolo; Silvia Stefani; Cosimo Barletta; Luisa Lanfrancone; Dario Ferrero

doi:10.1016/0145-2126(90)90047-D

Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality

Corrado Tarella, Patrizia Pregno, Eugenio Gallo, Daniele Caracciolo, Silvia Stefani, Cosimo Barletta, Luisa Lanfrancone, Dario Ferrero

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article › peer-review

Abstract

Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-μ-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.

Original language	English
Pages (from-to)	177-184
Number of pages	8
Journal	Leukemia Research
Volume	14
Issue number	2
DOIs	https://doi.org/10.1016/0145-2126(90)90047-D
Publication status	Published - 1990

Keywords

Adult ALL
autocrinism
B-cell lines
tumor progression

ASJC Scopus subject areas

Cancer Research
Hematology
Oncology

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10.1016/0145-2126(90)90047-D

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title = "Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality",

abstract = "Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-μ-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.",

keywords = "Adult ALL, autocrinism, B-cell lines, tumor progression",

author = "Corrado Tarella and Patrizia Pregno and Eugenio Gallo and Daniele Caracciolo and Silvia Stefani and Cosimo Barletta and Luisa Lanfrancone and Dario Ferrero",

year = "1990",

doi = "10.1016/0145-2126(90)90047-D",

language = "English",

volume = "14",

pages = "177--184",

journal = "Leukemia Research",

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publisher = "Elsevier Ltd",

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T1 - Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality

AU - Tarella, Corrado

AU - Pregno, Patrizia

AU - Gallo, Eugenio

AU - Caracciolo, Daniele

AU - Stefani, Silvia

AU - Barletta, Cosimo

AU - Lanfrancone, Luisa

AU - Ferrero, Dario

PY - 1990

Y1 - 1990

N2 - Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-μ-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.

AB - Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-μ-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.

KW - Adult ALL

KW - autocrinism

KW - B-cell lines

KW - tumor progression

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JF - Leukemia Research

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Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality

Abstract

Keywords

ASJC Scopus subject areas

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