Ethanol-induced injury in rat primary cortical astrocytes involves oxidative stress: Effect of idebenone

Carolina Muscoli, Massimo Fresta, Venera Cardile, Maddalena Palumbo, Marcella Renis, Giovanni Puglisi, Donatella Paolino, Steven Nisticò, Domenicantonio Rotiroti, Vincenzo Mollace

Research output: Contribution to journalArticle

Abstract

Ethanol-induced neurological disorders have recently been characterised. Indeed, evidence has been collected indicating that chronic ethanol consumption leads to direct or indirect changes in the viability of central nervous system cells. Here we investigated the role of free radical overproduction in primary cortical rat astroglial cells undergoing chronic treatment with ethanol (100 μM). In particular, exposure of astroglial cell cultures to ethanol for 12 consecutive days produced an increased release of lactic dehydrogenase, a decrease on glutamine synthase activity being both effects accompanied by decrease in astroglial viability as detected by MTT (Thiazolyl Blue) test. These effects were accompanied by an increased formation of malondialdehyde (a marker of lipid peroxidation) and by abnormal formation of heat shock protein, being both effects antagonised by liposomally entrapped idebenone, a non-peptidyl free radical scavenger. Taken together, these results suggest that ethanol-induced injury on astroglial cells are mediated by abnormal formation of free radical species and this may represent a useful approach in the treatment of ethanol-related brain disorders.

Original languageEnglish
Pages (from-to)21-24
Number of pages4
JournalNeuroscience Letters
Volume329
Issue number1
DOIs
Publication statusPublished - Aug 23 2002

Keywords

  • Antioxidant
  • Astrocytes
  • Free radical species
  • Liposomally entrapped idebenone

ASJC Scopus subject areas

  • Neuroscience(all)

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    Muscoli, C., Fresta, M., Cardile, V., Palumbo, M., Renis, M., Puglisi, G., Paolino, D., Nisticò, S., Rotiroti, D., & Mollace, V. (2002). Ethanol-induced injury in rat primary cortical astrocytes involves oxidative stress: Effect of idebenone. Neuroscience Letters, 329(1), 21-24. https://doi.org/10.1016/S0304-3940(02)00567-0