Ethanol-induced changes of intracellular thiol compartmentation and protein redox status in the rat liver: Effect of tauroursodeoxycholate

Gianluigi Vendemiale, Ignazio Grattagliano, Anna Signorile, Emanuele Altomare

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Abstract

Background/Aims: Ethanol impairs cellular antioxidant defense and protein metabolism. Hydrophilic bile acids are protective against ethanol- induced cytotoxicity. This study investigated the compartmentation of intracellular thiol and protein redox status after acute ethanol intoxication in the liver and the effect of tauroursodeoxycholate pretreatment. Methods: The concentrations of total glutathione, glutathione bound to proteins, sulfhydryl proteins, carbonyl proteins and malondialdehyde were measured in hepatic cytosol, mitochondria and nuclei after oral administration of 25% ethanol (4 g/kg) or isocaloric carbohydrate solution to rats. The metabolisms of ethanol and acetaldehyde were investigated by giving 4-methylpyrazole (1 mmol/kg i.p.) or cyanamide (15 mg/kg i.p.) 1 h prior to ethanol ingestion. One group of rats received tauroursodeoxycholate (12 mg/kg p.os) 1 h before ethanol ingestion. Results: Ethanol significantly decreased the glutathione concentrations. Significant increases in glutathione bound to proteins, carbonyl protein and malondialdehyde concentrations were also noted, especially at the mitochondrial level. Enhanced carbonyl protein formation was also observed (p

Original languageEnglish
Pages (from-to)46-53
Number of pages8
JournalJournal of Hepatology
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 1998

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Keywords

  • Alcohol
  • Glutathione
  • Mitochondria
  • Nuclei
  • Sulfhydryl proteins
  • Tauroursodeoxycholic acid

ASJC Scopus subject areas

  • Gastroenterology

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