Estrogens maintain bile duct mass and reduce apoptosis after biliodigestive anastomosis in bile duct ligated rats

Gianluca Svegliati-Baroni, Roberto Ghiselli, Marco Marzioni, Domenico Alvaro, Federico Mocchegiani, Stefania Saccomanno, Valerio Sisti, Laura Ugili, Fiorenza Orlando, Gianfranco Alpini, Vittorio Saba, Antonio Benedetti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background/Aims: Disapperacence of bile ducts (ductopenia) represents the terminal, common stage of human cholangiopathies, and estrogens exert a major role in stimulating cholangiocyte proliferation. We thus evaluated whether estrogen administration protect from the bile duct loss induced by the biliary-digestive diversion in bile duct ligated (BDL) rats. Methods: After 3 weeks of BDL, rats were subjected to biliary-digestive diversion and treated with daily injections of 17β-estradiol or a control solution. Results: Both after 7 and 14 days from the biliary-digestive diversion a marked increase of the number of apoptotic cholangiocytes was observed. In contrast, 17β-estradiol significantly reduced cholangiocyte apoptosis. 17β-estradiol also prevented the biliary-digestive diversion-induced loss of PCNA-positive cholangiocytes and of the bile duct mass. Biliary-digestive diversion determined a marked reduction of ERK1/2 phopsphorylation in cholangiocytes that was reversed by the administration of 17β-estradiol. Conclusions: This study indicates that estrogens prevent the increase of cholangiocyte apoptosis and loss of cholangiocyte proliferation induced by the biliary-digestive diversion in the BDL rat. In parallel, 17β-estradiol also enhanced ERK1/2 phosphorylation, which is instead strongly reduced by the biliary-digestive diversion. These novel findings suggest that estrogens could prevent the evolution of cholangiopathies toward ductopenia.

Original languageEnglish
Pages (from-to)1158-1166
Number of pages9
JournalJournal of Hepatology
Volume44
Issue number6
DOIs
Publication statusPublished - Jun 2006

Fingerprint

Bile
Estradiol
Estrogens
Apoptosis
Bile Ducts
Proliferating Cell Nuclear Antigen
Phosphorylation
Injections

Keywords

  • Cholangiocyte
  • Cholestasis
  • Proliferation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Svegliati-Baroni, G., Ghiselli, R., Marzioni, M., Alvaro, D., Mocchegiani, F., Saccomanno, S., ... Benedetti, A. (2006). Estrogens maintain bile duct mass and reduce apoptosis after biliodigestive anastomosis in bile duct ligated rats. Journal of Hepatology, 44(6), 1158-1166. https://doi.org/10.1016/j.jhep.2005.10.032

Estrogens maintain bile duct mass and reduce apoptosis after biliodigestive anastomosis in bile duct ligated rats. / Svegliati-Baroni, Gianluca; Ghiselli, Roberto; Marzioni, Marco; Alvaro, Domenico; Mocchegiani, Federico; Saccomanno, Stefania; Sisti, Valerio; Ugili, Laura; Orlando, Fiorenza; Alpini, Gianfranco; Saba, Vittorio; Benedetti, Antonio.

In: Journal of Hepatology, Vol. 44, No. 6, 06.2006, p. 1158-1166.

Research output: Contribution to journalArticle

Svegliati-Baroni, G, Ghiselli, R, Marzioni, M, Alvaro, D, Mocchegiani, F, Saccomanno, S, Sisti, V, Ugili, L, Orlando, F, Alpini, G, Saba, V & Benedetti, A 2006, 'Estrogens maintain bile duct mass and reduce apoptosis after biliodigestive anastomosis in bile duct ligated rats', Journal of Hepatology, vol. 44, no. 6, pp. 1158-1166. https://doi.org/10.1016/j.jhep.2005.10.032
Svegliati-Baroni G, Ghiselli R, Marzioni M, Alvaro D, Mocchegiani F, Saccomanno S et al. Estrogens maintain bile duct mass and reduce apoptosis after biliodigestive anastomosis in bile duct ligated rats. Journal of Hepatology. 2006 Jun;44(6):1158-1166. https://doi.org/10.1016/j.jhep.2005.10.032
Svegliati-Baroni, Gianluca ; Ghiselli, Roberto ; Marzioni, Marco ; Alvaro, Domenico ; Mocchegiani, Federico ; Saccomanno, Stefania ; Sisti, Valerio ; Ugili, Laura ; Orlando, Fiorenza ; Alpini, Gianfranco ; Saba, Vittorio ; Benedetti, Antonio. / Estrogens maintain bile duct mass and reduce apoptosis after biliodigestive anastomosis in bile duct ligated rats. In: Journal of Hepatology. 2006 ; Vol. 44, No. 6. pp. 1158-1166.
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