Establishment of HHV-8-positive and HHV-8-negative lymphoma cell lines from primary lymphomatous effusions

Antonino Carbone, Anna M. Cilia, Annunziata Gloghini, Vincenzo Canzonieri, Cristina Pastore, Michela Todesco, Marzia Cozzi, Tiziana Perin, K. Rachele Volpe, Antonio Pinto, Gianluca Gaidano

Research output: Contribution to journalArticle

Abstract

Primary lymphomatous effusions are represented by cases of non-Hodgkin's lymphoma (NHL) which grow in liquid phase in the serous body cavities in the absence of solid tumour masses. Based on morphologic, immunophenotypic, virologic and genotypic features, primary lymphomatous effusions are distinguished into body cavity-based lymphoma (BCBL), Burkitt's lymphoma (BL) and immunoblastic large-cell lymphoma. The histogenesis and pathogenesis of primary lymphomatous effusions are virtually unclarified. In this study, we have established 2 cell lines (CRO-AP/I and CRO-AP/2) representative of 2 distinct categories of primary lymphomatous effusion, BCBL (CRO-AP/2) and BL (CRO-AP/I). Both CRO-AP/I and CRO-AP/2 carry monoclonal re-arrangements of the immunoglobulin genes which are identical to those of the respective parental tumours. Consistent with its BCBL origin, CRO-AP/2 is characterised by a non-B, non-T phenotype and harbours infection by HHY-8 (approx. 100 vital copies/cell) and Epstein-Barr virus. Conversely, CROAP/I expresses several B cell-associated antigens, lacks HHV-8 infection and carries the genetic hallmark of BL, i.e., a chromosomal breakpoint of band 8q24. CRO- AP/I and CROAPI2 may be valuable for the biologic characterization of primary lymphomatous effusions, particularly since the number of available cell lines derived from such lymphomatous effusions is extremely limited.

Original languageEnglish
Pages (from-to)562-569
Number of pages8
JournalInternational Journal of Cancer
Volume73
Issue number4
DOIs
Publication statusPublished - 1997

Fingerprint

Human Herpesvirus 8
Burkitt Lymphoma
Lymphoma
Cell Line
Large-Cell Immunoblastic Lymphoma
Immunoglobulin Genes
Infection
Human Herpesvirus 4
Non-Hodgkin's Lymphoma
Neoplasms
B-Lymphocytes
Phenotype
Antigens

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Establishment of HHV-8-positive and HHV-8-negative lymphoma cell lines from primary lymphomatous effusions. / Carbone, Antonino; Cilia, Anna M.; Gloghini, Annunziata; Canzonieri, Vincenzo; Pastore, Cristina; Todesco, Michela; Cozzi, Marzia; Perin, Tiziana; Volpe, K. Rachele; Pinto, Antonio; Gaidano, Gianluca.

In: International Journal of Cancer, Vol. 73, No. 4, 1997, p. 562-569.

Research output: Contribution to journalArticle

Carbone, Antonino ; Cilia, Anna M. ; Gloghini, Annunziata ; Canzonieri, Vincenzo ; Pastore, Cristina ; Todesco, Michela ; Cozzi, Marzia ; Perin, Tiziana ; Volpe, K. Rachele ; Pinto, Antonio ; Gaidano, Gianluca. / Establishment of HHV-8-positive and HHV-8-negative lymphoma cell lines from primary lymphomatous effusions. In: International Journal of Cancer. 1997 ; Vol. 73, No. 4. pp. 562-569.
@article{356a44004e944da7b9262f327ac788b9,
title = "Establishment of HHV-8-positive and HHV-8-negative lymphoma cell lines from primary lymphomatous effusions",
abstract = "Primary lymphomatous effusions are represented by cases of non-Hodgkin's lymphoma (NHL) which grow in liquid phase in the serous body cavities in the absence of solid tumour masses. Based on morphologic, immunophenotypic, virologic and genotypic features, primary lymphomatous effusions are distinguished into body cavity-based lymphoma (BCBL), Burkitt's lymphoma (BL) and immunoblastic large-cell lymphoma. The histogenesis and pathogenesis of primary lymphomatous effusions are virtually unclarified. In this study, we have established 2 cell lines (CRO-AP/I and CRO-AP/2) representative of 2 distinct categories of primary lymphomatous effusion, BCBL (CRO-AP/2) and BL (CRO-AP/I). Both CRO-AP/I and CRO-AP/2 carry monoclonal re-arrangements of the immunoglobulin genes which are identical to those of the respective parental tumours. Consistent with its BCBL origin, CRO-AP/2 is characterised by a non-B, non-T phenotype and harbours infection by HHY-8 (approx. 100 vital copies/cell) and Epstein-Barr virus. Conversely, CROAP/I expresses several B cell-associated antigens, lacks HHV-8 infection and carries the genetic hallmark of BL, i.e., a chromosomal breakpoint of band 8q24. CRO- AP/I and CROAPI2 may be valuable for the biologic characterization of primary lymphomatous effusions, particularly since the number of available cell lines derived from such lymphomatous effusions is extremely limited.",
author = "Antonino Carbone and Cilia, {Anna M.} and Annunziata Gloghini and Vincenzo Canzonieri and Cristina Pastore and Michela Todesco and Marzia Cozzi and Tiziana Perin and Volpe, {K. Rachele} and Antonio Pinto and Gianluca Gaidano",
year = "1997",
doi = "10.1002/(SICI)1097-0215(19971114)73:4<562::AID-IJC18>3.0.CO;2-B",
language = "English",
volume = "73",
pages = "562--569",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Establishment of HHV-8-positive and HHV-8-negative lymphoma cell lines from primary lymphomatous effusions

AU - Carbone, Antonino

AU - Cilia, Anna M.

AU - Gloghini, Annunziata

AU - Canzonieri, Vincenzo

AU - Pastore, Cristina

AU - Todesco, Michela

AU - Cozzi, Marzia

AU - Perin, Tiziana

AU - Volpe, K. Rachele

AU - Pinto, Antonio

AU - Gaidano, Gianluca

PY - 1997

Y1 - 1997

N2 - Primary lymphomatous effusions are represented by cases of non-Hodgkin's lymphoma (NHL) which grow in liquid phase in the serous body cavities in the absence of solid tumour masses. Based on morphologic, immunophenotypic, virologic and genotypic features, primary lymphomatous effusions are distinguished into body cavity-based lymphoma (BCBL), Burkitt's lymphoma (BL) and immunoblastic large-cell lymphoma. The histogenesis and pathogenesis of primary lymphomatous effusions are virtually unclarified. In this study, we have established 2 cell lines (CRO-AP/I and CRO-AP/2) representative of 2 distinct categories of primary lymphomatous effusion, BCBL (CRO-AP/2) and BL (CRO-AP/I). Both CRO-AP/I and CRO-AP/2 carry monoclonal re-arrangements of the immunoglobulin genes which are identical to those of the respective parental tumours. Consistent with its BCBL origin, CRO-AP/2 is characterised by a non-B, non-T phenotype and harbours infection by HHY-8 (approx. 100 vital copies/cell) and Epstein-Barr virus. Conversely, CROAP/I expresses several B cell-associated antigens, lacks HHV-8 infection and carries the genetic hallmark of BL, i.e., a chromosomal breakpoint of band 8q24. CRO- AP/I and CROAPI2 may be valuable for the biologic characterization of primary lymphomatous effusions, particularly since the number of available cell lines derived from such lymphomatous effusions is extremely limited.

AB - Primary lymphomatous effusions are represented by cases of non-Hodgkin's lymphoma (NHL) which grow in liquid phase in the serous body cavities in the absence of solid tumour masses. Based on morphologic, immunophenotypic, virologic and genotypic features, primary lymphomatous effusions are distinguished into body cavity-based lymphoma (BCBL), Burkitt's lymphoma (BL) and immunoblastic large-cell lymphoma. The histogenesis and pathogenesis of primary lymphomatous effusions are virtually unclarified. In this study, we have established 2 cell lines (CRO-AP/I and CRO-AP/2) representative of 2 distinct categories of primary lymphomatous effusion, BCBL (CRO-AP/2) and BL (CRO-AP/I). Both CRO-AP/I and CRO-AP/2 carry monoclonal re-arrangements of the immunoglobulin genes which are identical to those of the respective parental tumours. Consistent with its BCBL origin, CRO-AP/2 is characterised by a non-B, non-T phenotype and harbours infection by HHY-8 (approx. 100 vital copies/cell) and Epstein-Barr virus. Conversely, CROAP/I expresses several B cell-associated antigens, lacks HHV-8 infection and carries the genetic hallmark of BL, i.e., a chromosomal breakpoint of band 8q24. CRO- AP/I and CROAPI2 may be valuable for the biologic characterization of primary lymphomatous effusions, particularly since the number of available cell lines derived from such lymphomatous effusions is extremely limited.

UR - http://www.scopus.com/inward/record.url?scp=18844472706&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18844472706&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19971114)73:4<562::AID-IJC18>3.0.CO;2-B

DO - 10.1002/(SICI)1097-0215(19971114)73:4<562::AID-IJC18>3.0.CO;2-B

M3 - Article

C2 - 9389573

AN - SCOPUS:18844472706

VL - 73

SP - 562

EP - 569

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -