Estimation of PHA induced transformation in peripheral blood lymphocytes through measurement of their increased glycolysis. An enzymatic micromethod

P. Cordiali-Fei, A. Floridi, M. C. Apollonj Ghetti, P. G. Natali

Research output: Contribution to journalArticle

Abstract

Peripheral blood lymphocytes undergoing blast transformation after contact with PHA, show a rapid increase in glycolysis. The phenomenon can be quantitated by measuring the amount of lactate released in the culture medium. This measurement has been adapted to a micromethod which allows the study of PHA induced transformation in a large number of samples in a short time. The present method which avoids the use of radioisotopes correlates in 90% of the cases with the 3H-thymidine incorporation test.

Original languageEnglish
Pages (from-to)389-401
Number of pages13
JournalImmunological Communications
Volume9
Issue number4
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Immunology

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