Abstract
Imunocytochemical techniques were used to determine whether agonist-induced activation of μ-opioid receptors alters the number and distribution of μ-opioid receptor-positive cells in the rat cerebral cortex. In untreated rats, μ-opioid receptor immunoreactivity was localized to neuronal perikarya and dendrites and to neuropilar punctate structures. μ-Opioid receptor-positive neurons were mostly in layers II and III and exhibited a bipolar or bitufted morphology. In rats treated with the μ-opioid receptor agonist etorphine (0.1mg/kg intraperitoneally) and perfused after different survival periods, there was an enhancement of immunostaining for μ-opioid receptors observed at 15min, reaching a maximum at 60min, and which returned to normal at 480min. Etorphine-induced effects included an increase in the intensity of cellular and neuropil staining; statistical analysis showed that the number of μ-opioid receptor-positive cells in etorphine-treated groups was significantly higher than in controls or saline-treated rats. In animals that received both etorphine and the μ-opioid receptor antagonist naloxone, the pattern of μ-opioid receptors immunoreactivity was similar to that of untreated animals.This study shows that the number of μ-opioid receptor-positive cells is significantly increased following etorphine treatment and suggests that agonist treatment may be exploited to increased immunostaining of μ-opioid receptors and also of other G-protein coupled receptors. Copyright (C) 2000 IBRO.
| Original language | English |
|---|---|
| Pages (from-to) | 439-443 |
| Number of pages | 5 |
| Journal | Neuroscience |
| Volume | 100 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 27 2000 |
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Keywords
- Immunocytochemistry
- Neurons
- Opiate
- Rats
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Etorphine increases the number of μ-opioid receptor-positive cells in the cerebral cortex. / Melone, M.; Brecha, N. C.; Sternini, C.; Evans, C.; Conti, F.
In: Neuroscience, Vol. 100, No. 3, 27.09.2000, p. 439-443.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Etorphine increases the number of μ-opioid receptor-positive cells in the cerebral cortex
AU - Melone, M.
AU - Brecha, N. C.
AU - Sternini, C.
AU - Evans, C.
AU - Conti, F.
PY - 2000/9/27
Y1 - 2000/9/27
N2 - Imunocytochemical techniques were used to determine whether agonist-induced activation of μ-opioid receptors alters the number and distribution of μ-opioid receptor-positive cells in the rat cerebral cortex. In untreated rats, μ-opioid receptor immunoreactivity was localized to neuronal perikarya and dendrites and to neuropilar punctate structures. μ-Opioid receptor-positive neurons were mostly in layers II and III and exhibited a bipolar or bitufted morphology. In rats treated with the μ-opioid receptor agonist etorphine (0.1mg/kg intraperitoneally) and perfused after different survival periods, there was an enhancement of immunostaining for μ-opioid receptors observed at 15min, reaching a maximum at 60min, and which returned to normal at 480min. Etorphine-induced effects included an increase in the intensity of cellular and neuropil staining; statistical analysis showed that the number of μ-opioid receptor-positive cells in etorphine-treated groups was significantly higher than in controls or saline-treated rats. In animals that received both etorphine and the μ-opioid receptor antagonist naloxone, the pattern of μ-opioid receptors immunoreactivity was similar to that of untreated animals.This study shows that the number of μ-opioid receptor-positive cells is significantly increased following etorphine treatment and suggests that agonist treatment may be exploited to increased immunostaining of μ-opioid receptors and also of other G-protein coupled receptors. Copyright (C) 2000 IBRO.
AB - Imunocytochemical techniques were used to determine whether agonist-induced activation of μ-opioid receptors alters the number and distribution of μ-opioid receptor-positive cells in the rat cerebral cortex. In untreated rats, μ-opioid receptor immunoreactivity was localized to neuronal perikarya and dendrites and to neuropilar punctate structures. μ-Opioid receptor-positive neurons were mostly in layers II and III and exhibited a bipolar or bitufted morphology. In rats treated with the μ-opioid receptor agonist etorphine (0.1mg/kg intraperitoneally) and perfused after different survival periods, there was an enhancement of immunostaining for μ-opioid receptors observed at 15min, reaching a maximum at 60min, and which returned to normal at 480min. Etorphine-induced effects included an increase in the intensity of cellular and neuropil staining; statistical analysis showed that the number of μ-opioid receptor-positive cells in etorphine-treated groups was significantly higher than in controls or saline-treated rats. In animals that received both etorphine and the μ-opioid receptor antagonist naloxone, the pattern of μ-opioid receptors immunoreactivity was similar to that of untreated animals.This study shows that the number of μ-opioid receptor-positive cells is significantly increased following etorphine treatment and suggests that agonist treatment may be exploited to increased immunostaining of μ-opioid receptors and also of other G-protein coupled receptors. Copyright (C) 2000 IBRO.
KW - Immunocytochemistry
KW - Neurons
KW - Opiate
KW - Rats
UR - http://www.scopus.com/inward/record.url?scp=0034721510&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034721510&partnerID=8YFLogxK
U2 - 10.1016/S0306-4522(00)00307-9
DO - 10.1016/S0306-4522(00)00307-9
M3 - Article
C2 - 11098106
AN - SCOPUS:0034721510
VL - 100
SP - 439
EP - 443
JO - Neuroscience
JF - Neuroscience
SN - 0306-4522
IS - 3
ER -