Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats

W. C. De Bruijn, E. R. Boeve, P. R W A Van Run, P. P M C Van Miert, J. C. Romijn, C. F. Verkoelen, L. C. Cao, F. H. Schroder, D. J. Kok, F. Grases, P. Messa, S. R. Khan

Research output: Contribution to journalArticle

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Abstract

In a rat-model system, tubular crystal retention as a possible mechanism for the etiology of nephrolithiasis in man, was studied by conventional transmission electron microscopy. The animals were supplied for nine days with a crystal-inducing diet, with ethylene glycol plus NH4Cl in their drinking-water. After this induction period, a two day regime with fresh drinking-water was included, to allow crystals to be removed by spontaneous crystalluria. After aldehyde fixation of the rat kidneys, large crystals were seen inside the tubular lumen. The crystals were attached to cell surfaces and covered by neighboring epithelial cells. Some crystals were overgrown by several epithelial cells and underwent a process of so-called exotubulosis, resulting in free of cell-surrounded crystals in the interstitium, and possibly in crystals in Giant cells. To investigate the fate of the retained crystals, some animals were additionally exposed to a low-oxalate challenge from drinking water containing 0.1 volume per cent of ethylene glycol for 12 or 30 days, respectively. It was assumed that this would interfere with the retained intratubular or interstitial crystals, and allow the crystals to grow into mini-stones. This was not observed. After the oxalate challenge, no crystals were found to be retained in the tubules (free or covered by cells). Interstitial crystals were observed, but it remains to be demonstrated whether such crystals actually grow into mini-stones or that they are removed by the sterile inflammation process observed.

Original languageEnglish
Pages (from-to)541-550
Number of pages10
JournalScanning Microscopy
Volume8
Issue number3
Publication statusPublished - 1994

Fingerprint

etiology
oxalates
rats
Rats
calcium
Calcium
Crystals
crystals
drinking
Potable water
Ethylene glycol
cells
animals
glycols
interstitials
Animals
ethylene
rocks
water
diets

Keywords

  • Calcium oxalate
  • crystal-inducing diet
  • rat model
  • stone etiology
  • transmission electron microscopy

ASJC Scopus subject areas

  • Instrumentation

Cite this

De Bruijn, W. C., Boeve, E. R., Van Run, P. R. W. A., Van Miert, P. P. M. C., Romijn, J. C., Verkoelen, C. F., ... Khan, S. R. (1994). Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats. Scanning Microscopy, 8(3), 541-550.

Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats. / De Bruijn, W. C.; Boeve, E. R.; Van Run, P. R W A; Van Miert, P. P M C; Romijn, J. C.; Verkoelen, C. F.; Cao, L. C.; Schroder, F. H.; Kok, D. J.; Grases, F.; Messa, P.; Khan, S. R.

In: Scanning Microscopy, Vol. 8, No. 3, 1994, p. 541-550.

Research output: Contribution to journalArticle

De Bruijn, WC, Boeve, ER, Van Run, PRWA, Van Miert, PPMC, Romijn, JC, Verkoelen, CF, Cao, LC, Schroder, FH, Kok, DJ, Grases, F, Messa, P & Khan, SR 1994, 'Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats', Scanning Microscopy, vol. 8, no. 3, pp. 541-550.
De Bruijn WC, Boeve ER, Van Run PRWA, Van Miert PPMC, Romijn JC, Verkoelen CF et al. Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats. Scanning Microscopy. 1994;8(3):541-550.
De Bruijn, W. C. ; Boeve, E. R. ; Van Run, P. R W A ; Van Miert, P. P M C ; Romijn, J. C. ; Verkoelen, C. F. ; Cao, L. C. ; Schroder, F. H. ; Kok, D. J. ; Grases, F. ; Messa, P. ; Khan, S. R. / Etiology of experimental calcium oxalate monohydrate nephrolithiasis in rats. In: Scanning Microscopy. 1994 ; Vol. 8, No. 3. pp. 541-550.
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