Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds

Chiara Gualandi, Marco Govoni, Laura Foroni, Sabrina Valente, Michele Bianchi, Emanuele Giordano, Gianandrea Pasquinelli, Fabio Biscarini, Maria Letizia Focarete

Research output: Contribution to journalArticle

Abstract

Disinfection of electrospun materials is commonly carried out by ethanol (EtOH) soaking. However, in the literature no systematic investigation on the effect of EtOH disinfection on scaffold properties is available. In this study electrospun poly(l-lactic acid) (PLLA) scaffolds were differently treated in EtOH and changes of fiber morphology, fiber surface topography and scaffold thermo-mechanical properties were evaluated. It was found that EtOH induced the formation of an amount of crystal phase, in the initially amorphous fibers, which depends on EtOH grade, soaking time and temperature. Completely amorphous PLLA (a-PLLA) and semicrystalline PLLA (sc-PLLA) scaffolds were produced by applying different EtOH treatments to as-spun PLLA scaffold. Compared to a-PLLA, sc-PLLA was stiffer and composed of fibers with rougher surface. Cell culture performed by using permanent cell line H9c2 demonstrated that changes of scaffold properties determined a different cell response, in particular in the expression of proteins correlated to cell motility and cell adhesion.

Original languageEnglish
Pages (from-to)2008-2018
Number of pages11
JournalEuropean Polymer Journal
Volume48
Issue number12
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Crystallinity
  • Electrospinning
  • H9c2 cells
  • Polylactic acid
  • Sterilization

ASJC Scopus subject areas

  • Polymers and Plastics
  • Physics and Astronomy(all)
  • Organic Chemistry

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  • Cite this

    Gualandi, C., Govoni, M., Foroni, L., Valente, S., Bianchi, M., Giordano, E., Pasquinelli, G., Biscarini, F., & Focarete, M. L. (2012). Ethanol disinfection affects physical properties and cell response of electrospun poly(l-lactic acid) scaffolds. European Polymer Journal, 48(12), 2008-2018. https://doi.org/10.1016/j.eurpolymj.2012.09.016